##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII BamHI BciVI BclI BfrI
BglII BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 126 TCCGGA T^CCGGA
AflIII 5053 ACGTGT A^C[GA][CT]GT
AhdI 4684 GACCGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3401 GTGCAC G^TGCAC
AocI 3487 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1844 AGCGCT AGC^GCT
ApaLI 3401 GTGCAC G^TGCAC
AspEI 4684 GACCGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 872 CCTAGG C^CTAGG
BlnI 872 CCTAGG C^CTAGG
BsaWI 126 TCCGGA [AT]^CCGG[AT]
BseAI 126 TCCGGA T^CCGGA
BsiEI 109 CGACCG CG[GA][CT]^CG
BspEI 126 TCCGGA T^CCGGA
Bsu36I 3487 CCTCAGG CC^T[ACGT]AGG
DrdI 818 GACCAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4684 GACCGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1844 AGCGCT AGC^GCT
Eco81I 3487 CCTCAGG CC^T[ACGT]AGG
KspI 4963 CCGCGG CCGC^GG
MroI 126 TCCGGA T^CCGGA
NspI 834 GCATGT [GA]CATG^[CT]
PshAI 3557 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4963 CCGCGG CCGC^GG
SapI 4304 GAAGAGC GCTCTTC(1/4)
SbfI 5111 CCTGCAGG CCTGCA^GG
SfiI 1870 GGCCTACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 5111 CCTGCAGG CCTGCA^GG
SspI 5460 AATATT AAT^ATT
TfiI 487 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AatII 1023 GACGTC GACGT^C
5067 GACGTC
AcsI 2053 GAATTC [GA]^AATT[CT]
4556 GAATTC
ApoI 2053 GAATTC [GA]^AATT[CT]
4556 GAATTC
AspI 27 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1020 GACGACGTC
BbsI 2710 GAAGAC GAAGAC(2/6)
4562 GTCTTC
BcgI 1582 CGAGGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1146 GCAGATGAGTCG
BpuAI 2710 GAAGAC GAAGAC(2/6)
4562 GTCTTC
BsrBI 1808 CCGCTC CCGCTC(-3/-3)
4789 GAGCGG
EcoRI 2053 GAATTC G^AATTC
4556 GAATTC
HindIII 807 AAGCTT A^AGCTT
3074 AAGCTT
NheI 2559 GCTAGC G^CTAGC
3968 GCTAGC
PflFI 27 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1020 GACGACGTC
Tth111I 27 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1020 GACGACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PaeR7I PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI