################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AflII AgeI AhdI Aor51HI AscI AspEI AvrII BbeI BfrI BlnI BlpI Bpu1102I BsiWI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PvuI SacII SapI SbfI SexAI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1251 GGTACC G^GTACC AccII 5925 CGCG CG^CG AccIII 737 TCCGGA T^CCGGA AclI 5043 AACGTT AA^CGTT Alw44I 3913 GTGCAC G^TGCAC ApaI 6574 GGGCCC GGGCC^C ApaLI 3913 GTGCAC G^TGCAC Asp700I 4448 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1251 GGTACC G^GTACC AviII 5389 TGCGCA TGC^GCA BamHI 609 GGATCC G^GATCC BcgI 4361 GCAACAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 4234 AGATCT A^GATCT BseAI 737 TCCGGA T^CCGGA BsmBI 6302 CGTCTC CGTCTC(1/5) Bsp1407I 3662 TGTACA T^GTACA BspDI 5373 ATCGAT AT^CGAT BspEI 737 TCCGGA T^CCGGA BspHI 5141 TCATGA T^CATGA BsrGI 3662 TGTACA T^GTACA BssSI 397 CTCGTG CACGAG(-5/-1) BstUI 5925 CGCG CG^CG ClaI 5373 ATCGAT AT^CGAT CpoI 5679 CGGACCG CG^G[AT]CCG EagI 4308 CGGCCG C^GGCCG EclXI 4308 CGGCCG C^GGCCG Eco52I 4308 CGGCCG C^GGCCG EcoNI 2895 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 5389 TGCGCA TGC^GCA HaeII 6106 AGCGCC [GA]GCGC^[CT] HindIII 3894 AAGCTT A^AGCTT HpaI 1692 GTTAAC GTT^AAC KpnI 1251 GGTACC GGTAC^C MroI 737 TCCGGA T^CCGGA MvnI 5925 CGCG CG^CG NaeI 5638 GCCGGC GCC^GGC NgoMIV 5638 GCCGGC G^CCGGC NheI 1089 GCTAGC G^CTAGC Psp1406I 5043 AACGTT AA^CGTT RcaI 5141 TCATGA T^CATGA RsrII 5679 CGGACCG CG^G[AT]CCG SacI 6135 GAGCTC GAGCT^C SalI 4330 GTCGAC G^TCGAC SfiI 1605 GGCCCTAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 4030 CCCGGG CCC^GGG SspBI 3662 TGTACA T^GTACA XmaCI 4030 CCCGGG C^CCGGG XmaI 4030 CCCGGG C^CCGGG XmnI 4448 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 4330 GTCGAC GT^[AC][GT]AC 5231 GTCTAC AcyI 2038 GACGCC G[GA]^CG[CT]C 5283 GACGCC AlwNI 721 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 5589 CAGTCTCTG AocI 4116 CCTCAGG CC^T[ACGT]AGG 4952 CCTCAGG AspI 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 5102 GACATGGTC BbsI 644 GTCTTC GAAGAC(2/6) 3803 GTCTTC BciVI 2201 GGATAC GTATCC(6/5) 392 GTATCC BclI 2093 TGATCA T^GATCA 5419 TGATCA BpuAI 644 GTCTTC GAAGAC(2/6) 3803 GTCTTC BsaHI 2038 GACGCC G[GA]^CG[CT]C 5283 GACGCC BspMI 2360 ACCTGC ACCTGC(4/8) 6558 ACCTGC BsrFI 4306 ACCGGC [GA]^CCGG[CT] 5638 GCCGGC BstAPI 4352 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5392 GCACTCTGTGC Bsu36I 4116 CCTCAGG CC^T[ACGT]AGG 4952 CCTCAGG Cfr10I 4306 ACCGGC [GA]^CCGG[CT] 5638 GCCGGC DraIII 5393 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 5940 CACTAAGTG DrdI 4884 GACCCTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5206 GACTGACTGGTC Eco81I 4116 CCTCAGG CC^T[ACGT]AGG 4952 CCTCAGG EcoRI 1082 GAATTC G^AATTC 3968 GAATTC FbaI 2093 TGATCA T^GATCA 5419 TGATCA Hin1I 2038 GACGCC G[GA]^CG[CT]C 5283 GACGCC PflFI 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 5102 GACATGGTC PshAI 637 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3255 GACAGTGGTC PstI 5960 CTGCAG CTGCA^G 6515 CTGCAG SpeI 2676 ACTAGT A^CTAGT 5007 ACTAGT SphI 4036 GCATGC GCATG^C 5401 GCATGC StuI 379 AGGCCT AGG^CCT 2251 AGGCCT Tth111I 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 5102 GACATGGTC XcmI 3940 CCATAGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5298 CCAGGTTCCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshBI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII