################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccIII AgeI AhdI Aor51HI AscI AspEI AspI AvaI AvrII BamHI BbrPI BcgI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspEI BsrBI BsrFI BssSI BstAPI BstBI CelII Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI HgaI KspI MluI MroI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 5179 AACGTT AA^CGTT Alw44I 4231 GTGCAC G^TGCAC AlwNI 2597 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 4231 GTGCAC G^TGCAC AviII 3458 TGCGCA TGC^GCA BalI 4577 TGGCCA TGG^CCA BbvCI 17 CCTCAGC CCTCAGC(-5/-2) BciVI 282 GTATCC GTATCC(6/5) BcnI 2281 CCGGG CC^[GC]GG BglII 4441 AGATCT A^GATCT BsaAI 1458 TACGTG [CT]AC^GT[GA] BsaI 2621 GAGACC GGTCTC(1/5) Bsp1407I 4309 TGTACA T^GTACA BspDI 363 ATCGAT AT^CGAT BsrGI 4309 TGTACA T^GTACA Bst1107I 5153 GTATAC GTA^TAC BstEII 2527 GGTAACC G^GT[ACGT]ACC BstPI 2527 GGTAACC G^GT[ACGT]ACC BstZ17I 5153 GTATAC GTA^TAC ClaI 363 ATCGAT AT^CGAT EaeI 4577 TGGCCA [CT]^GGCC[GA] EcoO65I 2527 GGTAACC G^GT[ACGT]ACC EcoRI 3099 GAATTC G^AATTC FspI 3458 TGCGCA TGC^GCA MfeI 864 CAATTG C^AATTG MluNI 4577 TGGCCA TGG^CCA MscI 4577 TGGCCA TGG^CCA MunI 864 CAATTG C^AATTG NciI 2281 CCGGG CC^[GC]GG PflMI 473 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 5179 AACGTT AA^CGTT SacI 419 GAGCTC GAGCT^C SpeI 4935 ACTAGT A^CTAGT SspBI 4309 TGTACA T^GTACA Van91I 473 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 195 GGCGCC G[GA]^CG[CT]C 249 GGCGCC ApaI 239 GGGCCC GGGCC^C 268 GGGCCC AseI 2175 ATTAAT AT^TAAT 3256 ATTAAT AsnI 2175 ATTAAT AT^TAAT 3256 ATTAAT BbeI 195 GGCGCC GGCGC^C 249 GGCGCC BbsI 3142 GAAGAC GAAGAC(2/6) 166 GTCTTC BpuAI 3142 GAAGAC GAAGAC(2/6) 166 GTCTTC BsaBI 339 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5648 GATATTGATC BsaHI 195 GGCGCC G[GA]^CG[CT]C 249 GGCGCC BsaWI 292 TCCGGT [AT]^CCGG[AT] 4918 TCCGGT BspLU11I 4331 ACATGT A^CATGT 5149 ACATGT BsrDI 1867 CATTGC GCAATG(2/0) 3902 CATTGC BssHII 107 GCGCGC G^CGCGC 109 GCGCGC EcoNI 1070 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3031 CCTTTAGTAGG EcoT22I 2864 ATGCAT ATGCA^T 4429 ATGCAT HaeII 195 GGCGCC [GA]GCGC^[CT] 249 GGCGCC Hin1I 195 GGCGCC G[GA]^CG[CT]C 249 GGCGCC HindIII 328 AAGCTT A^AGCTT 903 AAGCTT KasI 195 GGCGCC G^GCGCC 249 GGCGCC MamI 339 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5648 GATATTGATC MspA1I 573 CAGCTG C[AC]G^C[GT]G 588 CAGCTG NarI 195 GGCGCC GG^CGCC 249 GGCGCC NcoI 1235 CCATGG C^CATGG 4580 CCATGG NdeI 3789 CATATG CA^TATG 5052 CATATG NsiI 2864 ATGCAT ATGCA^T 4429 ATGCAT PpuMI 117 GGGTCCC [GA]G^G[AT]CC[CT] 887 GGGACCT PshBI 2175 ATTAAT AT^TAAT 3256 ATTAAT PvuII 573 CAGCTG CAG^CTG 588 CAGCTG SfoI 195 GGCGCC GGC^GCC 249 GGCGCC StuI 1091 AGGCCT AGG^CCT 4359 AGGCCT XbaI 2170 TCTAGA T^CTAGA 3116 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AflII AflIII AluI AlwI AocI ApoI Asp700I Asp718I AspHI AvaII BanI BanII BbvI BclI BfaI BfrI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI