##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BclI BlnI BseAI BsiWI
Bsp1407I BspEI BsrGI BssHII BstBI
BstEII BstPI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI FseI MluI MroI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshBI PvuI RsrII SacI
SalI SexAI SfiI SfuI SnaBI
SphI SspBI SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3320 GACGTC GACGT^C
Acc65I 3797 GGTACC G^GTACC
AclI 2239 AACGTT AA^CGTT
Asp700I 3770 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3797 GGTACC G^GTACC
AspHI 3456 GAGCAC G[AT]GC[AT]^C
AviII 4021 TGCGCA TGC^GCA
BbrPI 4413 CACGTG CAC^GTG
BbvCI 4386 GCTGAGG CCTCAGC(-5/-2)
BcgI 4933 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4830 GTATCC GTATCC(6/5)
BsaAI 4413 CACGTG [CT]AC^GT[GA]
BsaBI 2640 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2246 CGACCG CG[GA][CT]^CG
BsiHKAI 3456 GAGCAC G[AT]GC[AT]^C
BsmI 4653 GCATTC GAATGC(1/-1)
BspLU11I 2860 ACATGT A^CATGT
BssSI 4288 CTCGTG CACGAG(-5/-1)
Bst1107I 4679 GTATAC GTA^TAC
BstZ17I 4679 GTATAC GTA^TAC
DraIII 1470 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3162 GACTGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 981 GATATC GAT^ATC
EcoT22I 3156 ATGCAT ATGCA^T
FspI 4021 TGCGCA TGC^GCA
HincII 3140 GTTAAC GT[CT]^[GA]AC
HindII 3140 GTTAAC GT[CT]^[GA]AC
HindIII 310 AAGCTT A^AGCTT
HpaI 3140 GTTAAC GTT^AAC
KpnI 3797 GGTACC GGTAC^C
KspI 43 CCGCGG CCGC^GG
MamI 2640 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3374 CAATTG C^AATTG
MunI 3374 CAATTG C^AATTG
NaeI 2495 GCCGGC GCC^GGC
NgoMIV 2495 GCCGGC G^CCGGC
NsiI 3156 ATGCAT ATGCA^T
PflMI 2460 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4413 CACGTG CAC^GTG
PmlI 4413 CACGTG CAC^GTG
Psp1406I 2239 AACGTT AA^CGTT
SacII 43 CCGCGG CCGC^GG
SbfI 2055 CCTGCAGG CCTGCA^GG
SgrAI 475 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3759 ACTAGT A^CTAGT
Sse8387I 2055 CCTGCAGG CCTGCA^GG
Van91I 2460 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3770 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AgeI 1111 ACCGGT A^CCGGT
1409 ACCGGT
BamHI 660 GGATCC G^GATCC
2366 GGATCC
BbeI 837 GGCGCC GGCGC^C
1706 GGCGCC
BbsI 2142 GAAGAC GAAGAC(2/6)
3317 GAAGAC
BglII 688 AGATCT A^GATCT
1514 AGATCT
BlpI 4610 GCTTAGC GC^T[ACGT]AGC
5118 GCTGAGC
Bpu1102I 4610 GCTTAGC GC^T[ACGT]AGC
5118 GCTGAGC
BpuAI 2142 GAAGAC GAAGAC(2/6)
3317 GAAGAC
BsmBI 1074 GAGACG CGTCTC(1/5)
1952 GAGACG
BspDI 2944 ATCGAT AT^CGAT
3642 ATCGAT
BspHI 1484 TCATGA T^CATGA
4855 TCATGA
BspMI 3947 ACCTGC ACCTGC(4/8)
5029 ACCTGC
BsrBI 3894 GAGCGG CCGCTC(-3/-3)
3984 GAGCGG
CelII 4610 GCTTAGC GC^T[ACGT]AGC
5118 GCTGAGC
ClaI 2944 ATCGAT AT^CGAT
3642 ATCGAT
EcoRI 952 GAATTC G^AATTC
5644 GAATTC
KasI 837 GGCGCC G^GCGCC
1706 GGCGCC
NarI 837 GGCGCC GG^CGCC
1706 GGCGCC
NcoI 4471 CCATGG C^CATGG
5239 CCATGG
NdeI 4296 CATATG CA^TATG
5562 CATATG
NheI 4032 GCTAGC G^CTAGC
5261 GCTAGC
NspI 199 GCATGT [GA]CATG^[CT]
2860 ACATGT
PinAI 1111 ACCGGT A^CCGGT
1409 ACCGGT
RcaI 1484 TCATGA T^CATGA
4855 TCATGA
SapI 3054 GAAGAGC GCTCTTC(1/4)
4232 GCTCTTC
ScaI 3762 AGTACT AGT^ACT
4351 AGTACT
SfoI 837 GGCGCC GGC^GCC
1706 GGCGCC
SmaI 461 CCCGGG CCC^GGG
2175 CCCGGG
XcmI 2836 CCAACTTCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3262 CCAAGCCCCGAATGG
XmaCI 461 CCCGGG C^CCGGG
2175 CCCGGG
XmaI 461 CCCGGG C^CCGGG
2175 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApaI ApoI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII