##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AspI AvaI AvrII
BamHI BbrPI BcgI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspEI BsrBI BsrFI
BssSI BstAPI BstBI CelII Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
HgaI KspI MluI MroI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 5179 AACGTT AA^CGTT
Alw44I 4231 GTGCAC G^TGCAC
AlwNI 2597 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4231 GTGCAC G^TGCAC
AviII 3458 TGCGCA TGC^GCA
BalI 4577 TGGCCA TGG^CCA
BbvCI 17 CCTCAGC CCTCAGC(-5/-2)
BciVI 282 GTATCC GTATCC(6/5)
BcnI 2281 CCGGG CC^[GC]GG
BglII 4441 AGATCT A^GATCT
BsaAI 1458 TACGTG [CT]AC^GT[GA]
BsaI 2621 GAGACC GGTCTC(1/5)
Bsp1407I 4309 TGTACA T^GTACA
BspDI 363 ATCGAT AT^CGAT
BsrGI 4309 TGTACA T^GTACA
Bst1107I 5153 GTATAC GTA^TAC
BstEII 2527 GGTAACC G^GT[ACGT]ACC
BstPI 2527 GGTAACC G^GT[ACGT]ACC
BstZ17I 5153 GTATAC GTA^TAC
ClaI 363 ATCGAT AT^CGAT
EaeI 4577 TGGCCA [CT]^GGCC[GA]
EcoO65I 2527 GGTAACC G^GT[ACGT]ACC
EcoRI 3099 GAATTC G^AATTC
FspI 3458 TGCGCA TGC^GCA
MfeI 864 CAATTG C^AATTG
MluNI 4577 TGGCCA TGG^CCA
MscI 4577 TGGCCA TGG^CCA
MunI 864 CAATTG C^AATTG
NciI 2281 CCGGG CC^[GC]GG
PflMI 473 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 5179 AACGTT AA^CGTT
SacI 419 GAGCTC GAGCT^C
SpeI 4935 ACTAGT A^CTAGT
SspBI 4309 TGTACA T^GTACA
Van91I 473 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 195 GGCGCC G[GA]^CG[CT]C
249 GGCGCC
ApaI 239 GGGCCC GGGCC^C
268 GGGCCC
AseI 2175 ATTAAT AT^TAAT
3256 ATTAAT
AsnI 2175 ATTAAT AT^TAAT
3256 ATTAAT
BbeI 195 GGCGCC GGCGC^C
249 GGCGCC
BbsI 3142 GAAGAC GAAGAC(2/6)
166 GTCTTC
BpuAI 3142 GAAGAC GAAGAC(2/6)
166 GTCTTC
BsaBI 339 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5648 GATATTGATC
BsaHI 195 GGCGCC G[GA]^CG[CT]C
249 GGCGCC
BsaWI 292 TCCGGT [AT]^CCGG[AT]
4918 TCCGGT
BspLU11I 4331 ACATGT A^CATGT
5149 ACATGT
BsrDI 1867 CATTGC GCAATG(2/0)
3902 CATTGC
BssHII 107 GCGCGC G^CGCGC
109 GCGCGC
EcoNI 1070 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3031 CCTTTAGTAGG
EcoT22I 2864 ATGCAT ATGCA^T
4429 ATGCAT
HaeII 195 GGCGCC [GA]GCGC^[CT]
249 GGCGCC
Hin1I 195 GGCGCC G[GA]^CG[CT]C
249 GGCGCC
HindIII 328 AAGCTT A^AGCTT
903 AAGCTT
KasI 195 GGCGCC G^GCGCC
249 GGCGCC
MamI 339 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5648 GATATTGATC
MspA1I 573 CAGCTG C[AC]G^C[GT]G
588 CAGCTG
NarI 195 GGCGCC GG^CGCC
249 GGCGCC
NcoI 1235 CCATGG C^CATGG
4580 CCATGG
NdeI 3789 CATATG CA^TATG
5052 CATATG
NsiI 2864 ATGCAT ATGCA^T
4429 ATGCAT
PpuMI 117 GGGTCCC [GA]G^G[AT]CC[CT]
887 GGGACCT
PshBI 2175 ATTAAT AT^TAAT
3256 ATTAAT
PvuII 573 CAGCTG CAG^CTG
588 CAGCTG
SfoI 195 GGCGCC GGC^GCC
249 GGCGCC
StuI 1091 AGGCCT AGG^CCT
4359 AGGCCT
XbaI 2170 TCTAGA T^CTAGA
3116 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflII AflIII AluI AlwI
AocI ApoI Asp700I Asp718I AspHI
AvaII BanI BanII BbvI BclI
BfaI BfrI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI