##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccI AcsI AflII AgeI
ApoI AscI AseI AsnI Asp718I
BamHI BciVI BfrI BsaBI BsiEI
BsiWI Bsp1407I BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DraI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FseI HindIII HpaI KpnI MamI
MfeI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4884 GACGTC GACGT^C
AccIII 4683 TCCGGA T^CCGGA
AclI 4794 AACGTT AA^CGTT
AhdI 3484 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4075 GTGCAC G^TGCAC
ApaLI 4075 GTGCAC G^TGCAC
AspEI 3484 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5197 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4006 TGCGCA TGC^GCA
BbrPI 4070 CACGTG CAC^GTG
BbsI 3341 GAAGAC GAAGAC(2/6)
BcgI 677 GCACGTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4546 TGATCA T^GATCA
BglII 4641 AGATCT A^GATCT
BpuAI 3341 GAAGAC GAAGAC(2/6)
BseAI 4683 TCCGGA T^CCGGA
BspDI 4485 ATCGAT AT^CGAT
BspEI 4683 TCCGGA T^CCGGA
BspLU11I 4890 ACATGT A^CATGT
BstEII 4383 GGTGACC G^GT[ACGT]ACC
BstPI 4383 GGTGACC G^GT[ACGT]ACC
ClaI 4485 ATCGAT AT^CGAT
Eam1105I 3484 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4383 GGTGACC G^GT[ACGT]ACC
EcoT22I 3593 ATGCAT ATGCA^T
FbaI 4546 TGATCA T^GATCA
FspI 4006 TGCGCA TGC^GCA
MluI 4443 ACGCGT A^CGCGT
MroI 4683 TCCGGA T^CCGGA
NsiI 3593 ATGCAT ATGCA^T
PflFI 5197 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 740 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4070 CACGTG CAC^GTG
PmlI 4070 CACGTG CAC^GTG
PshAI 1871 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4794 AACGTT AA^CGTT
SapI 2992 GCTCTTC GCTCTTC(1/4)
SmlI 3756 CTCAAG C^T[CT][GA]AG
Tsp509I 3765 AATT ^AATT
Tth111I 5197 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 740 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 1565 CCTGAGG CC^T[ACGT]AGG
3450 CCTCAGG
Aor51HI 2459 AGCGCT AGC^GCT
3611 AGCGCT
Asp700I 3269 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3341 GAAGACCTTC
BbeI 1954 GGCGCC GGCGC^C
3994 GGCGCC
BsaAI 4070 CACGTG [CT]AC^GT[GA]
4818 CACGTA
BsaWI 2241 TCCGGT [AT]^CCGG[AT]
4683 TCCGGA
BspHI 2464 TCATGA T^CATGA
2743 TCATGA
BsrDI 1799 CATTGC GCAATG(2/0)
4523 CATTGC
BstXI 450 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3328 CCAAGGCTGTGG
BstYI 3019 GGATCT [GA]^GATC[CT]
4641 AGATCT
Bsu36I 1565 CCTGAGG CC^T[ACGT]AGG
3450 CCTCAGG
Eco47III 2459 AGCGCT AGC^GCT
3611 AGCGCT
Eco81I 1565 CCTGAGG CC^T[ACGT]AGG
3450 CCTCAGG
HincII 189 GTCAAC GT[CT]^[GA]AC
2871 GTCAAC
HindII 189 GTCAAC GT[CT]^[GA]AC
2871 GTCAAC
KasI 1954 GGCGCC G^GCGCC
3994 GGCGCC
KspI 1881 CCGCGG CCGC^GG
4598 CCGCGG
MflI 3019 GGATCT [GA]^GATC[CT]
4641 AGATCT
NaeI 2853 GCCGGC GCC^GGC
4293 GCCGGC
NarI 1954 GGCGCC GG^CGCC
3994 GGCGCC
NgoMIV 2853 GCCGGC G^CCGGC
4293 GCCGGC
RcaI 2464 TCATGA T^CATGA
2743 TCATGA
SacII 1881 CCGCGG CCGC^GG
4598 CCGCGG
SexAI 2689 ACCTGGT A^CC[AT]GGT
2881 ACCAGGT
SfiI 3524 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3818 GGCCGGTGGGGCC
SfoI 1954 GGCGCC GGC^GCC
3994 GGCGCC
SphI 1976 GCATGC GCATG^C
5229 GCATGC
XhoII 3019 GGATCT [GA]^GATC[CT]
4641 AGATCT
XmnI 3269 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3341 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI