##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AviII
BbeI BbrPI BbsI BclI BfrI
BlpI Bpu1102I BpuAI BsiWI BssHII
Bst1107I BstZ17I CelII CpoI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 876 AAATTC [GA]^AATT[CT]
ApoI 876 AAATTC [GA]^AATT[CT]
Asp700I 3659 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 3219 GCTGAGG CCTCAGC(-5/-2)
BciVI 5019 GTATCC GTATCC(6/5)
BglI 542 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 409 CGACCG CG[GA][CT]^CG
BspDI 1522 ATCGAT AT^CGAT
BspLU11I 1418 ACATGT A^CATGT
BsrBI 3265 GAGCGG CCGCTC(-3/-3)
BsrDI 4904 CATTGC GCAATG(2/0)
BssSI 3101 CACGAG CACGAG(-5/-1)
BstAPI 1509 GCACGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2383 TTCGAA TT^CGAA
BstEII 4518 GGTCACC G^GT[ACGT]ACC
BstPI 4518 GGTCACC G^GT[ACGT]ACC
ClaI 1522 ATCGAT AT^CGAT
DraI 1933 TTTAAA TTT^AAA
DraIII 1510 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3984 GACCGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4518 GGTCACC G^GT[ACGT]ACC
KspI 69 CCGCGG CCGC^GG
MluI 4299 ACGCGT A^CGCGT
NspV 2383 TTCGAA TT^CGAA
PstI 153 CTGCAG CTGCA^G
SacII 69 CCGCGG CCGC^GG
SexAI 4399 ACCTGGT A^CC[AT]GGT
SfiI 541 GGCCAGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2383 TTCGAA TT^CGAA
StuI 962 AGGCCT AGG^CCT
XmnI 3659 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 61 GGCGTC G[GA]^CG[CT]C
3573 GACGCC
AflIII 1418 ACATGT A^C[GA][CT]GT
4299 ACGCGT
Alw44I 458 GTGCAC G^TGCAC
2607 GTGCAC
Aor51HI 1310 AGCGCT AGC^GCT
2489 AGCGCT
ApaLI 458 GTGCAC G^TGCAC
2607 GTGCAC
AspI 1200 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
BamHI 530 GGATCC G^GATCC
587 GGATCC
BcgI 1428 CGAGACAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2929 GCAGGAGAATCG
BglII 4270 AGATCT A^GATCT
4593 AGATCT
BsaBI 1803 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3633 GATGTCTATC
BsaHI 61 GGCGTC G[GA]^CG[CT]C
3573 GACGCC
Bsp1407I 1415 TGTACA T^GTACA
2209 TGTACA
BspHI 2052 TCATGA T^CATGA
4093 TCATGA
BsrGI 1415 TGTACA T^GTACA
2209 TGTACA
Eco47III 1310 AGCGCT AGC^GCT
2489 AGCGCT
Hin1I 61 GGCGTC G[GA]^CG[CT]C
3573 GACGCC
MamI 1803 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3633 GATGTCTATC
NspI 1149 ACATGC [GA]CATG^[CT]
1418 ACATGT
PaeR7I 837 CTCGAG C^TCGAG
1426 CTCGAG
PflFI 1200 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
RcaI 2052 TCATGA T^CATGA
4093 TCATGA
SmaI 1121 CCCGGG CCC^GGG
2462 CCCGGG
SspBI 1415 TGTACA T^GTACA
2209 TGTACA
Tth111I 1200 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3310 GACTCTGTC
XhoI 837 CTCGAG C^TCGAG
1426 CTCGAG
XmaCI 1121 CCCGGG C^CCGGG
2462 CCCGGG
XmaI 1121 CCCGGG C^CCGGG
2462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AccIII AciI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
AvrII BalI BanI BanII BbvI
BcnI BfaI BlnI BmyI BpmI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII