################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccIII AgeI AhdI AscI AspEI AviII AvrII BalI BamHI BbrPI BlnI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI Eam1105I EcoO65I EcoRV FseI FspI KspI MamI MluI MluNI MroI MscI NcoI NheI NotI NruI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I Van91I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 580 GGTACC G^GTACC AclI 945 AACGTT AA^CGTT AflII 592 CTTAAG C^TTAAG AocI 2883 CCTAAGG CC^T[ACGT]AGG Aor51HI 3589 AGCGCT AGC^GCT Asp718I 580 GGTACC G^GTACC BcgI 1751 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 96 GGATAC GTATCC(6/5) BfrI 592 CTTAAG C^TTAAG BglI 4569 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2804 AGATCT A^GATCT BlpI 364 GCTCAGC GC^T[ACGT]AGC Bpu1102I 364 GCTCAGC GC^T[ACGT]AGC BsaAI 4466 TACGTG [CT]AC^GT[GA] BsrBI 145 GAGCGG CCGCTC(-3/-3) BsrFI 4716 GCCGGC [GA]^CCGG[CT] BssSI 1626 CACGAG CACGAG(-5/-1) BstAPI 4578 GCAGGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2883 CCTAAGG CC^T[ACGT]AGG CelII 364 GCTCAGC GC^T[ACGT]AGC Cfr10I 4716 GCCGGC [GA]^CCGG[CT] DraIII 86 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1285 CCGTGG C^C[GA][CT]GG EagI 528 CGGCCG C^GGCCG EclXI 528 CGGCCG C^GGCCG Eco47III 3589 AGCGCT AGC^GCT Eco52I 528 CGGCCG C^GGCCG Eco81I 2883 CCTAAGG CC^T[ACGT]AGG EcoT22I 855 ATGCAT ATGCA^T KpnI 580 GGTACC GGTAC^C NaeI 4716 GCCGGC GCC^GGC NdeI 3969 CATATG CA^TATG NgoMIV 4716 GCCGGC G^CCGGC NsiI 855 ATGCAT ATGCA^T Psp1406I 945 AACGTT AA^CGTT PvuII 4632 CAGCTG CAG^CTG SacI 703 GAGCTC GAGCT^C SphI 2588 GCATGC GCATG^C StuI 1294 AGGCCT AGG^CCT SwaI 1501 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AcyI 189 GGCGCC G[GA]^CG[CT]C 1717 GGCGCC Alw44I 2534 GTGCAC G^TGCAC 4637 GTGCAC ApaI 643 GGGCCC GGGCC^C 1569 GGGCCC ApaLI 2534 GTGCAC G^TGCAC 4637 GTGCAC AseI 3721 ATTAAT AT^TAAT 4067 ATTAAT AsnI 3721 ATTAAT AT^TAAT 4067 ATTAAT BbeI 189 GGCGCC GGCGC^C 1717 GGCGCC BclI 1540 TGATCA T^GATCA 4183 TGATCA BsaHI 189 GGCGCC G[GA]^CG[CT]C 1717 GGCGCC BsaI 1342 GAGACC GGTCTC(1/5) 1643 GAGACC BsiEI 528 CGGCCG CG[GA][CT]^CG 5171 CGGTCG Bsp1407I 3411 TGTACA T^GTACA 5130 TGTACA BspHI 1225 TCATGA T^CATGA 3231 TCATGA BspMI 577 GCAGGT ACCTGC(4/8) 4096 GCAGGT BsrGI 3411 TGTACA T^GTACA 5130 TGTACA BstXI 1595 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5144 CCACAGCATTGG BstYI 1797 AGATCC [GA]^GATC[CT] 2804 AGATCT EcoT14I 2981 CCAAGG C^C[AT][AT]GG 4356 CCTTGG FbaI 1540 TGATCA T^GATCA 4183 TGATCA Hin1I 189 GGCGCC G[GA]^CG[CT]C 1717 GGCGCC HindIII 4058 AAGCTT A^AGCTT 4481 AAGCTT HpaI 965 GTTAAC GTT^AAC 3872 GTTAAC KasI 189 GGCGCC G^GCGCC 1717 GGCGCC MflI 1797 AGATCC [GA]^GATC[CT] 2804 AGATCT NarI 189 GGCGCC GG^CGCC 1717 GGCGCC PacI 1426 TTAATTAA TTAAT^TAA 3718 TTAATTAA PpuMI 3400 GGGACCT [GA]G^G[AT]CC[CT] 5099 GGGTCCC PshBI 3721 ATTAAT AT^TAAT 4067 ATTAAT RcaI 1225 TCATGA T^CATGA 3231 TCATGA SapI 5157 GAAGAGC GCTCTTC(1/4) 328 GCTCTTC ScaI 3012 AGTACT AGT^ACT 3169 AGTACT SfoI 189 GGCGCC GGC^GCC 1717 GGCGCC SmaI 200 CCCGGG CCC^GGG 1769 CCCGGG SspBI 3411 TGTACA T^GTACA 5130 TGTACA SspI 382 AATATT AAT^ATT 2778 AATATT StyI 2981 CCAAGG C^C[AT][AT]GG 4356 CCTTGG XbaI 2442 TCTAGA T^CTAGA 3145 TCTAGA XhoII 1797 AGATCC [GA]^GATC[CT] 2804 AGATCT XmaCI 200 CCCGGG C^CCGGG 1769 CCCGGG XmaI 200 CCCGGG C^CCGGG 1769 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XmnI