##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BfrI BlnI BspLU11I
BsrBI BssHII Bst1107I BstBI BstZ17I
CpoI DraIII Eam1105I EcoRV FseI
FspI HincII HindII HpaI KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI SfuI SnaBI SpeI
Sse8387I SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1716 GACGTC GACGT^C
AccI 4526 GTCTAC GT^[AC][GT]AC
AgeI 2577 ACCGGT A^CCGGT
Alw44I 1860 GTGCAC G^TGCAC
ApaLI 1860 GTGCAC G^TGCAC
BbeI 5389 GGCGCC GGCGC^C
BbrPI 5088 CACGTG CAC^GTG
BciVI 2944 GTATCC GTATCC(6/5)
BglII 4444 AGATCT A^GATCT
BlpI 1816 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1816 GCTCAGC GC^T[ACGT]AGC
BsiWI 4207 CGTACG C^GTACG
BspDI 5791 ATCGAT AT^CGAT
BspHI 240 TCATGA T^CATGA
BstEII 3206 GGTCACC G^GT[ACGT]ACC
BstPI 3206 GGTCACC G^GT[ACGT]ACC
CelII 1816 GCTCAGC GC^T[ACGT]AGC
ClaI 5791 ATCGAT AT^CGAT
DrdI 2799 GACCACATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4110 CGGCCG C^GGCCG
EclXI 4110 CGGCCG C^GGCCG
Eco52I 4110 CGGCCG C^GGCCG
EcoO65I 3206 GGTCACC G^GT[ACGT]ACC
EcoRI 4674 GAATTC G^AATTC
KasI 5389 GGCGCC G^GCGCC
KspI 5442 CCGCGG CCGC^GG
MslI 4489 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 5389 GGCGCC GG^CGCC
PinAI 2577 ACCGGT A^CCGGT
PmaCI 5088 CACGTG CAC^GTG
PmlI 5088 CACGTG CAC^GTG
PshAI 3309 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 240 TCATGA T^CATGA
SacII 5442 CCGCGG CCGC^GG
ScaI 5716 AGTACT AGT^ACT
SexAI 343 ACCTGGT A^CC[AT]GGT
SfoI 5389 GGCGCC GGC^GCC
SgrAI 2576 CACCGGTG C[GA]^CCGG[CT]G
SphI 1095 GCATGC GCATG^C
SspI 64 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 1342 ACGTGT A^C[GA][CT]GT
4204 ACACGT
Aor51HI 1708 AGCGCT AGC^GCT
1820 AGCGCT
BclI 230 TGATCA T^GATCA
3868 TGATCA
BsaAI 4205 CACGTA [CT]AC^GT[GA]
5088 CACGTG
BsaBI 3740 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5784 GATATGTATC
BsiEI 1742 CGACCG CG[GA][CT]^CG
4110 CGGCCG
BsmBI 4376 CGTCTC CGTCTC(1/5)
5347 CGTCTC
BstAPI 2189 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4989 GCAGAGGTTGC
Eco47III 1708 AGCGCT AGC^GCT
1820 AGCGCT
EcoT22I 125 ATGCAT ATGCA^T
4605 ATGCAT
FbaI 230 TGATCA T^GATCA
3868 TGATCA
HindIII 1190 AAGCTT A^AGCTT
1509 AAGCTT
MamI 3740 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5784 GATATGTATC
NaeI 4701 GCCGGC GCC^GGC
5669 GCCGGC
NgoMIV 4701 GCCGGC G^CCGGC
5669 GCCGGC
NsiI 125 ATGCAT ATGCA^T
4605 ATGCAT
SapI 3404 GCTCTTC GCTCTTC(1/4)
5557 GCTCTTC
SmaI 1371 CCCGGG CCC^GGG
5689 CCCGGG
XmaCI 1371 CCCGGG C^CCGGG
5689 CCCGGG
XmaI 1371 CCCGGG C^CCGGG
5689 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII