################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AclI AflII AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BfrI BlnI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I CpoI DraIII Eam1105I EcoRV FseI FspI HincII HindII HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SfuI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1716 GACGTC GACGT^C AccI 4526 GTCTAC GT^[AC][GT]AC AgeI 2577 ACCGGT A^CCGGT Alw44I 1860 GTGCAC G^TGCAC ApaLI 1860 GTGCAC G^TGCAC BbeI 5389 GGCGCC GGCGC^C BbrPI 5088 CACGTG CAC^GTG BciVI 2944 GTATCC GTATCC(6/5) BglII 4444 AGATCT A^GATCT BlpI 1816 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1816 GCTCAGC GC^T[ACGT]AGC BsiWI 4207 CGTACG C^GTACG BspDI 5791 ATCGAT AT^CGAT BspHI 240 TCATGA T^CATGA BstEII 3206 GGTCACC G^GT[ACGT]ACC BstPI 3206 GGTCACC G^GT[ACGT]ACC CelII 1816 GCTCAGC GC^T[ACGT]AGC ClaI 5791 ATCGAT AT^CGAT DrdI 2799 GACCACATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4110 CGGCCG C^GGCCG EclXI 4110 CGGCCG C^GGCCG Eco52I 4110 CGGCCG C^GGCCG EcoO65I 3206 GGTCACC G^GT[ACGT]ACC EcoRI 4674 GAATTC G^AATTC KasI 5389 GGCGCC G^GCGCC KspI 5442 CCGCGG CCGC^GG MslI 4489 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 5389 GGCGCC GG^CGCC PinAI 2577 ACCGGT A^CCGGT PmaCI 5088 CACGTG CAC^GTG PmlI 5088 CACGTG CAC^GTG PshAI 3309 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 240 TCATGA T^CATGA SacII 5442 CCGCGG CCGC^GG ScaI 5716 AGTACT AGT^ACT SexAI 343 ACCTGGT A^CC[AT]GGT SfoI 5389 GGCGCC GGC^GCC SgrAI 2576 CACCGGTG C[GA]^CCGG[CT]G SphI 1095 GCATGC GCATG^C SspI 64 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 1342 ACGTGT A^C[GA][CT]GT 4204 ACACGT Aor51HI 1708 AGCGCT AGC^GCT 1820 AGCGCT BclI 230 TGATCA T^GATCA 3868 TGATCA BsaAI 4205 CACGTA [CT]AC^GT[GA] 5088 CACGTG BsaBI 3740 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5784 GATATGTATC BsiEI 1742 CGACCG CG[GA][CT]^CG 4110 CGGCCG BsmBI 4376 CGTCTC CGTCTC(1/5) 5347 CGTCTC BstAPI 2189 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4989 GCAGAGGTTGC Eco47III 1708 AGCGCT AGC^GCT 1820 AGCGCT EcoT22I 125 ATGCAT ATGCA^T 4605 ATGCAT FbaI 230 TGATCA T^GATCA 3868 TGATCA HindIII 1190 AAGCTT A^AGCTT 1509 AAGCTT MamI 3740 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5784 GATATGTATC NaeI 4701 GCCGGC GCC^GGC 5669 GCCGGC NgoMIV 4701 GCCGGC G^CCGGC 5669 GCCGGC NsiI 125 ATGCAT ATGCA^T 4605 ATGCAT SapI 3404 GCTCTTC GCTCTTC(1/4) 5557 GCTCTTC SmaI 1371 CCCGGG CCC^GGG 5689 CCCGGG XmaCI 1371 CCCGGG C^CCGGG 5689 CCCGGG XmaI 1371 CCCGGG C^CCGGG 5689 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII