##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I ApaLI AscI AspEI
AviII BbeI BbrPI BciVI BclI
BfrI BsgI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I FbaI FseI FspI HindIII
HpaI KasI KspI MluI NarI
NheI NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Van91I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3665 GGTACC G^GTACC
AccII 2829 CGCG CG^CG
AccIII 4175 TCCGGA T^CCGGA
AcyI 80 GGCGTC G[GA]^CG[CT]C
AflIII 847 ACGTGT A^C[GA][CT]GT
AocI 2544 CCTGAGG CC^T[ACGT]AGG
Asp718I 3665 GGTACC G^GTACC
AspI 2065 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1559 CCTAGG C^CTAGG
BbvCI 3506 CCTCAGC CCTCAGC(-5/-2)
BcgI 1826 GCACGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3932 AGATCT A^GATCT
BlnI 1559 CCTAGG C^CTAGG
BlpI 2592 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2592 GCTCAGC GC^T[ACGT]AGC
BsaAI 846 TACGTG [CT]AC^GT[GA]
BsaHI 80 GGCGTC G[GA]^CG[CT]C
BseAI 4175 TCCGGA T^CCGGA
BsmBI 914 CGTCTC CGTCTC(1/5)
BspEI 4175 TCCGGA T^CCGGA
BspHI 4459 TCATGA T^CATGA
BsrDI 3363 GCAATG GCAATG(2/0)
BsrFI 2749 GCCGGC [GA]^CCGG[CT]
BstEII 5376 GGTGACC G^GT[ACGT]ACC
BstPI 5376 GGTGACC G^GT[ACGT]ACC
BstUI 2829 CGCG CG^CG
Bsu36I 2544 CCTGAGG CC^T[ACGT]AGG
CelII 2592 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2749 GCCGGC [GA]^CCGG[CT]
DraIII 1462 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1809 GACATGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2544 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5376 GGTGACC G^GT[ACGT]ACC
EcoT22I 537 ATGCAT ATGCA^T
Hin1I 80 GGCGTC G[GA]^CG[CT]C
HincII 2484 GTCAAC GT[CT]^[GA]AC
HindII 2484 GTCAAC GT[CT]^[GA]AC
KpnI 3665 GGTACC GGTAC^C
MfeI 2722 CAATTG C^AATTG
MroI 4175 TCCGGA T^CCGGA
MunI 2722 CAATTG C^AATTG
MvnI 2829 CGCG CG^CG
NaeI 2749 GCCGGC GCC^GGC
NgoMIV 2749 GCCGGC G^CCGGC
NsiI 537 ATGCAT ATGCA^T
PflFI 2065 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4459 TCATGA T^CATGA
ScaI 4637 AGTACT AGT^ACT
SexAI 2066 ACCAGGT A^CC[AT]GGT
SspI 4027 AATATT AAT^ATT
Tth111I 2065 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2061 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 409 AGCGCT AGC^GCT
1393 AGCGCT
AseI 5142 ATTAAT AT^TAAT
5623 ATTAAT
AsnI 5142 ATTAAT AT^TAAT
5623 ATTAAT
BamHI 2493 GGATCC G^GATCC
3148 GGATCC
BsaBI 526 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2469 GATGTCAATC
BsaWI 3963 ACCGGA [AT]^CCGG[AT]
4175 TCCGGA
DraI 4778 TTTAAA TTT^AAA
5680 TTTAAA
Eco47III 409 AGCGCT AGC^GCT
1393 AGCGCT
EcoRV 983 GATATC GAT^ATC
1416 GATATC
MamI 526 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2469 GATGTCAATC
NdeI 1704 CATATG CA^TATG
2760 CATATG
PaeR7I 4036 CTCGAG C^TCGAG
4703 CTCGAG
PshBI 5142 ATTAAT AT^TAAT
5623 ATTAAT
SacI 563 GAGCTC GAGCT^C
1594 GAGCTC
SapI 1282 GCTCTTC GCTCTTC(1/4)
5254 GCTCTTC
SfiI 1535 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3801 GGCCCCCTTGGCC
SmaI 3870 CCCGGG CCC^GGG
4483 CCCGGG
XcmI 2315 CCACATGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2633 CCAGCAGGAGTATGG
XhoI 4036 CTCGAG C^TCGAG
4703 CTCGAG
XmaCI 3870 CCCGGG C^CCGGG
4483 CCCGGG
XmaI 3870 CCCGGG C^CCGGG
4483 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI