################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AccIII AgeI ApaI AscI AviII AvrII BanII BbrPI BglI BglII BlnI BsaWI BseAI BsgI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraII EagI EclXI Eco52I EcoO109I EcoO65I FseI FspI HgaI HpaI MluI MroI NotI PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI RsrII SacI SalI SapI SbfI ScaI SfiI SmaI SpeI Sse8387I StuI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 592 GGTACC G^GTACC AcyI 174 GGCGCC G[GA]^CG[CT]C AhdI 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 621 GTGCAC G^TGCAC Aor51HI 1595 AGCGCT AGC^GCT ApaLI 621 GTGCAC G^TGCAC Asp718I 592 GGTACC G^GTACC AspEI 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5204 CTCGAG C^[CT]CG[GA]G BalI 3196 TGGCCA TGG^CCA BbeI 174 GGCGCC GGCGC^C BbvCI 465 CCTCAGC CCTCAGC(-5/-2) BcgI 4796 CGATTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 734 GCTAAGC GC^T[ACGT]AGC Bpu1102I 734 GCTAAGC GC^T[ACGT]AGC BsaAI 254 TACGTA [CT]AC^GT[GA] BsaBI 2690 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 174 GGCGCC G[GA]^CG[CT]C BseRI 52 CTCCTC GAGGAG(10/8) BsoBI 5204 CTCGAG C^[CT]CG[GA]G Bsp1407I 2164 TGTACA T^GTACA BsrGI 2164 TGTACA T^GTACA BstBI 4218 TTCGAA TT^CGAA CelII 734 GCTAAGC GC^T[ACGT]AGC DraIII 2629 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 384 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 3196 TGGCCA [CT]^GGCC[GA] Eam1105I 4424 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1595 AGCGCT AGC^GCT EcoNI 5177 CCTTAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 4333 GAATTC G^AATTC EcoRV 1230 GATATC GAT^ATC Hin1I 174 GGCGCC G[GA]^CG[CT]C HindIII 689 AAGCTT A^AGCTT KasI 174 GGCGCC G^GCGCC KpnI 592 GGTACC GGTAC^C KspI 473 CCGCGG CCGC^GG MamI 2690 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4514 CAATTG C^AATTG MluNI 3196 TGGCCA TGG^CCA MscI 3196 TGGCCA TGG^CCA MunI 4514 CAATTG C^AATTG NaeI 435 GCCGGC GCC^GGC NarI 174 GGCGCC GG^CGCC NcoI 1239 CCATGG C^CATGG NdeI 2600 CATATG CA^TATG NgoMIV 435 GCCGGC G^CCGGC NheI 2915 GCTAGC G^CTAGC NruI 351 TCGCGA TCG^CGA NspV 4218 TTCGAA TT^CGAA PaeR7I 5204 CTCGAG C^TCGAG PflFI 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 4206 CTGCAG CTGCA^G PvuI 3779 CGATCG CGAT^CG SacII 473 CCGCGG CCGC^GG SexAI 569 ACCAGGT A^CC[AT]GGT SfoI 174 GGCGCC GGC^GCC SfuI 4218 TTCGAA TT^CGAA SgrAI 167 CGCCGGTG C[GA]^CCGG[CT]G SnaBI 254 TACGTA TAC^GTA SspBI 2164 TGTACA T^GTACA SwaI 3741 ATTTAAAT ATTT^AAAT Tth111I 2653 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5371 TCTAGA T^CTAGA XcmI 1059 CCAGGGAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 5204 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AclI 2330 AACGTT AA^CGTT 3452 AACGTT AflII 3524 CTTAAG C^TTAAG 3685 CTTAAG AflIII 3870 ACATGT A^C[GA][CT]GT 5258 ACATGT Asp700I 510 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2432 GAAGCCCTTC BamHI 149 GGATCC G^GATCC 2408 GGATCC BcnI 107 CCCGG CC^[GC]GG 4224 CCGGG BfrI 3524 CTTAAG C^TTAAG 3685 CTTAAG BsaI 2524 GAGACC GGTCTC(1/5) 4906 GGTCTC BsiEI 264 CGACCG CG[GA][CT]^CG 3779 CGATCG BsmBI 4364 CGTCTC CGTCTC(1/5) 4711 GAGACG BspHI 2122 TCATGA T^CATGA 3430 TCATGA BspLU11I 3870 ACATGT A^CATGT 5258 ACATGT BsrFI 168 GCCGGT [GA]^CCGG[CT] 435 GCCGGC Cfr10I 168 GCCGGT [GA]^CCGG[CT] 435 GCCGGC DsaI 473 CCGCGG C^C[GA][CT]GG 1239 CCATGG HincII 121 GTTGAC GT[CT]^[GA]AC 4159 GTTGAC HindII 121 GTTGAC GT[CT]^[GA]AC 4159 GTTGAC NciI 107 CCCGG CC^[GC]GG 4224 CCGGG Psp1406I 2330 AACGTT AA^CGTT 3452 AACGTT PvuII 3200 CAGCTG CAG^CTG 3755 CAGCTG RcaI 2122 TCATGA T^CATGA 3430 TCATGA SphI 2637 GCATGC GCATG^C 3614 GCATGC XmnI 510 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2432 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaII BanI BbsI BbvI BciVI BclI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI EarI Eco81I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII