##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI Aor51HI ApaI AscI AvaI
AviII BamHI BbeI BbrPI BcgI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I FseI FspI HaeII
Hin1I KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1360 GGTACC G^GTACC
AccI 4238 GTAGAC GT^[AC][GT]AC
AccII 4708 CGCG CG^CG
AhdI 2586 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 559 ATTAAT AT^TAAT
AsnI 559 ATTAAT AT^TAAT
Asp700I 2821 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1360 GGTACC G^GTACC
AspEI 2586 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 3757 TGATCA T^GATCA
BcnI 3680 CCGGG CC^[GC]GG
BseRI 1200 GAGGAG GAGGAG(10/8)
BspMI 1409 GCAGGT ACCTGC(4/8)
BssSI 3061 CACGAG CACGAG(-5/-1)
BstEII 2621 GGTTACC G^GT[ACGT]ACC
BstPI 2621 GGTTACC G^GT[ACGT]ACC
BstUI 4708 CGCG CG^CG
CfoI 4707 GCGC GCG^C
DraII 5217 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 5395 GACAGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2586 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1576 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 5217 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2621 GGTTACC G^GT[ACGT]ACC
EcoRI 2907 GAATTC G^AATTC
EcoRV 2763 GATATC GAT^ATC
FbaI 3757 TGATCA T^GATCA
HgaI 4873 GCGTC GACGC(5/10)
HhaI 4707 GCGC GCG^C
HinP1I 4707 GCGC G^CGC
HindIII 722 AAGCTT A^AGCTT
KpnI 1360 GGTACC GGTAC^C
MvnI 4708 CGCG CG^CG
NciI 3680 CCGGG CC^[GC]GG
PacI 556 TTAATTAA TTAAT^TAA
PflMI 4155 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 559 ATTAAT AT^TAAT
SexAI 1493 ACCTGGT A^CC[AT]GGT
SspI 3490 AATATT AAT^ATT
StuI 5068 AGGCCT AGG^CCT
Van91I 4155 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2947 CCATCTCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2821 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 153 ACATGT A^C[GA][CT]GT
4486 ACATGT
Alw44I 2103 GTGCAC G^TGCAC
4615 GTGCAC
AlwNI 3708 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
5288 CAGAGACTG
ApaLI 2103 GTGCAC G^TGCAC
4615 GTGCAC
AvrII 1581 CCTAGG C^CTAGG
2165 CCTAGG
BalI 1212 TGGCCA TGG^CCA
1622 TGGCCA
BglII 5058 AGATCT A^GATCT
5304 AGATCT
BlnI 1581 CCTAGG C^CTAGG
2165 CCTAGG
BsmBI 3064 GAGACG CGTCTC(1/5)
4103 GAGACG
BspLU11I 153 ACATGT A^CATGT
4486 ACATGT
DsaI 3396 CCATGG C^C[GA][CT]GG
5161 CCATGG
EaeI 1212 TGGCCA [CT]^GGCC[GA]
1622 TGGCCA
FauI 3930 CCCGC CCCGC(4/6)
4299 CCCGC
HapII 2041 CCGG C^CGG
3680 CCGG
HincII 600 GTTAAC GT[CT]^[GA]AC
2738 GTTAAC
HindII 600 GTTAAC GT[CT]^[GA]AC
2738 GTTAAC
HpaI 600 GTTAAC GTT^AAC
2738 GTTAAC
HpaII 2041 CCGG C^CGG
3680 CCGG
MluNI 1212 TGGCCA TGG^CCA
1622 TGGCCA
MscI 1212 TGGCCA TGG^CCA
1622 TGGCCA
MspA1I 3174 CAGCTG C[AC]G^C[GT]G
4727 CAGCTG
MspI 2041 CCGG C^CGG
3680 CCGG
NcoI 3396 CCATGG C^CATGG
5161 CCATGG
NdeI 1099 CATATG CA^TATG
5101 CATATG
PstI 3770 CTGCAG CTGCA^G
4639 CTGCAG
PvuII 3174 CAGCTG CAG^CTG
4727 CAGCTG
SpeI 1379 ACTAGT A^CTAGT
1830 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AspHI AspI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII