##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AccIII AgeI AhdI AscI
AspEI AviII BbrPI BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssSI Bst1107I BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI FseI FspI MamI
MfeI MluI MroI MunI NaeI
NcoI NgoMIV NotI NruI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SalI SbfI ScaI
SgrAI SnaBI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 4381 GACGTC GACGT^C
AclI 74 AACGTT AA^CGTT
AflII 2731 CTTAAG C^TTAAG
AflIII 2671 ACATGT A^C[GA][CT]GT
Alw44I 820 GTGCAC G^TGCAC
ApaLI 820 GTGCAC G^TGCAC
AseI 2656 ATTAAT AT^TAAT
AsnI 2656 ATTAAT AT^TAAT
AvrII 831 CCTAGG C^CTAGG
BalI 1915 TGGCCA TGG^CCA
BamHI 4392 GGATCC G^GATCC
BcgI 1810 CGATTCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 505 GGATAC GTATCC(6/5)
BclI 5055 TGATCA T^GATCA
BfrI 2731 CTTAAG C^TTAAG
BlnI 831 CCTAGG C^CTAGG
BsaWI 564 TCCGGT [AT]^CCGG[AT]
BspHI 4349 TCATGA T^CATGA
BspLU11I 2671 ACATGT A^CATGT
BssHII 1851 GCGCGC G^CGCGC
BstBI 5038 TTCGAA TT^CGAA
DraI 1712 TTTAAA TTT^AAA
EcoRV 3391 GATATC GAT^ATC
EcoT22I 638 ATGCAT ATGCA^T
FbaI 5055 TGATCA T^GATCA
HincII 4193 GTTAAC GT[CT]^[GA]AC
HindII 4193 GTTAAC GT[CT]^[GA]AC
HindIII 3961 AAGCTT A^AGCTT
HpaI 4193 GTTAAC GTT^AAC
KspI 3386 CCGCGG CCGC^GG
MluNI 1915 TGGCCA TGG^CCA
MscI 1915 TGGCCA TGG^CCA
NdeI 2766 CATATG CA^TATG
NheI 4496 GCTAGC G^CTAGC
NsiI 638 ATGCAT ATGCA^T
NspV 5038 TTCGAA TT^CGAA
PacI 2653 TTAATTAA TTAAT^TAA
PshBI 2656 ATTAAT AT^TAAT
Psp1406I 74 AACGTT AA^CGTT
RcaI 4349 TCATGA T^CATGA
SacII 3386 CCGCGG CCGC^GG
SexAI 260 ACCTGGT A^CC[AT]GGT
SfiI 1217 GGCCTCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5038 TTCGAA TT^CGAA
SmaI 3830 CCCGGG CCC^GGG
SpeI 4912 ACTAGT A^CTAGT
SphI 2880 GCATGC GCATG^C
SspI 2798 AATATT AAT^ATT
XmaCI 3830 CCCGGG C^CCGGG
XmaI 3830 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 257 GGTACC G^GTACC
3292 GGTACC
ApaI 2083 GGGCCC GGGCC^C
4680 GGGCCC
Asp700I 8 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3154 GAACCTGTTC
Asp718I 257 GGTACC G^GTACC
3292 GGTACC
AvaI 1533 CCCGAG C^[CT]CG[GA]G
3830 CCCGGG
BbeI 3589 GGCGCC GGCGC^C
4620 GGCGCC
BbsI 3086 GAAGAC GAAGAC(2/6)
3848 GTCTTC
BbvCI 1657 CCTCAGC CCTCAGC(-5/-2)
1963 CCTCAGC
BglII 971 AGATCT A^GATCT
4773 AGATCT
BpuAI 3086 GAAGAC GAAGAC(2/6)
3848 GTCTTC
BsmBI 4266 CGTCTC CGTCTC(1/5)
109 GAGACG
BsmI 2810 GCATTC GAATGC(1/-1)
5035 GCATTC
BsoBI 1533 CCCGAG C^[CT]CG[GA]G
3830 CCCGGG
BsrDI 168 CATTGC GCAATG(2/0)
1367 CATTGC
BstEII 264 GGTGACC G^GT[ACGT]ACC
3109 GGTAACC
BstPI 264 GGTGACC G^GT[ACGT]ACC
3109 GGTAACC
DrdI 4282 GACGGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4339 GACTGCACGGTC
EaeI 1915 TGGCCA [CT]^GGCC[GA]
4158 CGGCCA
EcoO65I 264 GGTGACC G^GT[ACGT]ACC
3109 GGTAACC
HgaI 2918 GCGTC GACGC(5/10)
4265 GCGTC
KasI 3589 GGCGCC G^GCGCC
4620 GGCGCC
KpnI 257 GGTACC GGTAC^C
3292 GGTACC
NarI 3589 GGCGCC GG^CGCC
4620 GGCGCC
SapI 694 GCTCTTC GCTCTTC(1/4)
2561 GCTCTTC
SfoI 3589 GGCGCC GGC^GCC
4620 GGCGCC
XbaI 2192 TCTAGA T^CTAGA
5016 TCTAGA
XcmI 492 CCAGAATGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1068 CCATAGGCTGGGTGG
XmnI 8 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3154 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApoI AspHI AspI AvaII BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EarI Eco47III
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII