##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BbeI BcgI BciVI BfrI BglII
BsiWI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HincII HindII HpaI KasI
KpnI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2449 GACGTC GACGT^C
AccIII 2903 TCCGGA T^CCGGA
Alw44I 3990 GTGCAC G^TGCAC
ApaLI 3990 GTGCAC G^TGCAC
AviII 564 TGCGCA TGC^GCA
AvrII 5168 CCTAGG C^CTAGG
BamHI 3289 GGATCC G^GATCC
BbrPI 4758 CACGTG CAC^GTG
BbsI 2131 GTCTTC GAAGAC(2/6)
BlnI 5168 CCTAGG C^CTAGG
BpuAI 2131 GTCTTC GAAGAC(2/6)
BsaAI 4758 CACGTG [CT]AC^GT[GA]
BsaBI 2884 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2903 TCCGGA T^CCGGA
BsiEI 1343 CGGTCG CG[GA][CT]^CG
BsmBI 4806 GAGACG CGTCTC(1/5)
BspDI 1447 ATCGAT AT^CGAT
BspEI 2903 TCCGGA T^CCGGA
BspLU11I 3315 ACATGT A^CATGT
BsrFI 2414 GCCGGT [GA]^CCGG[CT]
BssSI 3227 CTCGTG CACGAG(-5/-1)
BstAPI 2878 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 2414 GCCGGT [GA]^CCGG[CT]
ClaI 1447 ATCGAT AT^CGAT
DrdI 1312 GACTCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 755 GAATTC G^AATTC
EcoT22I 4242 ATGCAT ATGCA^T
FspI 564 TGCGCA TGC^GCA
HindIII 5216 AAGCTT A^AGCTT
KspI 4326 CCGCGG CCGC^GG
MamI 2884 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2903 TCCGGA T^CCGGA
MseI 4393 TTAA T^TAA
NdeI 1114 CATATG CA^TATG
NsiI 4242 ATGCAT ATGCA^T
PmaCI 4758 CACGTG CAC^GTG
PmlI 4758 CACGTG CAC^GTG
PshAI 738 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 5308 GAGCTC GAGCT^C
SacII 4326 CCGCGG CCGC^GG
SfiI 5247 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 716 GATTC G^A[AT]TC
Tru9I 4393 TTAA T^TAA
XbaI 1187 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 745 GTCTAC GT^[AC][GT]AC
1786 GTAGAC
AccII 3739 CGCG CG^CG
4327 CGCG
AcyI 1789 GACGCC G[GA]^CG[CT]C
2449 GACGTC
AflIII 3315 ACATGT A^C[GA][CT]GT
4757 ACACGT
Aor51HI 1531 AGCGCT AGC^GCT
2913 AGCGCT
AspI 301 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
843 GACCTGGTC
BlpI 873 GCTAAGC GC^T[ACGT]AGC
5263 GCTGAGC
Bpu1102I 873 GCTAAGC GC^T[ACGT]AGC
5263 GCTGAGC
BsaHI 1789 GACGCC G[GA]^CG[CT]C
2449 GACGTC
BsaWI 1829 TCCGGT [AT]^CCGG[AT]
2903 TCCGGA
BsmI 523 GCATTC GAATGC(1/-1)
5369 GCATTC
Bsp1407I 1968 TGTACA T^GTACA
5026 TGTACA
BspHI 1769 TCATGA T^CATGA
3233 TCATGA
BsrGI 1968 TGTACA T^GTACA
5026 TGTACA
BstUI 3739 CGCG CG^CG
4327 CGCG
CelII 873 GCTAAGC GC^T[ACGT]AGC
5263 GCTGAGC
DraIII 3850 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4758 CACGTGGTG
Eco47III 1531 AGCGCT AGC^GCT
2913 AGCGCT
Hin1I 1789 GACGCC G[GA]^CG[CT]C
2449 GACGTC
MvnI 3739 CGCG CG^CG
4327 CGCG
PflFI 301 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
843 GACCTGGTC
RcaI 1769 TCATGA T^CATGA
3233 TCATGA
SapI 69 GAAGAGC GCTCTTC(1/4)
1215 GAAGAGC
SexAI 844 ACCTGGT A^CC[AT]GGT
904 ACCAGGT
SspBI 1968 TGTACA T^GTACA
5026 TGTACA
StuI 1144 AGGCCT AGG^CCT
4404 AGGCCT
Tth111I 301 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
843 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BclI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI