##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AgeI AhdI AscI
AspEI AviII AvrII BalI BamHI
BbrPI BlnI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eam1105I EcoO65I
EcoRV FseI FspI KspI MamI
MluI MluNI MroI MscI NcoI
NheI NotI NruI NspV PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 580 GGTACC G^GTACC
AclI 945 AACGTT AA^CGTT
AflII 592 CTTAAG C^TTAAG
AocI 2883 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3589 AGCGCT AGC^GCT
Asp718I 580 GGTACC G^GTACC
BcgI 1751 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 96 GGATAC GTATCC(6/5)
BfrI 592 CTTAAG C^TTAAG
BglI 4569 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2804 AGATCT A^GATCT
BlpI 364 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 364 GCTCAGC GC^T[ACGT]AGC
BsaAI 4466 TACGTG [CT]AC^GT[GA]
BsrBI 145 GAGCGG CCGCTC(-3/-3)
BsrFI 4716 GCCGGC [GA]^CCGG[CT]
BssSI 1626 CACGAG CACGAG(-5/-1)
BstAPI 4578 GCAGGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2883 CCTAAGG CC^T[ACGT]AGG
CelII 364 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4716 GCCGGC [GA]^CCGG[CT]
DraIII 86 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1285 CCGTGG C^C[GA][CT]GG
EagI 528 CGGCCG C^GGCCG
EclXI 528 CGGCCG C^GGCCG
Eco47III 3589 AGCGCT AGC^GCT
Eco52I 528 CGGCCG C^GGCCG
Eco81I 2883 CCTAAGG CC^T[ACGT]AGG
EcoT22I 855 ATGCAT ATGCA^T
KpnI 580 GGTACC GGTAC^C
NaeI 4716 GCCGGC GCC^GGC
NdeI 3969 CATATG CA^TATG
NgoMIV 4716 GCCGGC G^CCGGC
NsiI 855 ATGCAT ATGCA^T
Psp1406I 945 AACGTT AA^CGTT
PvuII 4632 CAGCTG CAG^CTG
SacI 703 GAGCTC GAGCT^C
SphI 2588 GCATGC GCATG^C
StuI 1294 AGGCCT AGG^CCT
SwaI 1501 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 189 GGCGCC G[GA]^CG[CT]C
1717 GGCGCC
Alw44I 2534 GTGCAC G^TGCAC
4637 GTGCAC
ApaI 643 GGGCCC GGGCC^C
1569 GGGCCC
ApaLI 2534 GTGCAC G^TGCAC
4637 GTGCAC
AseI 3721 ATTAAT AT^TAAT
4067 ATTAAT
AsnI 3721 ATTAAT AT^TAAT
4067 ATTAAT
BbeI 189 GGCGCC GGCGC^C
1717 GGCGCC
BclI 1540 TGATCA T^GATCA
4183 TGATCA
BsaHI 189 GGCGCC G[GA]^CG[CT]C
1717 GGCGCC
BsaI 1342 GAGACC GGTCTC(1/5)
1643 GAGACC
BsiEI 528 CGGCCG CG[GA][CT]^CG
5171 CGGTCG
Bsp1407I 3411 TGTACA T^GTACA
5130 TGTACA
BspHI 1225 TCATGA T^CATGA
3231 TCATGA
BspMI 577 GCAGGT ACCTGC(4/8)
4096 GCAGGT
BsrGI 3411 TGTACA T^GTACA
5130 TGTACA
BstXI 1595 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5144 CCACAGCATTGG
BstYI 1797 AGATCC [GA]^GATC[CT]
2804 AGATCT
EcoT14I 2981 CCAAGG C^C[AT][AT]GG
4356 CCTTGG
FbaI 1540 TGATCA T^GATCA
4183 TGATCA
Hin1I 189 GGCGCC G[GA]^CG[CT]C
1717 GGCGCC
HindIII 4058 AAGCTT A^AGCTT
4481 AAGCTT
HpaI 965 GTTAAC GTT^AAC
3872 GTTAAC
KasI 189 GGCGCC G^GCGCC
1717 GGCGCC
MflI 1797 AGATCC [GA]^GATC[CT]
2804 AGATCT
NarI 189 GGCGCC GG^CGCC
1717 GGCGCC
PacI 1426 TTAATTAA TTAAT^TAA
3718 TTAATTAA
PpuMI 3400 GGGACCT [GA]G^G[AT]CC[CT]
5099 GGGTCCC
PshBI 3721 ATTAAT AT^TAAT
4067 ATTAAT
RcaI 1225 TCATGA T^CATGA
3231 TCATGA
SapI 5157 GAAGAGC GCTCTTC(1/4)
328 GCTCTTC
ScaI 3012 AGTACT AGT^ACT
3169 AGTACT
SfoI 189 GGCGCC GGC^GCC
1717 GGCGCC
SmaI 200 CCCGGG CCC^GGG
1769 CCCGGG
SspBI 3411 TGTACA T^GTACA
5130 TGTACA
SspI 382 AATATT AAT^ATT
2778 AATATT
StyI 2981 CCAAGG C^C[AT][AT]GG
4356 CCTTGG
XbaI 2442 TCTAGA T^CTAGA
3145 TCTAGA
XhoII 1797 AGATCC [GA]^GATC[CT]
2804 AGATCT
XmaCI 200 CCCGGG C^CCGGG
1769 CCCGGG
XmaI 200 CCCGGG C^CCGGG
1769 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XmnI