##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AccIII      AgeI        ApaI        AscI        
AviII       AvrII       BanII       BbrPI       BglI        
BglII       BlnI        BsaWI       BseAI       BsgI        
BsiWI       BspDI       BspEI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraII       EagI        EclXI       Eco52I      
EcoO109I    EcoO65I     FseI        FspI        HgaI        
HpaI        MluI        MroI        NotI        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       RsrII       SacI        SalI        
SapI        SbfI        ScaI        SfiI        SmaI        
SpeI        Sse8387I    StuI        Van91I      XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

Acc65I        592  GGTACC               G^GTACC
AcyI          174  GGCGCC               G[GA]^CG[CT]C
AhdI         4424  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        621  GTGCAC               G^TGCAC
Aor51HI      1595  AGCGCT               AGC^GCT
ApaLI         621  GTGCAC               G^TGCAC
Asp718I       592  GGTACC               G^GTACC
AspEI        4424  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2653  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         5204  CTCGAG               C^[CT]CG[GA]G
BalI         3196  TGGCCA               TGG^CCA
BbeI          174  GGCGCC               GGCGC^C
BbvCI         465  CCTCAGC              CCTCAGC(-5/-2)
BcgI         4796  CGATTTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          734  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      734  GCTAAGC              GC^T[ACGT]AGC
BsaAI         254  TACGTA               [CT]AC^GT[GA]
BsaBI        2690  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         174  GGCGCC               G[GA]^CG[CT]C
BseRI          52  CTCCTC               GAGGAG(10/8)
BsoBI        5204  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     2164  TGTACA               T^GTACA
BsrGI        2164  TGTACA               T^GTACA
BstBI        4218  TTCGAA               TT^CGAA
CelII         734  GCTAAGC              GC^T[ACGT]AGC
DraIII       2629  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          384  GACCCCAACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         3196  TGGCCA               [CT]^GGCC[GA]
Eam1105I     4424  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1595  AGCGCT               AGC^GCT
EcoNI        5177  CCTTAATTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        4333  GAATTC               G^AATTC
EcoRV        1230  GATATC               GAT^ATC
Hin1I         174  GGCGCC               G[GA]^CG[CT]C
HindIII       689  AAGCTT               A^AGCTT
KasI          174  GGCGCC               G^GCGCC
KpnI          592  GGTACC               GGTAC^C
KspI          473  CCGCGG               CCGC^GG
MamI         2690  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4514  CAATTG               C^AATTG
MluNI        3196  TGGCCA               TGG^CCA
MscI         3196  TGGCCA               TGG^CCA
MunI         4514  CAATTG               C^AATTG
NaeI          435  GCCGGC               GCC^GGC
NarI          174  GGCGCC               GG^CGCC
NcoI         1239  CCATGG               C^CATGG
NdeI         2600  CATATG               CA^TATG
NgoMIV        435  GCCGGC               G^CCGGC
NheI         2915  GCTAGC               G^CTAGC
NruI          351  TCGCGA               TCG^CGA
NspV         4218  TTCGAA               TT^CGAA
PaeR7I       5204  CTCGAG               C^TCGAG
PflFI        2653  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         4206  CTGCAG               CTGCA^G
PvuI         3779  CGATCG               CGAT^CG
SacII         473  CCGCGG               CCGC^GG
SexAI         569  ACCAGGT              A^CC[AT]GGT
SfoI          174  GGCGCC               GGC^GCC
SfuI         4218  TTCGAA               TT^CGAA
SgrAI         167  CGCCGGTG             C[GA]^CCGG[CT]G
SnaBI         254  TACGTA               TAC^GTA
SspBI        2164  TGTACA               T^GTACA
SwaI         3741  ATTTAAAT             ATTT^AAAT
Tth111I      2653  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5371  TCTAGA               T^CTAGA
XcmI         1059  CCAGGGAGAGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         5204  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AclI         2330  AACGTT               AA^CGTT
             3452  AACGTT              
AflII        3524  CTTAAG               C^TTAAG
             3685  CTTAAG              
AflIII       3870  ACATGT               A^C[GA][CT]GT
             5258  ACATGT              
Asp700I       510  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2432  GAAGCCCTTC          
BamHI         149  GGATCC               G^GATCC
             2408  GGATCC              
BcnI          107  CCCGG                CC^[GC]GG
             4224  CCGGG               
BfrI         3524  CTTAAG               C^TTAAG
             3685  CTTAAG              
BsaI         2524  GAGACC               GGTCTC(1/5)
             4906  GGTCTC              
BsiEI         264  CGACCG               CG[GA][CT]^CG
             3779  CGATCG              
BsmBI        4364  CGTCTC               CGTCTC(1/5)
             4711  GAGACG              
BspHI        2122  TCATGA               T^CATGA
             3430  TCATGA              
BspLU11I     3870  ACATGT               A^CATGT
             5258  ACATGT              
BsrFI         168  GCCGGT               [GA]^CCGG[CT]
              435  GCCGGC              
Cfr10I        168  GCCGGT               [GA]^CCGG[CT]
              435  GCCGGC              
DsaI          473  CCGCGG               C^C[GA][CT]GG
             1239  CCATGG              
HincII        121  GTTGAC               GT[CT]^[GA]AC
             4159  GTTGAC              
HindII        121  GTTGAC               GT[CT]^[GA]AC
             4159  GTTGAC              
NciI          107  CCCGG                CC^[GC]GG
             4224  CCGGG               
Psp1406I     2330  AACGTT               AA^CGTT
             3452  AACGTT              
PvuII        3200  CAGCTG               CAG^CTG
             3755  CAGCTG              
RcaI         2122  TCATGA               T^CATGA
             3430  TCATGA              
SphI         2637  GCATGC               GCATG^C
             3614  GCATGC              
XmnI          510  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2432  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
AseI        AsnI        AspHI       AvaII       BanI        
BbsI        BbvI        BciVI       BclI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrBI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EarI        
Eco81I      EcoRII      EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII