##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AscI AseI AsnI Asp718I
AviII BbrPI BcgI BciVI BclI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraIII EcoO65I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MluI MroI MvnI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 3859 CTTAAG C^TTAAG
AhdI 4991 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3034 GTGCAC G^TGCAC
Aor51HI 3502 AGCGCT AGC^GCT
ApaLI 3034 GTGCAC G^TGCAC
AspEI 4991 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3408 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 821 GAAGAC GAAGAC(2/6)
BfrI 3859 CTTAAG C^TTAAG
BglII 2478 AGATCT A^GATCT
BpuAI 821 GAAGAC GAAGAC(2/6)
BsiEI 1999 CGGCCG CG[GA][CT]^CG
BsmBI 51 CGTCTC CGTCTC(1/5)
BsrBI 2891 CCGCTC CCGCTC(-3/-3)
BstAPI 3330 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4506 TTTAAA TTT^AAA
DrdI 2633 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1999 CGGCCG C^GGCCG
Eam1105I 4991 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1999 CGGCCG C^GGCCG
Eco47III 3502 AGCGCT AGC^GCT
Eco52I 1999 CGGCCG C^GGCCG
EcoRV 4130 GATATC GAT^ATC
EcoT22I 109 ATGCAT ATGCA^T
MfeI 3025 CAATTG C^AATTG
MunI 3025 CAATTG C^AATTG
NcoI 970 CCATGG C^CATGG
NheI 749 GCTAGC G^CTAGC
NsiI 109 ATGCAT ATGCA^T
PflFI 3408 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2160 GAGCTC GAGCT^C
SapI 10 GCTCTTC GCTCTTC(1/4)
SbfI 3327 CCTGCAGG CCTGCA^GG
Sse8387I 3327 CCTGCAGG CCTGCA^GG
Tth111I 3408 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4085 GTCTAC GT^[AC][GT]AC
5366 GTAGAC
AcyI 3510 GGCGCC G[GA]^CG[CT]C
3895 GGCGCC
AflIII 1600 ACATGT A^C[GA][CT]GT
4445 ACATGT
Asp700I 1665 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4413 GAATTCCTTC
AvrII 4365 CCTAGG C^CTAGG
4439 CCTAGG
BamHI 3300 GGATCC G^GATCC
5399 GGATCC
BbeI 3510 GGCGCC GGCGC^C
3895 GGCGCC
BbvCI 723 CCTCAGC CCTCAGC(-5/-2)
5282 GCTGAGG
BlnI 4365 CCTAGG C^CTAGG
4439 CCTAGG
BlpI 2843 GCTCAGC GC^T[ACGT]AGC
5509 GCTCAGC
Bpu1102I 2843 GCTCAGC GC^T[ACGT]AGC
5509 GCTCAGC
BsaHI 3510 GGCGCC G[GA]^CG[CT]C
3895 GGCGCC
BspHI 1948 TCATGA T^CATGA
5272 TCATGA
BspLU11I 1600 ACATGT A^CATGT
4445 ACATGT
BsrDI 161 CATTGC GCAATG(2/0)
2411 CATTGC
CelII 2843 GCTCAGC GC^T[ACGT]AGC
5509 GCTCAGC
EcoRI 390 GAATTC G^AATTC
4413 GAATTC
HgaI 3843 GACGC GACGC(5/10)
5257 GCGTC
Hin1I 3510 GGCGCC G[GA]^CG[CT]C
3895 GGCGCC
HincII 2053 GTCAAC GT[CT]^[GA]AC
5288 GTTGAC
HindII 2053 GTCAAC GT[CT]^[GA]AC
5288 GTTGAC
KasI 3510 GGCGCC G^GCGCC
3895 GGCGCC
NarI 3510 GGCGCC GG^CGCC
3895 GGCGCC
PshAI 93 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2655 GACTTGAGTC
RcaI 1948 TCATGA T^CATGA
5272 TCATGA
SfoI 3510 GGCGCC GGC^GCC
3895 GGCGCC
SmlI 2657 CTTGAG C^T[CT][GA]AG
3859 CTTAAG
SspI 575 AATATT AAT^ATT
3123 AATATT
XcmI 3496 CCAGTGAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4601 CCAGCTAGGCCTTGG
XmnI 1665 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4413 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI