##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AspI
AviII BbeI BbrPI BbvCI BcgI
BfrI BglI BlpI Bpu1102I BsiWI
BsmBI BspDI BssSI BstBI Bsu36I
CelII ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoRV FseI FspI
KasI KspI MluI NarI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 902 TCCGGA T^CCGGA
Alw44I 4254 GTGCAC G^TGCAC
AlwNI 97 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 766 GGGCCC GGGCC^C
ApaLI 4254 GTGCAC G^TGCAC
Asp700I 1879 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2919 CCTAGG C^CTAGG
BciVI 3724 GGATAC GTATCC(6/5)
BlnI 2919 CCTAGG C^CTAGG
BseAI 902 TCCGGA T^CCGGA
BsiEI 136 CGGCCG CG[GA][CT]^CG
BspEI 902 TCCGGA T^CCGGA
BspHI 2030 TCATGA T^CATGA
BssHII 16 GCGCGC G^CGCGC
BstXI 3277 CCAATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 665 GACTCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 136 CGGCCG C^GGCCG
EclXI 136 CGGCCG C^GGCCG
Eco52I 136 CGGCCG C^GGCCG
EcoNI 4143 CCTACATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4742 GAATTC G^AATTC
HaeII 1582 AGCGCC [GA]GCGC^[CT]
MroI 902 TCCGGA T^CCGGA
NcoI 3455 CCATGG C^CATGG
NdeI 4930 CATATG CA^TATG
PflMI 988 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 581 CAGCTG CAG^CTG
RcaI 2030 TCATGA T^CATGA
SgrAI 784 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 3563 TACGTA TAC^GTA
SpeI 1864 ACTAGT A^CTAGT
Van91I 988 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4523 TCTAGA T^CTAGA
XmnI 1879 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1991 GGTACC G^GTACC
4775 GGTACC
AcyI 782 GACGCC G[GA]^CG[CT]C
2527 GGCGTC
AseI 4539 ATTAAT AT^TAAT
4795 ATTAAT
AsnI 4539 ATTAAT AT^TAAT
4795 ATTAAT
Asp718I 1991 GGTACC G^GTACC
4775 GGTACC
AspHI 2375 GAGCAC G[AT]GC[AT]^C
4254 GTGCAC
BalI 914 TGGCCA TGG^CCA
3941 TGGCCA
BamHI 595 GGATCC G^GATCC
2727 GGATCC
BclI 1230 TGATCA T^GATCA
2949 TGATCA
BglII 502 AGATCT A^GATCT
3153 AGATCT
BsaAI 2378 CACGTA [CT]AC^GT[GA]
3563 TACGTA
BsaBI 2728 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4186 GATGAATATC
BsaHI 782 GACGCC G[GA]^CG[CT]C
2527 GGCGTC
BsaI 2287 GAGACC GGTCTC(1/5)
3384 GAGACC
BsaWI 733 ACCGGA [AT]^CCGG[AT]
902 TCCGGA
BsiHKAI 2375 GAGCAC G[AT]GC[AT]^C
4254 GTGCAC
BsmI 1839 GAATGC GAATGC(1/-1)
4813 GCATTC
BsrBI 685 CCGCTC CCGCTC(-3/-3)
716 CCGCTC
BstAPI 1937 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4693 GCAGTTCTTGC
DsaI 599 CCGTGG C^C[GA][CT]GG
3455 CCATGG
FbaI 1230 TGATCA T^GATCA
2949 TGATCA
Hin1I 782 GACGCC G[GA]^CG[CT]C
2527 GGCGTC
HpaI 3852 GTTAAC GTT^AAC
3912 GTTAAC
KpnI 1991 GGTACC GGTAC^C
4775 GGTACC
MamI 2728 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4186 GATGAATATC
MluNI 914 TGGCCA TGG^CCA
3941 TGGCCA
MscI 914 TGGCCA TGG^CCA
3941 TGGCCA
PshBI 4539 ATTAAT AT^TAAT
4795 ATTAAT
SmaI 770 CCCGGG CCC^GGG
3221 CCCGGG
XmaCI 770 CCCGGG C^CCGGG
3221 CCCGGG
XmaI 770 CCCGGG C^CCGGG
3221 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI Bst1107I BstEII BstF5I
BstNI BstPI BstUI BstYI BstZ17I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII