##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AspI AviII BciVI
BclI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV FbaI FseI FspI
HpaI MamI MfeI MluI MroI
MunI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2678 GACGTC GACGT^C
Acc65I 1321 GGTACC G^GTACC
AcsI 5311 AAATTT [GA]^AATT[CT]
AflII 1491 CTTAAG C^TTAAG
AflIII 2369 ACATGT A^C[GA][CT]GT
Alw44I 2393 GTGCAC G^TGCAC
ApaLI 2393 GTGCAC G^TGCAC
ApoI 5311 AAATTT [GA]^AATT[CT]
AseI 5301 ATTAAT AT^TAAT
AsnI 5301 ATTAAT AT^TAAT
Asp700I 3342 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1321 GGTACC G^GTACC
AvrII 1665 CCTAGG C^CTAGG
BamHI 5190 GGATCC G^GATCC
BbeI 125 GGCGCC GGCGC^C
BbvCI 4353 CCTCAGC CCTCAGC(-5/-2)
BfrI 1491 CTTAAG C^TTAAG
BglI 4374 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1665 CCTAGG C^CTAGG
BsiEI 178 CGGCCG CG[GA][CT]^CG
BsmBI 2680 CGTCTC CGTCTC(1/5)
BsmI 4202 GAATGC GAATGC(1/-1)
Bsp1407I 793 TGTACA T^GTACA
BspLU11I 2369 ACATGT A^CATGT
BsrDI 1242 CATTGC GCAATG(2/0)
BsrGI 793 TGTACA T^GTACA
BstEII 1260 GGTCACC G^GT[ACGT]ACC
BstPI 1260 GGTCACC G^GT[ACGT]ACC
EagI 178 CGGCCG C^GGCCG
EclXI 178 CGGCCG C^GGCCG
Eco52I 178 CGGCCG C^GGCCG
EcoO65I 1260 GGTCACC G^GT[ACGT]ACC
EcoT22I 4204 ATGCAT ATGCA^T
HincII 2529 GTTGAC GT[CT]^[GA]AC
HindII 2529 GTTGAC GT[CT]^[GA]AC
KasI 125 GGCGCC G^GCGCC
KpnI 1321 GGTACC GGTAC^C
NaeI 180 GCCGGC GCC^GGC
NarI 125 GGCGCC GG^CGCC
NdeI 4787 CATATG CA^TATG
NgoMIV 180 GCCGGC G^CCGGC
NheI 862 GCTAGC G^CTAGC
NsiI 4204 ATGCAT ATGCA^T
PshBI 5301 ATTAAT AT^TAAT
SfoI 125 GGCGCC GGC^GCC
SmaI 2078 CCCGGG CCC^GGG
SphI 2993 GCATGC GCATG^C
SspBI 793 TGTACA T^GTACA
SspI 5453 AATATT AAT^ATT
XcmI 4581 CCATGGCCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2078 CCCGGG C^CCGGG
XmaI 2078 CCCGGG C^CCGGG
XmnI 3342 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2940 GTAGAC GT^[AC][GT]AC
4163 GTAGAC
BbrPI 155 CACGTG CAC^GTG
4416 CACGTG
BcgI 505 CGAGATAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2987 CGAGGGGCATGC
BsaAI 155 CACGTG [CT]AC^GT[GA]
4416 CACGTG
DraI 267 TTTAAA TTT^AAA
5457 TTTAAA
DraIII 639 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1157 CACCAGGTG
HaeII 125 GGCGCC [GA]GCGC^[CT]
1079 AGCGCC
HgaI 1369 GCGTC GACGC(5/10)
4358 GCGTC
HindIII 1609 AAGCTT A^AGCTT
3300 AAGCTT
KspI 175 CCGCGG CCGC^GG
3186 CCGCGG
PaeR7I 1119 CTCGAG C^TCGAG
4638 CTCGAG
PmaCI 155 CACGTG CAC^GTG
4416 CACGTG
PmlI 155 CACGTG CAC^GTG
4416 CACGTG
SacII 175 CCGCGG CCGC^GG
3186 CCGCGG
ScaI 96 AGTACT AGT^ACT
4303 AGTACT
SexAI 1158 ACCAGGT A^CC[AT]GGT
1169 ACCAGGT
SpeI 4251 ACTAGT A^CTAGT
4936 ACTAGT
XhoI 1119 CTCGAG C^TCGAG
4638 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII