##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbeI
BbsI BlnI BpuAI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI CpoI Eam1105I Eco47III Eco81I
HpaI KasI MroI NarI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3215 GGTACC G^GTACC
AccI 645 GTAGAC GT^[AC][GT]AC
AcyI 495 GGCGTC G[GA]^CG[CT]C
AflII 2249 CTTAAG C^TTAAG
Asp718I 3215 GGTACC G^GTACC
AviII 4630 TGCGCA TGC^GCA
BamHI 4240 GGATCC G^GATCC
BcgI 4621 CGAGCCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4995 TGATCA T^GATCA
BfrI 2249 CTTAAG C^TTAAG
BlpI 4524 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4524 GCTAAGC GC^T[ACGT]AGC
BsaHI 495 GGCGTC G[GA]^CG[CT]C
BsmI 626 GAATGC GAATGC(1/-1)
BsrDI 2712 GCAATG GCAATG(2/0)
BssSI 1767 CACGAG CACGAG(-5/-1)
BstAPI 4497 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5299 GGTTACC G^GT[ACGT]ACC
BstPI 5299 GGTTACC G^GT[ACGT]ACC
CelII 4524 GCTAAGC GC^T[ACGT]AGC
DrdI 5102 GACTGTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5299 GGTTACC G^GT[ACGT]ACC
EcoRV 136 GATATC GAT^ATC
FbaI 4995 TGATCA T^GATCA
FseI 4558 GGCCGGCC GGCCGG^CC
FspI 4630 TGCGCA TGC^GCA
Hin1I 495 GGCGTC G[GA]^CG[CT]C
HincII 1667 GTCAAC GT[CT]^[GA]AC
HindII 1667 GTCAAC GT[CT]^[GA]AC
KpnI 3215 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
MfeI 5404 CAATTG C^AATTG
MluI 3886 ACGCGT A^CGCGT
MunI 5404 CAATTG C^AATTG
NdeI 2886 CATATG CA^TATG
NheI 2932 GCTAGC G^CTAGC
SacI 1083 GAGCTC GAGCT^C
SacII 57 CCGCGG CCGC^GG
SfiI 2882 GGCCCATATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4517 CCCGGG CCC^GGG
SspI 596 AATATT AAT^ATT
XmaCI 4517 CCCGGG C^CCGGG
XmaI 4517 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 4762 TGGCCA TGG^CCA
5400 TGGCCA
BglII 2364 AGATCT A^GATCT
5083 AGATCT
BsaBI 447 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5481 GATAAAAATC
BsaWI 1684 ACCGGA [AT]^CCGG[AT]
3499 TCCGGT
Bsp1407I 1511 TGTACA T^GTACA
5392 TGTACA
BsrFI 4555 GCCGGC [GA]^CCGG[CT]
4559 GCCGGC
BsrGI 1511 TGTACA T^GTACA
5392 TGTACA
BssHII 38 GCGCGC G^CGCGC
40 GCGCGC
Cfr10I 4555 GCCGGC [GA]^CCGG[CT]
4559 GCCGGC
DraI 2756 TTTAAA TTT^AAA
5010 TTTAAA
EagI 4557 CGGCCG C^GGCCG
4561 CGGCCG
EclXI 4557 CGGCCG C^GGCCG
4561 CGGCCG
Eco52I 4557 CGGCCG C^GGCCG
4561 CGGCCG
EcoNI 363 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2584 CCTTTACCAGG
EcoRI 1102 GAATTC G^AATTC
4450 GAATTC
HaeII 4332 AGCGCC [GA]GCGC^[CT]
4790 GGCGCT
HgaI 4773 GACGC GACGC(5/10)
496 GCGTC
MamI 447 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5481 GATAAAAATC
MluNI 4762 TGGCCA TGG^CCA
5400 TGGCCA
MscI 4762 TGGCCA TGG^CCA
5400 TGGCCA
NaeI 4555 GCCGGC GCC^GGC
4559 GCCGGC
NgoMIV 4555 GCCGGC G^CCGGC
4559 GCCGGC
PvuII 4222 CAGCTG CAG^CTG
4507 CAGCTG
SspBI 1511 TGTACA T^GTACA
5392 TGTACA
XcmI 570 CCAATAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2878 CCAGGGCCCATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbrPI BbvCI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PmaCI PmlI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII