################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI Aor51HI AscI Asp718I AspI AviII AvrII BalI BbeI BbrPI BciVI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrFI BssHII BssSI BstBI BstEII BstPI Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HpaI KasI KpnI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 118 GGCGTC G[GA]^CG[CT]C AhdI 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3193 GTGCAC G^TGCAC AlwNI 4608 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 3193 GTGCAC G^TGCAC AseI 1882 ATTAAT AT^TAAT AsnI 1882 ATTAAT AT^TAAT Asp700I 946 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3299 GGATCC G^GATCC BanI 1126 GGTGCC G^G[CT][GA]CC BcgI 1626 CGATTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 308 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 118 GGCGTC G[GA]^CG[CT]C BsmBI 1708 GAGACG CGTCTC(1/5) BspHI 4478 TCATGA T^CATGA BspMI 3380 ACCTGC ACCTGC(4/8) BsrBI 12 CCGCTC CCGCTC(-3/-3) Bst1107I 1420 GTATAC GTA^TAC BstZ17I 1420 GTATAC GTA^TAC DsaI 318 CCGCGG C^C[GA][CT]GG EaeI 315 TGGCCG [CT]^GGCC[GA] Eam1105I 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 511 GATATC GAT^ATC HaeII 23 AGCGCC [GA]GCGC^[CT] Hin1I 118 GGCGTC G[GA]^CG[CT]C HincII 1870 GTTGAC GT[CT]^[GA]AC HindII 1870 GTTGAC GT[CT]^[GA]AC HindIII 4370 AAGCTT A^AGCTT KspI 318 CCGCGG CCGC^GG MfeI 5333 CAATTG C^AATTG MunI 5333 CAATTG C^AATTG PshBI 1882 ATTAAT AT^TAAT RcaI 4478 TCATGA T^CATGA SacII 318 CCGCGG CCGC^GG SapI 577 GAAGAGC GCTCTTC(1/4) SexAI 4019 ACCAGGT A^CC[AT]GGT SfiI 307 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 86 CCCGGG CCC^GGG SpeI 450 ACTAGT A^CTAGT XmaCI 86 CCCGGG C^CCGGG XmaI 86 CCCGGG C^CCGGG XmnI 946 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflII 851 CTTAAG C^TTAAG 1454 CTTAAG BfrI 851 CTTAAG C^TTAAG 1454 CTTAAG BsaBI 507 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5103 GATGGAAATC Bsp1407I 1069 TGTACA T^GTACA 1892 TGTACA BsrGI 1069 TGTACA T^GTACA 1892 TGTACA BstXI 1102 CCAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1782 CCAAAGCATTGG DraI 1474 TTTAAA TTT^AAA 4984 TTTAAA DraIII 134 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4018 CACCAGGTG EcoNI 806 CCTGGACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5315 CCTTTATGAGG MamI 507 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5103 GATGGAAATC MslI 1925 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4570 CATTGGCGTG PshAI 1268 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3689 GACTTGCGTC PvuII 284 CAGCTG CAG^CTG 3721 CAGCTG SacI 4053 GAGCTC GAGCT^C 5426 GAGCTC SphI 1667 GCATGC GCATG^C 2589 GCATGC SspBI 1069 TGTACA T^GTACA 1892 TGTACA SspI 4133 AATATT AAT^ATT 5580 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AocI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII