##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AflII AgeI AhdI Aor51HI
AscI AspEI AvrII BbeI BfrI
BlnI BlpI Bpu1102I BsiWI BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FseI KasI KspI
MfeI MluI MunI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PvuI SacII
SapI SbfI SexAI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1251 GGTACC G^GTACC
AccII 5925 CGCG CG^CG
AccIII 737 TCCGGA T^CCGGA
AclI 5043 AACGTT AA^CGTT
Alw44I 3913 GTGCAC G^TGCAC
ApaI 6574 GGGCCC GGGCC^C
ApaLI 3913 GTGCAC G^TGCAC
Asp700I 4448 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1251 GGTACC G^GTACC
AviII 5389 TGCGCA TGC^GCA
BamHI 609 GGATCC G^GATCC
BcgI 4361 GCAACAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4234 AGATCT A^GATCT
BseAI 737 TCCGGA T^CCGGA
BsmBI 6302 CGTCTC CGTCTC(1/5)
Bsp1407I 3662 TGTACA T^GTACA
BspDI 5373 ATCGAT AT^CGAT
BspEI 737 TCCGGA T^CCGGA
BspHI 5141 TCATGA T^CATGA
BsrGI 3662 TGTACA T^GTACA
BssSI 397 CTCGTG CACGAG(-5/-1)
BstUI 5925 CGCG CG^CG
ClaI 5373 ATCGAT AT^CGAT
CpoI 5679 CGGACCG CG^G[AT]CCG
EagI 4308 CGGCCG C^GGCCG
EclXI 4308 CGGCCG C^GGCCG
Eco52I 4308 CGGCCG C^GGCCG
EcoNI 2895 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 5389 TGCGCA TGC^GCA
HaeII 6106 AGCGCC [GA]GCGC^[CT]
HindIII 3894 AAGCTT A^AGCTT
HpaI 1692 GTTAAC GTT^AAC
KpnI 1251 GGTACC GGTAC^C
MroI 737 TCCGGA T^CCGGA
MvnI 5925 CGCG CG^CG
NaeI 5638 GCCGGC GCC^GGC
NgoMIV 5638 GCCGGC G^CCGGC
NheI 1089 GCTAGC G^CTAGC
Psp1406I 5043 AACGTT AA^CGTT
RcaI 5141 TCATGA T^CATGA
RsrII 5679 CGGACCG CG^G[AT]CCG
SacI 6135 GAGCTC GAGCT^C
SalI 4330 GTCGAC G^TCGAC
SfiI 1605 GGCCCTAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4030 CCCGGG CCC^GGG
SspBI 3662 TGTACA T^GTACA
XmaCI 4030 CCCGGG C^CCGGG
XmaI 4030 CCCGGG C^CCGGG
XmnI 4448 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 4330 GTCGAC GT^[AC][GT]AC
5231 GTCTAC
AcyI 2038 GACGCC G[GA]^CG[CT]C
5283 GACGCC
AlwNI 721 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
5589 CAGTCTCTG
AocI 4116 CCTCAGG CC^T[ACGT]AGG
4952 CCTCAGG
AspI 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACATGGTC
BbsI 644 GTCTTC GAAGAC(2/6)
3803 GTCTTC
BciVI 2201 GGATAC GTATCC(6/5)
392 GTATCC
BclI 2093 TGATCA T^GATCA
5419 TGATCA
BpuAI 644 GTCTTC GAAGAC(2/6)
3803 GTCTTC
BsaHI 2038 GACGCC G[GA]^CG[CT]C
5283 GACGCC
BspMI 2360 ACCTGC ACCTGC(4/8)
6558 ACCTGC
BsrFI 4306 ACCGGC [GA]^CCGG[CT]
5638 GCCGGC
BstAPI 4352 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5392 GCACTCTGTGC
Bsu36I 4116 CCTCAGG CC^T[ACGT]AGG
4952 CCTCAGG
Cfr10I 4306 ACCGGC [GA]^CCGG[CT]
5638 GCCGGC
DraIII 5393 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5940 CACTAAGTG
DrdI 4884 GACCCTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5206 GACTGACTGGTC
Eco81I 4116 CCTCAGG CC^T[ACGT]AGG
4952 CCTCAGG
EcoRI 1082 GAATTC G^AATTC
3968 GAATTC
FbaI 2093 TGATCA T^GATCA
5419 TGATCA
Hin1I 2038 GACGCC G[GA]^CG[CT]C
5283 GACGCC
PflFI 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACATGGTC
PshAI 637 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3255 GACAGTGGTC
PstI 5960 CTGCAG CTGCA^G
6515 CTGCAG
SpeI 2676 ACTAGT A^CTAGT
5007 ACTAGT
SphI 4036 GCATGC GCATG^C
5401 GCATGC
StuI 379 AGGCCT AGG^CCT
2251 AGGCCT
Tth111I 4461 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACATGGTC
XcmI 3940 CCATAGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5298 CCAGGTTCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaBI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PshBI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII