##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BcnI BglI
BlnI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII BssSI
BstBI BstUI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HindIII KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NspV
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3567 GTATAC GT^[AC][GT]AC
AccIII 427 TCCGGA T^CCGGA
AcyI 1 GGCGTC G[GA]^CG[CT]C
AflII 2492 CTTAAG C^TTAAG
AflIII 4951 ACATGT A^C[GA][CT]GT
ApaI 4199 GGGCCC GGGCC^C
Asp700I 1741 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3763 GGATCC G^GATCC
BfrI 2492 CTTAAG C^TTAAG
BglII 2343 AGATCT A^GATCT
BlpI 1466 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1466 GCTGAGC GC^T[ACGT]AGC
BsaAI 4648 CACGTA [CT]AC^GT[GA]
BsaHI 1 GGCGTC G[GA]^CG[CT]C
BseAI 427 TCCGGA T^CCGGA
BsmI 802 GAATGC GAATGC(1/-1)
BspEI 427 TCCGGA T^CCGGA
BspLU11I 4951 ACATGT A^CATGT
Bst1107I 3567 GTATAC GTA^TAC
BstZ17I 3567 GTATAC GTA^TAC
CelII 1466 GCTGAGC GC^T[ACGT]AGC
DraI 2868 TTTAAA TTT^AAA
EcoRI 5414 GAATTC G^AATTC
EcoT22I 906 ATGCAT ATGCA^T
Hin1I 1 GGCGTC G[GA]^CG[CT]C
HpaI 2531 GTTAAC GTT^AAC
MroI 427 TCCGGA T^CCGGA
NaeI 1356 GCCGGC GCC^GGC
NgoMIV 1356 GCCGGC G^CCGGC
NsiI 906 ATGCAT ATGCA^T
PstI 4521 CTGCAG CTGCA^G
SacI 1469 GAGCTC GAGCT^C
SphI 1870 GCATGC GCATG^C
StuI 4706 AGGCCT AGG^CCT
XbaI 4126 TCTAGA T^CTAGA
XmnI 1741 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 1248 TGGCCA TGG^CCA
4607 TGGCCA
BbvCI 3283 GCTGAGG CCTCAGC(-5/-2)
4022 GCTGAGG
BsmBI 3 CGTCTC CGTCTC(1/5)
1700 GAGACG
BspHI 571 TCATGA T^CATGA
3165 TCATGA
BsrDI 909 CATTGC GCAATG(2/0)
2511 CATTGC
BstEII 2297 GGTTACC G^GT[ACGT]ACC
3501 GGTGACC
BstPI 2297 GGTTACC G^GT[ACGT]ACC
3501 GGTGACC
DsaI 1692 CCACGG C^C[GA][CT]GG
5238 CCGTGG
EcoO65I 2297 GGTTACC G^GT[ACGT]ACC
3501 GGTGACC
EcoRV 1850 GATATC GAT^ATC
4785 GATATC
HaeII 1148 AGCGCC [GA]GCGC^[CT]
4061 GGCGCT
HincII 2531 GTTAAC GT[CT]^[GA]AC
3706 GTCAAC
HindII 2531 GTTAAC GT[CT]^[GA]AC
3706 GTCAAC
MaeII 4649 ACGT A^CGT
4988 ACGT
MluNI 1248 TGGCCA TGG^CCA
4607 TGGCCA
MscI 1248 TGGCCA TGG^CCA
4607 TGGCCA
PacI 1370 TTAATTAA TTAAT^TAA
3430 TTAATTAA
PaeR7I 1206 CTCGAG C^TCGAG
4690 CTCGAG
PflMI 3012 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4593 CCAGTGTTTGG
RcaI 571 TCATGA T^CATGA
3165 TCATGA
SapI 3616 GAAGAGC GCTCTTC(1/4)
4437 GAAGAGC
SexAI 456 ACCTGGT A^CC[AT]GGT
2004 ACCAGGT
SspI 2556 AATATT AAT^ATT
3398 AATATT
Van91I 3012 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4593 CCAGTGTTTGG
XhoI 1206 CTCGAG C^TCGAG
4690 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BciVI BclI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII