##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI Aor51HI AscI
Asp718I AviII BbeI BbvCI BcgI
BclI BlpI Bpu1102I BseAI BsiWI
BsmBI BsmI BspEI BspHI BssSI
Bst1107I BstBI BstZ17I CelII CpoI
EagI EclXI Eco47III Eco52I EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MluI MroI MspA1I
NarI NdeI NotI NruI NspV
PacI PaeR7I PmeI PshAI Psp1406I
PvuII RcaI RsrII SacI SacII
SapI SbfI SfoI SfuI SnaBI
SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 700 GACGTC GACGT^C
AcyI 700 GACGTC G[GA]^CG[CT]C
AflII 3770 CTTAAG C^TTAAG
AflIII 3656 ACATGT A^C[GA][CT]GT
AhdI 2642 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 742 GTGCAC G^TGCAC
ApaLI 742 GTGCAC G^TGCAC
AspEI 2642 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1837 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1465 GGATCC G^GATCC
BbrPI 3242 CACGTG CAC^GTG
BfrI 3770 CTTAAG C^TTAAG
BsaBI 4068 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 700 GACGTC G[GA]^CG[CT]C
BsiEI 1155 CGATCG CG[GA][CT]^CG
BspDI 1153 ATCGAT AT^CGAT
BspLU11I 3656 ACATGT A^CATGT
BstAPI 1565 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 910 GGTCACC G^GT[ACGT]ACC
BstPI 910 GGTCACC G^GT[ACGT]ACC
ClaI 1153 ATCGAT AT^CGAT
DraIII 494 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 258 GACACCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1869 CCATGG C^C[GA][CT]GG
Eam1105I 2642 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 910 GGTCACC G^GT[ACGT]ACC
EcoRI 1930 GAATTC G^AATTC
EcoT22I 311 ATGCAT ATGCA^T
HaeII 92 AGCGCC [GA]GCGC^[CT]
HgaI 1359 GCGTC GACGC(5/10)
Hin1I 700 GACGTC G[GA]^CG[CT]C
MamI 4068 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1869 CCATGG C^CATGG
NsiI 311 ATGCAT ATGCA^T
NspI 3656 ACATGT [GA]CATG^[CT]
PflFI 1837 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3242 CACGTG CAC^GTG
PmlI 3242 CACGTG CAC^GTG
PvuI 1155 CGATCG CGAT^CG
SalI 1448 GTCGAC G^TCGAC
ScaI 2380 AGTACT AGT^ACT
SfiI 159 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 889 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1799 AGGCCT AGG^CCT
Tth111I 1837 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3997 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AgeI 1922 ACCGGT A^CCGGT
2791 ACCGGT
AseI 2227 ATTAAT AT^TAAT
4069 ATTAAT
AsnI 2227 ATTAAT AT^TAAT
4069 ATTAAT
BbsI 367 GAAGAC GAAGAC(2/6)
3307 GTCTTC
BciVI 1627 GTATCC GTATCC(6/5)
1823 GTATCC
BglI 160 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3768 GCCTTAAGGGC
BpuAI 367 GAAGAC GAAGAC(2/6)
3307 GTCTTC
BsaAI 1091 TACGTG [CT]AC^GT[GA]
3242 CACGTG
BsaWI 1922 ACCGGT [AT]^CCGG[AT]
2791 ACCGGT
Bsp1407I 524 TGTACA T^GTACA
4689 TGTACA
BsrGI 524 TGTACA T^GTACA
4689 TGTACA
EcoNI 573 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1632 CCTCCCTCAGG
HincII 1448 GTCGAC GT[CT]^[GA]AC
4233 GTCAAC
HindII 1448 GTCGAC GT[CT]^[GA]AC
4233 GTCAAC
HindIII 1061 AAGCTT A^AGCTT
2396 AAGCTT
MfeI 1383 CAATTG C^AATTG
3913 CAATTG
MunI 1383 CAATTG C^AATTG
3913 CAATTG
NaeI 890 GCCGGC GCC^GGC
1679 GCCGGC
NgoMIV 890 GCCGGC G^CCGGC
1679 GCCGGC
NheI 1970 GCTAGC G^CTAGC
4035 GCTAGC
PflMI 1059 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2917 CCAGGGCATGG
PinAI 1922 ACCGGT A^CCGGT
2791 ACCGGT
PshBI 2227 ATTAAT AT^TAAT
4069 ATTAAT
PstI 3375 CTGCAG CTGCA^G
4645 CTGCAG
SpeI 3037 ACTAGT A^CTAGT
4180 ACTAGT
SspBI 524 TGTACA T^GTACA
4689 TGTACA
SspI 4589 AATATT AAT^ATT
4990 AATATT
TaqI 1154 TCGA T^CGA
1449 TCGA
TthHB8I 1154 TCGA T^CGA
1449 TCGA
Van91I 1059 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2917 CCAGGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BanI BanII BbvI
BcnI BfaI BglII BlnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI XmnI