##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BciVI BfrI BlnI BseAI BsiWI
BspDI BspEI BsrDI BssSI Bst1107I
BstBI BstZ17I ClaI Eco47III EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SacI SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SphI
Sse8387I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AccI 5051 GTAGAC GT^[AC][GT]AC
Alw44I 3465 GTGCAC G^TGCAC
ApaLI 3465 GTGCAC G^TGCAC
BamHI 2027 GGATCC G^GATCC
BclI 3046 TGATCA T^GATCA
BglII 2740 AGATCT A^GATCT
BsaAI 569 TACGTG [CT]AC^GT[GA]
BsaBI 184 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 875 ACCGGA [AT]^CCGG[AT]
BspLU11I 2527 ACATGT A^CATGT
BstAPI 3537 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 872 CGGACCG CG^G[AT]CCG
DraIII 515 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4147 GAATTC G^AATTC
FbaI 3046 TGATCA T^GATCA
MamI 184 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshAI 5038 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 872 CGGACCG CG^G[AT]CCG
SfiI 1486 GGCCCGTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4236 ACTAGT A^CTAGT
SwaI 4688 ATTTAAAT ATTT^AAAT
XbaI 3086 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 2567 AAATTC [GA]^AATT[CT]
4147 GAATTC
AhdI 773 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1606 GACAGTGCGTC
ApoI 2567 AAATTC [GA]^AATT[CT]
4147 GAATTC
Asp700I 2686 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4095 GAAAGACTTC
AspEI 773 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1606 GACAGTGCGTC
AspHI 737 GAGCAC G[AT]GC[AT]^C
3465 GTGCAC
BbsI 2282 GTCTTC GAAGAC(2/6)
4872 GTCTTC
BbvCI 3555 CCTCAGC CCTCAGC(-5/-2)
1294 GCTGAGG
BcgI 1347 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4212 GCAGCCGCCTCG
BlpI 413 GCTAAGC GC^T[ACGT]AGC
934 GCTGAGC
Bpu1102I 413 GCTAAGC GC^T[ACGT]AGC
934 GCTGAGC
BpuAI 2282 GTCTTC GAAGAC(2/6)
4872 GTCTTC
BsaI 581 GAGACC GGTCTC(1/5)
2430 GGTCTC
BsiHKAI 737 GAGCAC G[AT]GC[AT]^C
3465 GTGCAC
Bsp1407I 369 TGTACA T^GTACA
4665 TGTACA
BspHI 3067 TCATGA T^CATGA
3481 TCATGA
BspMI 1158 ACCTGC ACCTGC(4/8)
1634 ACCTGC
BsrGI 369 TGTACA T^GTACA
4665 TGTACA
CelII 413 GCTAAGC GC^T[ACGT]AGC
934 GCTGAGC
DraI 2811 TTTAAA TTT^AAA
4689 TTTAAA
DrdI 709 GACGCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3288 GACCTGACTGTC
Eam1105I 773 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1606 GACAGTGCGTC
NcoI 1733 CCATGG C^CATGG
1757 CCATGG
RcaI 3067 TCATGA T^CATGA
3481 TCATGA
SapI 1707 GCTCTTC GCTCTTC(1/4)
2549 GCTCTTC
SspBI 369 TGTACA T^GTACA
4665 TGTACA
SspI 2663 AATATT AAT^ATT
2806 AATATT
TaqI 639 TCGA T^CGA
2233 TCGA
TfiI 2686 GAATC G^A[AT]TC
2859 GATTC
TthHB8I 639 TCGA T^CGA
2233 TCGA
XmnI 2686 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4095 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI