##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII Aor51HI ApaI AscI AviII
BbvCI BclI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FbaI FseI FspI KspI
MroI MspA1I NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PmeI PshAI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2337 GACGTC GACGT^C
Acc65I 3798 GGTACC G^GTACC
AccI 3952 GTAGAC GT^[AC][GT]AC
AclI 4378 AACGTT AA^CGTT
AflII 5555 CTTAAG C^TTAAG
AgeI 2859 ACCGGT A^CCGGT
Alw44I 2855 GTGCAC G^TGCAC
AocI 505 CCTCAGG CC^T[ACGT]AGG
ApaLI 2855 GTGCAC G^TGCAC
Asp700I 5643 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3798 GGTACC G^GTACC
AspI 4285 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5376 CCTAGG C^CTAGG
BbeI 5293 GGCGCC GGCGC^C
BbrPI 1602 CACGTG CAC^GTG
BcgI 1342 CGATGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5555 CTTAAG C^TTAAG
BglI 4472 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5376 CCTAGG C^CTAGG
BsaBI 205 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 535 CTGCAC GTGCAG(16/14)
BsiEI 2119 CGACCG CG[GA][CT]^CG
BsmI 1348 GAATGC GAATGC(1/-1)
BspHI 4719 TCATGA T^CATGA
BsrFI 2859 ACCGGT [GA]^CCGG[CT]
BstXI 1655 CCATGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 505 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2859 ACCGGT [GA]^CCGG[CT]
DraIII 1471 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 505 CCTCAGG CC^T[ACGT]AGG
HaeII 5293 GGCGCC [GA]GCGC^[CT]
HindIII 1034 AAGCTT A^AGCTT
KasI 5293 GGCGCC G^GCGCC
KpnI 3798 GGTACC GGTAC^C
MamI 205 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2716 ACGCGT A^CGCGT
NarI 5293 GGCGCC GG^CGCC
PflFI 4285 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2859 ACCGGT A^CCGGT
PmaCI 1602 CACGTG CAC^GTG
PmlI 1602 CACGTG CAC^GTG
Psp1406I 4378 AACGTT AA^CGTT
RcaI 4719 TCATGA T^CATGA
SacI 632 GAGCTC GAGCT^C
SapI 4553 GAAGAGC GCTCTTC(1/4)
ScaI 2166 AGTACT AGT^ACT
SfoI 5293 GGCGCC GGC^GCC
StuI 2311 AGGCCT AGG^CCT
Tth111I 4285 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2557 TCTAGA T^CTAGA
XmnI 5643 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 2337 GACGTC G[GA]^CG[CT]C
5293 GGCGCC
AhdI 389 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1358 GACAGATGGTC
AlwNI 2779 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
4281 CAGTGACTG
AseI 3068 ATTAAT AT^TAAT
3745 ATTAAT
AsnI 3068 ATTAAT AT^TAAT
3745 ATTAAT
AspEI 389 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1358 GACAGATGGTC
BalI 2256 TGGCCA TGG^CCA
5101 TGGCCA
BciVI 743 GGATAC GTATCC(6/5)
3621 GTATCC
BglII 2899 AGATCT A^GATCT
4486 AGATCT
BpmI 977 CTGGAG CTGGAG(16/14)
3425 CTGGAG
BsaAI 1602 CACGTG [CT]AC^GT[GA]
3091 CACGTA
BsaHI 2337 GACGTC G[GA]^CG[CT]C
5293 GGCGCC
BsaWI 614 ACCGGA [AT]^CCGG[AT]
2859 ACCGGT
BseRI 1300 GAGGAG GAGGAG(10/8)
4866 GAGGAG
BsmBI 2339 CGTCTC CGTCTC(1/5)
128 GAGACG
Bsp1407I 1512 TGTACA T^GTACA
4754 TGTACA
BsrDI 4927 CATTGC GCAATG(2/0)
2033 GCAATG
BsrGI 1512 TGTACA T^GTACA
4754 TGTACA
Eam1105I 389 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1358 GACAGATGGTC
EcoRV 2317 GATATC GAT^ATC
4535 GATATC
HgaI 2871 GACGC GACGC(5/10)
2718 GCGTC
Hin1I 2337 GACGTC G[GA]^CG[CT]C
5293 GGCGCC
MluNI 2256 TGGCCA TGG^CCA
5101 TGGCCA
MscI 2256 TGGCCA TGG^CCA
5101 TGGCCA
NcoI 3478 CCATGG C^CATGG
3825 CCATGG
PaeR7I 670 CTCGAG C^TCGAG
1236 CTCGAG
PflMI 5323 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5349 CCAGAGAGTGG
PpuMI 184 GGGTCCC [GA]G^G[AT]CC[CT]
3661 GGGACCC
PshBI 3068 ATTAAT AT^TAAT
3745 ATTAAT
PstI 2690 CTGCAG CTGCA^G
3843 CTGCAG
SmaI 104 CCCGGG CCC^GGG
4639 CCCGGG
SspBI 1512 TGTACA T^GTACA
4754 TGTACA
Van91I 5323 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5349 CCAGAGAGTGG
XhoI 670 CTCGAG C^TCGAG
1236 CTCGAG
XmaCI 104 CCCGGG C^CCGGG
4639 CCCGGG
XmaI 104 CCCGGG C^CCGGG
4639 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspI MunI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII