################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AclI AflII Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AvaI AviII BamHI BbrPI BcgI BciVI BclI BfrI BglI BsiEI BsiWI BsmBI BsoBI BspDI BspMI BsrBI BssHII BssSI Bsu36I ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I FbaI FseI FspI KspI MfeI MunI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 4012 GGTACC G^GTACC AccIII 5069 TCCGGA T^CCGGA AgeI 1050 ACCGGT A^CCGGT AhdI 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 4012 GGTACC G^GTACC AspEI 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1968 TGGCCA TGG^CCA BcnI 5087 CCCGG CC^[GC]GG BlpI 4710 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4710 GCTGAGC GC^T[ACGT]AGC BsaAI 4174 TACGTG [CT]AC^GT[GA] BsaBI 3824 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 5069 TCCGGA T^CCGGA BsgI 3426 GTGCAG GTGCAG(16/14) BspEI 5069 TCCGGA T^CCGGA BstBI 481 TTCGAA TT^CGAA BstEII 3634 GGTAACC G^GT[ACGT]ACC BstPI 3634 GGTAACC G^GT[ACGT]ACC CelII 4710 GCTGAGC GC^T[ACGT]AGC DraIII 3312 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1968 TGGCCA [CT]^GGCC[GA] Eam1105I 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1026 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3634 GGTAACC G^GT[ACGT]ACC EcoRI 5162 GAATTC G^AATTC KpnI 4012 GGTACC GGTAC^C MamI 3824 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 3352 ACGCGT A^CGCGT MluNI 1968 TGGCCA TGG^CCA MroI 5069 TCCGGA T^CCGGA MscI 1968 TGGCCA TGG^CCA NciI 5087 CCCGG CC^[GC]GG NcoI 4919 CCATGG C^CATGG NdeI 5030 CATATG CA^TATG NheI 4581 GCTAGC G^CTAGC NspV 481 TTCGAA TT^CGAA PflFI 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4919 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1050 ACCGGT A^CCGGT PshAI 2795 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 3747 ACCAGGT A^CC[AT]GGT SfuI 481 TTCGAA TT^CGAA SphI 5193 GCATGC GCATG^C Tth111I 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4919 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 4619 GGCGCC G[GA]^CG[CT]C 5044 GGCGCC BanII 4090 GGGCTC G[GA]GC[CT]^C 4816 GAGCCC BbeI 4619 GGCGCC GGCGC^C 5044 GGCGCC BglII 241 AGATCT A^GATCT 5213 AGATCT BsaHI 4619 GGCGCC G[GA]^CG[CT]C 5044 GGCGCC BspHI 631 TCATGA T^CATGA 3331 TCATGA BspLU11I 3029 ACATGT A^CATGT 5389 ACATGT Bst1107I 1759 GTATAC GTA^TAC 2231 GTATAC BstYI 241 AGATCT [GA]^GATC[CT] 5213 AGATCT BstZ17I 1759 GTATAC GTA^TAC 2231 GTATAC DraII 486 AGGCCCT [GA]G^G[ACGT]CC[CT] 3977 AGGCCCC DrdI 3335 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4949 GACGAAAGTGTC DsaI 537 CCGTGG C^C[GA][CT]GG 4919 CCATGG EcoO109I 486 AGGCCCT [GA]G^G[ACGT]CC[CT] 3977 AGGCCCC EcoRV 1994 GATATC GAT^ATC 2106 GATATC EcoT22I 3108 ATGCAT ATGCA^T 3836 ATGCAT FauI 5307 CCCGC CCCGC(4/6) 4929 GCGGG Hin1I 4619 GGCGCC G[GA]^CG[CT]C 5044 GGCGCC HindIII 717 AAGCTT A^AGCTT 920 AAGCTT HpaI 826 GTTAAC GTT^AAC 1231 GTTAAC KasI 4619 GGCGCC G^GCGCC 5044 GGCGCC MaeII 4036 ACGT A^CGT 4175 ACGT MflI 241 AGATCT [GA]^GATC[CT] 5213 AGATCT NaeI 4616 GCCGGC GCC^GGC 4843 GCCGGC NarI 4619 GGCGCC GG^CGCC 5044 GGCGCC NgoMIV 4616 GCCGGC G^CCGGC 4843 GCCGGC NsiI 3108 ATGCAT ATGCA^T 3836 ATGCAT RcaI 631 TCATGA T^CATGA 3331 TCATGA SfoI 4619 GGCGCC GGC^GCC 5044 GGCGCC SspI 1064 AATATT AAT^ATT 1444 AATATT XbaI 622 TCTAGA T^CTAGA 1144 TCTAGA XhoII 241 AGATCT [GA]^GATC[CT] 5213 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII AvrII BanI BbsI BbvCI BbvI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI