##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AscI AseI AsnI AviII
AvrII BamHI BbrPI BclI BfrI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsmBI BspDI BspEI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MamI MluI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 360 GGTACC G^GTACC
AccII 90 CGCG CG^CG
AhdI 471 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2728 AGCGCT AGC^GCT
Asp700I 4425 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 360 GGTACC G^GTACC
AspEI 471 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 4596 CGAGAGACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4669 GTATCC GTATCC(6/5)
BlpI 2042 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2042 GCTCAGC GC^T[ACGT]AGC
BsiEI 3378 CGGTCG CG[GA][CT]^CG
BsiWI 19 CGTACG C^GTACG
Bsp1407I 2828 TGTACA T^GTACA
BspLU11I 4513 ACATGT A^CATGT
BsrGI 2828 TGTACA T^GTACA
BssSI 3016 CACGAG CACGAG(-5/-1)
BstUI 90 CGCG CG^CG
BstYI 2402 AGATCC [GA]^GATC[CT]
CelII 2042 GCTCAGC GC^T[ACGT]AGC
DraI 4983 TTTAAA TTT^AAA
DraIII 5114 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 471 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2728 AGCGCT AGC^GCT
EcoRI 5017 GAATTC G^AATTC
HincII 3205 GTCAAC GT[CT]^[GA]AC
HindII 3205 GTCAAC GT[CT]^[GA]AC
KpnI 360 GGTACC GGTAC^C
MfeI 2353 CAATTG C^AATTG
MflI 2402 AGATCC [GA]^GATC[CT]
MunI 2353 CAATTG C^AATTG
MvnI 90 CGCG CG^CG
NaeI 4339 GCCGGC GCC^GGC
NgoMIV 4339 GCCGGC G^CCGGC
SapI 2595 GCTCTTC GCTCTTC(1/4)
SspBI 2828 TGTACA T^GTACA
XbaI 5057 TCTAGA T^CTAGA
XhoII 2402 AGATCC [GA]^GATC[CT]
XmnI 4425 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 2435 AAATTT [GA]^AATT[CT]
5017 GAATTC
AflIII 271 ACGTGT A^C[GA][CT]GT
4513 ACATGT
Alw44I 722 GTGCAC G^TGCAC
2513 GTGCAC
AocI 1087 CCTCAGG CC^T[ACGT]AGG
1401 CCTGAGG
ApaLI 722 GTGCAC G^TGCAC
2513 GTGCAC
ApoI 2435 AAATTT [GA]^AATT[CT]
5017 GAATTC
AspI 3513 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTCTGTC
BbeI 42 GGCGCC GGCGC^C
3807 GGCGCC
BspHI 686 TCATGA T^CATGA
1025 TCATGA
BsrBI 3339 CCGCTC CCGCTC(-3/-3)
25 GAGCGG
BsrFI 4339 GCCGGC [GA]^CCGG[CT]
4737 ACCGGC
Bsu36I 1087 CCTCAGG CC^T[ACGT]AGG
1401 CCTGAGG
Cfr10I 4339 GCCGGC [GA]^CCGG[CT]
4737 ACCGGC
DrdI 327 GACATCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3843 GACCCTGCTGTC
Eco81I 1087 CCTCAGG CC^T[ACGT]AGG
1401 CCTGAGG
KasI 42 GGCGCC G^GCGCC
3807 GGCGCC
MslI 113 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2516 CACTTTTATG
NarI 42 GGCGCC GG^CGCC
3807 GGCGCC
NspI 616 GCATGT [GA]CATG^[CT]
4513 ACATGT
PflFI 3513 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTCTGTC
RcaI 686 TCATGA T^CATGA
1025 TCATGA
SfoI 42 GGCGCC GGC^GCC
3807 GGCGCC
SmaI 1943 CCCGGG CCC^GGG
3663 CCCGGG
Tth111I 3513 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3588 GACTCTGTC
XmaCI 1943 CCCGGG C^CCGGG
3663 CCCGGG
XmaI 1943 CCCGGG C^CCGGG
3663 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI