##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AvaI AviII AvrII BbeI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaBI BseAI BsiWI BsoBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstEII BstPI CfoI ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
HaeII HgaI HhaI HinP1I KasI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacI SapI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2454 GACGTC GACGT^C
AccII 1160 CGCG CG^CG
AcyI 2454 GACGTC G[GA]^CG[CT]C
Alw44I 4378 GTGCAC G^TGCAC
AocI 4292 CCTTAGG CC^T[ACGT]AGG
ApaLI 4378 GTGCAC G^TGCAC
AspI 356 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1636 TGGCCA TGG^CCA
BamHI 4123 GGATCC G^GATCC
BclI 606 TGATCA T^GATCA
BlpI 4303 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4303 GCTGAGC GC^T[ACGT]AGC
BsaHI 2454 GACGTC G[GA]^CG[CT]C
BsiEI 3252 CGGTCG CG[GA][CT]^CG
BsmBI 3402 CGTCTC CGTCTC(1/5)
BspHI 2071 TCATGA T^CATGA
BsrDI 1911 CATTGC GCAATG(2/0)
BsrFI 4403 ACCGGC [GA]^CCGG[CT]
Bst1107I 2309 GTATAC GTA^TAC
BstUI 1160 CGCG CG^CG
BstXI 1881 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2309 GTATAC GTA^TAC
Bsu36I 4292 CCTTAGG CC^T[ACGT]AGG
CelII 4303 GCTGAGC GC^T[ACGT]AGC
Cfr10I 4403 ACCGGC [GA]^CCGG[CT]
EaeI 1636 TGGCCA [CT]^GGCC[GA]
Eco81I 4292 CCTTAGG CC^T[ACGT]AGG
EcoNI 4750 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1592 GATATC GAT^ATC
FbaI 606 TGATCA T^GATCA
Hin1I 2454 GACGTC G[GA]^CG[CT]C
KspI 1159 CCGCGG CCGC^GG
MfeI 4136 CAATTG C^AATTG
MluNI 1636 TGGCCA TGG^CCA
MscI 1636 TGGCCA TGG^CCA
MunI 4136 CAATTG C^AATTG
MvnI 1160 CGCG CG^CG
NheI 253 GCTAGC G^CTAGC
PacI 2982 TTAATTAA TTAAT^TAA
PflFI 356 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4064 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2071 TCATGA T^CATGA
SacII 1159 CCGCGG CCGC^GG
SalI 3254 GTCGAC G^TCGAC
ScaI 2976 AGTACT AGT^ACT
SfiI 4362 GGCCCACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 3054 AATATT AAT^ATT
StuI 986 AGGCCT AGG^CCT
Tth111I 356 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AhdI 285 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3667 GACTGCTTGTC
ApaI 4314 GGGCCC GGGCC^C
5022 GGGCCC
AspEI 285 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3667 GACTGCTTGTC
BbrPI 66 CACGTG CAC^GTG
2166 CACGTG
BsaAI 66 CACGTG [CT]AC^GT[GA]
2166 CACGTG
BsaWI 3630 ACCGGA [AT]^CCGG[AT]
4268 ACCGGA
BspLU11I 2313 ACATGT A^CATGT
4463 ACATGT
BstAPI 1199 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1887 GCATGGCTTGC
DrdI 2454 GACGTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4437 GACCGGGATGTC
Eam1105I 285 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3667 GACTGCTTGTC
EcoRI 2875 GAATTC G^AATTC
5559 GAATTC
HpaI 4131 GTTAAC GTT^AAC
5181 GTTAAC
PmaCI 66 CACGTG CAC^GTG
2166 CACGTG
PmlI 66 CACGTG CAC^GTG
2166 CACGTG
PpuMI 3438 AGGACCC [GA]G^G[AT]CC[CT]
4512 GGGACCC
SmlI 1025 CTCAAG C^T[CT][GA]AG
2421 CTTGAG
SpeI 3095 ACTAGT A^CTAGT
5169 ACTAGT
SphI 3834 GCATGC GCATG^C
4847 GCATGC
XbaI 1384 TCTAGA T^CTAGA
5234 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I Asp718I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI