##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AgeI AocI
AscI AseI AsnI BcgI BciVI
BclI BfrI BseAI BsiWI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraIII Eco81I EcoO65I EcoRV
FbaI FseI MroI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI SapI
SexAI SfuI SgrAI SnaBI SpeI
SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3 GACGTC GACGT^C
Acc65I 1013 GGTACC G^GTACC
Aor51HI 2685 AGCGCT AGC^GCT
Asp718I 1013 GGTACC G^GTACC
AviII 1037 TGCGCA TGC^GCA
AvrII 4747 CCTAGG C^CTAGG
BamHI 1395 GGATCC G^GATCC
BglII 3167 AGATCT A^GATCT
BlnI 4747 CCTAGG C^CTAGG
BlpI 1251 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1251 GCTGAGC GC^T[ACGT]AGC
BsaBI 1582 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2577 GAGACC GGTCTC(1/5)
BsaWI 2358 ACCGGA [AT]^CCGG[AT]
BsiEI 4709 CGGCCG CG[GA][CT]^CG
BsmBI 4739 CGTCTC CGTCTC(1/5)
BsmI 2424 GAATGC GAATGC(1/-1)
Bsp1407I 4805 TGTACA T^GTACA
BsrFI 340 GCCGGC [GA]^CCGG[CT]
BsrGI 4805 TGTACA T^GTACA
CelII 1251 GCTGAGC GC^T[ACGT]AGC
Cfr10I 340 GCCGGC [GA]^CCGG[CT]
CpoI 443 CGGACCG CG^G[AT]CCG
DraI 1150 TTTAAA TTT^AAA
EagI 4709 CGGCCG C^GGCCG
EclXI 4709 CGGCCG C^GGCCG
Eco47III 2685 AGCGCT AGC^GCT
Eco52I 4709 CGGCCG C^GGCCG
EcoRI 3046 GAATTC G^AATTC
EcoT22I 2174 ATGCAT ATGCA^T
FspI 1037 TGCGCA TGC^GCA
KpnI 1013 GGTACC GGTAC^C
KspI 4600 CCGCGG CCGC^GG
MamI 1582 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4157 CAATTG C^AATTG
MluI 3259 ACGCGT A^CGCGT
MunI 4157 CAATTG C^AATTG
NaeI 340 GCCGGC GCC^GGC
NdeI 3628 CATATG CA^TATG
NgoMIV 340 GCCGGC G^CCGGC
NsiI 2174 ATGCAT ATGCA^T
PaeR7I 644 CTCGAG C^TCGAG
PpuMI 605 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1306 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 443 CGGACCG CG^G[AT]CCG
SacI 580 GAGCTC GAGCT^C
SacII 4600 CCGCGG CCGC^GG
SbfI 723 CCTGCAGG CCTGCA^GG
Sse8387I 723 CCTGCAGG CCTGCA^GG
SspBI 4805 TGTACA T^GTACA
XhoI 644 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 3925 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4012 GACGAAAAGTC
Alw44I 42 GTGCAC G^TGCAC
368 GTGCAC
ApaLI 42 GTGCAC G^TGCAC
368 GTGCAC
AspEI 3925 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4012 GACGAAAAGTC
BbeI 778 GGCGCC GGCGC^C
4412 GGCGCC
BbrPI 1329 CACGTG CAC^GTG
4649 CACGTG
BbsI 1135 GAAGAC GAAGAC(2/6)
104 GTCTTC
BpuAI 1135 GAAGAC GAAGAC(2/6)
104 GTCTTC
BsaAI 1329 CACGTG [CT]AC^GT[GA]
4649 CACGTG
BspLU11I 1235 ACATGT A^CATGT
2034 ACATGT
BstAPI 2746 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3020 GCAAGTACTGC
BstXI 2663 CCACGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4418 CCAGAGCCGTGG
DrdI 1081 GACGCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1345 GACCCCCCCGTC
Eam1105I 3925 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4012 GACGAAAAGTC
HindIII 1916 AAGCTT A^AGCTT
3112 AAGCTT
HpaI 1128 GTTAAC GTT^AAC
1179 GTTAAC
KasI 778 GGCGCC G^GCGCC
4412 GGCGCC
NarI 778 GGCGCC GG^CGCC
4412 GGCGCC
NcoI 182 CCATGG C^CATGG
2028 CCATGG
PflMI 546 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCAGCGTCTGG
PmaCI 1329 CACGTG CAC^GTG
4649 CACGTG
PmlI 1329 CACGTG CAC^GTG
4649 CACGTG
ScaI 3023 AGTACT AGT^ACT
4901 AGTACT
SfiI 721 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4604 GGCCTCGGAGGCC
SfoI 778 GGCGCC GGC^GCC
4412 GGCGCC
SmaI 3335 CCCGGG CCC^GGG
3890 CCCGGG
SphI 394 GCATGC GCATG^C
1256 GCATGC
Van91I 546 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCAGCGTCTGG
XmaCI 3335 CCCGGG C^CCGGG
3890 CCCGGG
XmaI 3335 CCCGGG C^CCGGG
3890 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PstI PvuII RsaI SalI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmnI