##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BamHI BbvCI BcgI BfrI
BglII BlpI Bpu1102I BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KspI MluI MroI MvnI NheI
NotI NruI NsiI NspV PacI
PflMI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SfuI SgrAI SnaBI SpeI StuI
SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 742 GACGTC GACGT^C
Acc65I 4873 GGTACC G^GTACC
AflIII 5187 ACGTGT A^C[GA][CT]GT
AgeI 2257 ACCGGT A^CCGGT
Asp718I 4873 GGTACC G^GTACC
AvrII 282 CCTAGG C^CTAGG
BbeI 2558 GGCGCC GGCGC^C
BbrPI 5186 CACGTG CAC^GTG
BlnI 282 CCTAGG C^CTAGG
BsaWI 2257 ACCGGT [AT]^CCGG[AT]
BsmI 1084 GAATGC GAATGC(1/-1)
Bsp1407I 5143 TGTACA T^GTACA
BspHI 4682 TCATGA T^CATGA
BsrGI 5143 TGTACA T^GTACA
BssSI 1267 CACGAG CACGAG(-5/-1)
BstAPI 657 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2559 GCGC GCG^C
EcoRV 1849 GATATC GAT^ATC
FauI 3892 CCCGC CCCGC(4/6)
HaeII 2558 GGCGCC [GA]GCGC^[CT]
HhaI 2559 GCGC GCG^C
HinP1I 2559 GCGC G^CGC
HindIII 4925 AAGCTT A^AGCTT
KasI 2558 GGCGCC G^GCGCC
KpnI 4873 GGTACC GGTAC^C
NaeI 2689 GCCGGC GCC^GGC
NarI 2558 GGCGCC GG^CGCC
NgoMIV 2689 GCCGGC G^CCGGC
PinAI 2257 ACCGGT A^CCGGT
PmaCI 5186 CACGTG CAC^GTG
PmlI 5186 CACGTG CAC^GTG
RcaI 4682 TCATGA T^CATGA
SfoI 2558 GGCGCC GGC^GCC
SphI 4013 GCATGC GCATG^C
SspBI 5143 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 688 GTCTAC GT^[AC][GT]AC
769 GTCTAC
AciI 3893 CCGC CCGC(-3/-1)
3149 GCGG
Alw44I 1937 GTGCAC G^TGCAC
2450 GTGCAC
ApaLI 1937 GTGCAC G^TGCAC
2450 GTGCAC
Asp700I 3553 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3955 GAAGTCATTC
AspI 934 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1861 GACAAGGTC
BciVI 386 GGATAC GTATCC(6/5)
1587 GGATAC
BfaI 283 CTAG C^TAG
2849 CTAG
BsaAI 2408 TACGTG [CT]AC^GT[GA]
5186 CACGTG
BstXI 4336 CCAGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4403 CCATCAATATGG
DsaI 572 CCATGG C^C[GA][CT]GG
2984 CCATGG
EarI 50 CTCTTC CTCTTC(1/4)
1078 GAAGAG
EcoNI 2221 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4210 CCTAACCAAGG
EcoRI 3800 GAATTC G^AATTC
5197 GAATTC
HgaI 1012 GCGTC GACGC(5/10)
4606 GCGTC
HincII 1390 GTCAAC GT[CT]^[GA]AC
3359 GTCAAC
HindII 1390 GTCAAC GT[CT]^[GA]AC
3359 GTCAAC
Ksp632I 50 CTCTTC CTCTTC(1/4)
1078 GAAGAG
MaeI 283 CTAG C^TAG
2849 CTAG
MfeI 1438 CAATTG C^AATTG
2543 CAATTG
MslI 1158 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4404 CATCAATATG
MunI 1438 CAATTG C^AATTG
2543 CAATTG
NcoI 572 CCATGG C^CATGG
2984 CCATGG
PaeR7I 1006 CTCGAG C^TCGAG
1994 CTCGAG
PflFI 934 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1861 GACAAGGTC
PleI 48 GACTC GAGTC(4/5)
5208 GACTC
SacI 1300 GAGCTC GAGCT^C
2441 GAGCTC
Tth111I 934 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1861 GACAAGGTC
XhoI 1006 CTCGAG C^TCGAG
1994 CTCGAG
XmnI 3553 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3955 GAAGTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AcsI AcyI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BglI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Bsu36I Cac8I Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI EaeI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
Hin1I HinfI HpaII HphI ItaI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SexAI
SfaNI SfcI SfiI SmaI SmlI
Sse8387I SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI