##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AflII Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AvaI AviII BamHI BbrPI
BcgI BciVI BclI BfrI BglI
BsiEI BsiWI BsmBI BsoBI BspDI
BspMI BsrBI BssHII BssSI Bsu36I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I FbaI FseI FspI
KspI MfeI MunI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4012 GGTACC G^GTACC
AccIII 5069 TCCGGA T^CCGGA
AgeI 1050 ACCGGT A^CCGGT
AhdI 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4012 GGTACC G^GTACC
AspEI 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1968 TGGCCA TGG^CCA
BcnI 5087 CCCGG CC^[GC]GG
BlpI 4710 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4710 GCTGAGC GC^T[ACGT]AGC
BsaAI 4174 TACGTG [CT]AC^GT[GA]
BsaBI 3824 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5069 TCCGGA T^CCGGA
BsgI 3426 GTGCAG GTGCAG(16/14)
BspEI 5069 TCCGGA T^CCGGA
BstBI 481 TTCGAA TT^CGAA
BstEII 3634 GGTAACC G^GT[ACGT]ACC
BstPI 3634 GGTAACC G^GT[ACGT]ACC
CelII 4710 GCTGAGC GC^T[ACGT]AGC
DraIII 3312 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1968 TGGCCA [CT]^GGCC[GA]
Eam1105I 1974 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1026 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3634 GGTAACC G^GT[ACGT]ACC
EcoRI 5162 GAATTC G^AATTC
KpnI 4012 GGTACC GGTAC^C
MamI 3824 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3352 ACGCGT A^CGCGT
MluNI 1968 TGGCCA TGG^CCA
MroI 5069 TCCGGA T^CCGGA
MscI 1968 TGGCCA TGG^CCA
NciI 5087 CCCGG CC^[GC]GG
NcoI 4919 CCATGG C^CATGG
NdeI 5030 CATATG CA^TATG
NheI 4581 GCTAGC G^CTAGC
NspV 481 TTCGAA TT^CGAA
PflFI 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4919 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1050 ACCGGT A^CCGGT
PshAI 2795 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3747 ACCAGGT A^CC[AT]GGT
SfuI 481 TTCGAA TT^CGAA
SphI 5193 GCATGC GCATG^C
Tth111I 3616 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4919 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 4619 GGCGCC G[GA]^CG[CT]C
5044 GGCGCC
BanII 4090 GGGCTC G[GA]GC[CT]^C
4816 GAGCCC
BbeI 4619 GGCGCC GGCGC^C
5044 GGCGCC
BglII 241 AGATCT A^GATCT
5213 AGATCT
BsaHI 4619 GGCGCC G[GA]^CG[CT]C
5044 GGCGCC
BspHI 631 TCATGA T^CATGA
3331 TCATGA
BspLU11I 3029 ACATGT A^CATGT
5389 ACATGT
Bst1107I 1759 GTATAC GTA^TAC
2231 GTATAC
BstYI 241 AGATCT [GA]^GATC[CT]
5213 AGATCT
BstZ17I 1759 GTATAC GTA^TAC
2231 GTATAC
DraII 486 AGGCCCT [GA]G^G[ACGT]CC[CT]
3977 AGGCCCC
DrdI 3335 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4949 GACGAAAGTGTC
DsaI 537 CCGTGG C^C[GA][CT]GG
4919 CCATGG
EcoO109I 486 AGGCCCT [GA]G^G[ACGT]CC[CT]
3977 AGGCCCC
EcoRV 1994 GATATC GAT^ATC
2106 GATATC
EcoT22I 3108 ATGCAT ATGCA^T
3836 ATGCAT
FauI 5307 CCCGC CCCGC(4/6)
4929 GCGGG
Hin1I 4619 GGCGCC G[GA]^CG[CT]C
5044 GGCGCC
HindIII 717 AAGCTT A^AGCTT
920 AAGCTT
HpaI 826 GTTAAC GTT^AAC
1231 GTTAAC
KasI 4619 GGCGCC G^GCGCC
5044 GGCGCC
MaeII 4036 ACGT A^CGT
4175 ACGT
MflI 241 AGATCT [GA]^GATC[CT]
5213 AGATCT
NaeI 4616 GCCGGC GCC^GGC
4843 GCCGGC
NarI 4619 GGCGCC GG^CGCC
5044 GGCGCC
NgoMIV 4616 GCCGGC G^CCGGC
4843 GCCGGC
NsiI 3108 ATGCAT ATGCA^T
3836 ATGCAT
RcaI 631 TCATGA T^CATGA
3331 TCATGA
SfoI 4619 GGCGCC GGC^GCC
5044 GGCGCC
SspI 1064 AATATT AAT^ATT
1444 AATATT
XbaI 622 TCTAGA T^CTAGA
1144 TCTAGA
XhoII 241 AGATCT [GA]^GATC[CT]
5213 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII AvrII BanI
BbsI BbvCI BbvI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmnI