##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BbrPI BcgI BclI BcnI
BfrI BglI BlnI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BssHII
BstBI ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I FbaI FseI FspI KpnI
Ksp632I KspI MluI MroI NaeI
NciI NgoMIV NheI NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SmaI SphI Sse8387I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1256 GACGTC GACGT^C
AccII 108 CGCG CG^CG
AflIII 4120 ACATGT A^C[GA][CT]GT
AlwNI 1838 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 2459 GTGCTC G[AT]GC[AT]^C
BbeI 4538 GGCGCC GGCGC^C
BciVI 2613 GTATCC GTATCC(6/5)
BlpI 4769 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4769 GCTTAGC GC^T[ACGT]AGC
BsaAI 4134 TACGTA [CT]AC^GT[GA]
BsaBI 1085 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3062 TCCGGT [AT]^CCGG[AT]
BsgI 5305 CTGCAC GTGCAG(16/14)
BsiHKAI 2459 GTGCTC G[AT]GC[AT]^C
BsmBI 1254 GAGACG CGTCTC(1/5)
Bsp1407I 3356 TGTACA T^GTACA
BspHI 2174 TCATGA T^CATGA
BspLU11I 4120 ACATGT A^CATGT
BspMI 3323 GCAGGT ACCTGC(4/8)
BsrBI 2501 GAGCGG CCGCTC(-3/-3)
BsrFI 103 ACCGGC [GA]^CCGG[CT]
BsrGI 3356 TGTACA T^GTACA
BssSI 739 CACGAG CACGAG(-5/-1)
Bst1107I 5172 GTATAC GTA^TAC
BstEII 99 GGTCACC G^GT[ACGT]ACC
BstPI 99 GGTCACC G^GT[ACGT]ACC
BstUI 108 CGCG CG^CG
BstZ17I 5172 GTATAC GTA^TAC
CelII 4769 GCTTAGC GC^T[ACGT]AGC
Cfr10I 103 ACCGGC [GA]^CCGG[CT]
DraII 3784 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 3784 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 99 GGTCACC G^GT[ACGT]ACC
HaeII 4538 GGCGCC [GA]GCGC^[CT]
KasI 4538 GGCGCC G^GCGCC
MamI 1085 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 596 CAATTG C^AATTG
MunI 596 CAATTG C^AATTG
MvnI 108 CGCG CG^CG
NarI 4538 GGCGCC GG^CGCC
NcoI 4054 CCATGG C^CATGG
PacI 4178 TTAATTAA TTAAT^TAA
PflMI 3572 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2174 TCATGA T^CATGA
SfoI 4538 GGCGCC GGC^GCC
SgrAI 102 CACCGGCG C[GA]^CCGG[CT]G
SmlI 4480 CTCAAG C^T[CT][GA]AG
SnaBI 4134 TACGTA TAC^GTA
SpeI 2690 ACTAGT A^CTAGT
SspBI 3356 TGTACA T^GTACA
StuI 50 AGGCCT AGG^CCT
SwaI 1394 ATTTAAAT ATTT^AAAT
Van91I 3572 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2617 CCAAGAATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2704 GTCTAC GT^[AC][GT]AC
5172 GTATAC
AocI 2549 CCTGAGG CC^T[ACGT]AGG
3671 CCTGAGG
AspI 3179 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3536 GACATTGTC
BamHI 2821 GGATCC G^GATCC
3059 GGATCC
BanI 714 GGCACC G^G[CT][GA]CC
4538 GGCGCC
BanII 63 GGGCTC G[GA]GC[CT]^C
4477 GGGCTC
BbvCI 4361 CCTCAGC CCTCAGC(-5/-2)
72 GCTGAGG
BglII 681 AGATCT A^GATCT
2577 AGATCT
BsaI 4714 GAGACC GGTCTC(1/5)
4463 GGTCTC
Bsu36I 2549 CCTGAGG CC^T[ACGT]AGG
3671 CCTGAGG
CfoI 107 GCGC GCG^C
4539 GCGC
Eco81I 2549 CCTGAGG CC^T[ACGT]AGG
3671 CCTGAGG
EcoRI 362 GAATTC G^AATTC
3501 GAATTC
EcoRV 4697 GATATC GAT^ATC
5347 GATATC
HapII 104 CCGG C^CGG
3063 CCGG
HhaI 107 GCGC GCG^C
4539 GCGC
HinP1I 107 GCGC G^CGC
4539 GCGC
HpaI 2048 GTTAAC GTT^AAC
5037 GTTAAC
HpaII 104 CCGG C^CGG
3063 CCGG
MspI 104 CCGG C^CGG
3063 CCGG
PflFI 3179 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3536 GACATTGTC
PvuII 1309 CAGCTG CAG^CTG
3982 CAGCTG
ScaI 2247 AGTACT AGT^ACT
2979 AGTACT
Tth111I 3179 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3536 GACATTGTC
XbaI 1194 TCTAGA T^CTAGA
1782 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BalI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EcoNI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HgaI Hin1I HincII HindII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI