##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AgeI ApaI AscI
AviII AvrII BanII BbrPI BglI
BglII BlnI BsaWI BseAI BsgI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraII EagI EclXI Eco52I
EcoO109I EcoO65I FseI FspI HgaI
HpaI MluI MroI NotI PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI RsrII SacI SalI
SapI SbfI ScaI SfiI SmaI
SpeI Sse8387I StuI Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 592 GGTACC G^GTACC
AcyI 174 GGCGCC G[GA]^CG[CT]C
AhdI 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 621 GTGCAC G^TGCAC
Aor51HI 1595 AGCGCT AGC^GCT
ApaLI 621 GTGCAC G^TGCAC
Asp718I 592 GGTACC G^GTACC
AspEI 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5204 CTCGAG C^[CT]CG[GA]G
BalI 3196 TGGCCA TGG^CCA
BbeI 174 GGCGCC GGCGC^C
BbvCI 465 CCTCAGC CCTCAGC(-5/-2)
BcgI 4796 CGATTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 734 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 734 GCTAAGC GC^T[ACGT]AGC
BsaAI 254 TACGTA [CT]AC^GT[GA]
BsaBI 2690 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 174 GGCGCC G[GA]^CG[CT]C
BseRI 52 CTCCTC GAGGAG(10/8)
BsoBI 5204 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2164 TGTACA T^GTACA
BsrGI 2164 TGTACA T^GTACA
BstBI 4218 TTCGAA TT^CGAA
CelII 734 GCTAAGC GC^T[ACGT]AGC
DraIII 2629 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 384 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3196 TGGCCA [CT]^GGCC[GA]
Eam1105I 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1595 AGCGCT AGC^GCT
EcoNI 5177 CCTTAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4333 GAATTC G^AATTC
EcoRV 1230 GATATC GAT^ATC
Hin1I 174 GGCGCC G[GA]^CG[CT]C
HindIII 689 AAGCTT A^AGCTT
KasI 174 GGCGCC G^GCGCC
KpnI 592 GGTACC GGTAC^C
KspI 473 CCGCGG CCGC^GG
MamI 2690 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4514 CAATTG C^AATTG
MluNI 3196 TGGCCA TGG^CCA
MscI 3196 TGGCCA TGG^CCA
MunI 4514 CAATTG C^AATTG
NaeI 435 GCCGGC GCC^GGC
NarI 174 GGCGCC GG^CGCC
NcoI 1239 CCATGG C^CATGG
NdeI 2600 CATATG CA^TATG
NgoMIV 435 GCCGGC G^CCGGC
NheI 2915 GCTAGC G^CTAGC
NruI 351 TCGCGA TCG^CGA
NspV 4218 TTCGAA TT^CGAA
PaeR7I 5204 CTCGAG C^TCGAG
PflFI 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 4206 CTGCAG CTGCA^G
PvuI 3779 CGATCG CGAT^CG
SacII 473 CCGCGG CCGC^GG
SexAI 569 ACCAGGT A^CC[AT]GGT
SfoI 174 GGCGCC GGC^GCC
SfuI 4218 TTCGAA TT^CGAA
SgrAI 167 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 254 TACGTA TAC^GTA
SspBI 2164 TGTACA T^GTACA
SwaI 3741 ATTTAAAT ATTT^AAAT
Tth111I 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5371 TCTAGA T^CTAGA
XcmI 1059 CCAGGGAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5204 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AclI 2330 AACGTT AA^CGTT
3452 AACGTT
AflII 3524 CTTAAG C^TTAAG
3685 CTTAAG
AflIII 3870 ACATGT A^C[GA][CT]GT
5258 ACATGT
Asp700I 510 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2432 GAAGCCCTTC
BamHI 149 GGATCC G^GATCC
2408 GGATCC
BcnI 107 CCCGG CC^[GC]GG
4224 CCGGG
BfrI 3524 CTTAAG C^TTAAG
3685 CTTAAG
BsaI 2524 GAGACC GGTCTC(1/5)
4906 GGTCTC
BsiEI 264 CGACCG CG[GA][CT]^CG
3779 CGATCG
BsmBI 4364 CGTCTC CGTCTC(1/5)
4711 GAGACG
BspHI 2122 TCATGA T^CATGA
3430 TCATGA
BspLU11I 3870 ACATGT A^CATGT
5258 ACATGT
BsrFI 168 GCCGGT [GA]^CCGG[CT]
435 GCCGGC
Cfr10I 168 GCCGGT [GA]^CCGG[CT]
435 GCCGGC
DsaI 473 CCGCGG C^C[GA][CT]GG
1239 CCATGG
HincII 121 GTTGAC GT[CT]^[GA]AC
4159 GTTGAC
HindII 121 GTTGAC GT[CT]^[GA]AC
4159 GTTGAC
NciI 107 CCCGG CC^[GC]GG
4224 CCGGG
Psp1406I 2330 AACGTT AA^CGTT
3452 AACGTT
PvuII 3200 CAGCTG CAG^CTG
3755 CAGCTG
RcaI 2122 TCATGA T^CATGA
3430 TCATGA
SphI 2637 GCATGC GCATG^C
3614 GCATGC
XmnI 510 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2432 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaII BanI
BbsI BbvI BciVI BclI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII