##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Aor51HI AscI Asp718I AspI
AviII AvrII BalI BbeI BbrPI
BciVI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrFI BssHII BssSI BstBI BstEII
BstPI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HpaI KasI
KpnI MluI MluNI MroI MscI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 118 GGCGTC G[GA]^CG[CT]C
AhdI 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3193 GTGCAC G^TGCAC
AlwNI 4608 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3193 GTGCAC G^TGCAC
AseI 1882 ATTAAT AT^TAAT
AsnI 1882 ATTAAT AT^TAAT
Asp700I 946 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3299 GGATCC G^GATCC
BanI 1126 GGTGCC G^G[CT][GA]CC
BcgI 1626 CGATTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 308 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 118 GGCGTC G[GA]^CG[CT]C
BsmBI 1708 GAGACG CGTCTC(1/5)
BspHI 4478 TCATGA T^CATGA
BspMI 3380 ACCTGC ACCTGC(4/8)
BsrBI 12 CCGCTC CCGCTC(-3/-3)
Bst1107I 1420 GTATAC GTA^TAC
BstZ17I 1420 GTATAC GTA^TAC
DsaI 318 CCGCGG C^C[GA][CT]GG
EaeI 315 TGGCCG [CT]^GGCC[GA]
Eam1105I 5387 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 511 GATATC GAT^ATC
HaeII 23 AGCGCC [GA]GCGC^[CT]
Hin1I 118 GGCGTC G[GA]^CG[CT]C
HincII 1870 GTTGAC GT[CT]^[GA]AC
HindII 1870 GTTGAC GT[CT]^[GA]AC
HindIII 4370 AAGCTT A^AGCTT
KspI 318 CCGCGG CCGC^GG
MfeI 5333 CAATTG C^AATTG
MunI 5333 CAATTG C^AATTG
PshBI 1882 ATTAAT AT^TAAT
RcaI 4478 TCATGA T^CATGA
SacII 318 CCGCGG CCGC^GG
SapI 577 GAAGAGC GCTCTTC(1/4)
SexAI 4019 ACCAGGT A^CC[AT]GGT
SfiI 307 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 86 CCCGGG CCC^GGG
SpeI 450 ACTAGT A^CTAGT
XmaCI 86 CCCGGG C^CCGGG
XmaI 86 CCCGGG C^CCGGG
XmnI 946 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 851 CTTAAG C^TTAAG
1454 CTTAAG
BfrI 851 CTTAAG C^TTAAG
1454 CTTAAG
BsaBI 507 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5103 GATGGAAATC
Bsp1407I 1069 TGTACA T^GTACA
1892 TGTACA
BsrGI 1069 TGTACA T^GTACA
1892 TGTACA
BstXI 1102 CCAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1782 CCAAAGCATTGG
DraI 1474 TTTAAA TTT^AAA
4984 TTTAAA
DraIII 134 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4018 CACCAGGTG
EcoNI 806 CCTGGACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5315 CCTTTATGAGG
MamI 507 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5103 GATGGAAATC
MslI 1925 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4570 CATTGGCGTG
PshAI 1268 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3689 GACTTGCGTC
PvuII 284 CAGCTG CAG^CTG
3721 CAGCTG
SacI 4053 GAGCTC GAGCT^C
5426 GAGCTC
SphI 1667 GCATGC GCATG^C
2589 GCATGC
SspBI 1069 TGTACA T^GTACA
1892 TGTACA
SspI 4133 AATATT AAT^ATT
5580 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII