##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AviII BfrI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI Eam1105I Eco47III
EcoRV EcoT22I FseI FspI HpaI
MfeI MluI MroI MunI NcoI
NdeI NotI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 4627 GTGCAC G^TGCAC
ApaLI 4627 GTGCAC G^TGCAC
BamHI 3827 GGATCC G^GATCC
BbrPI 1258 CACGTG CAC^GTG
BbsI 2336 GAAGAC GAAGAC(2/6)
BclI 2975 TGATCA T^GATCA
BglII 2919 AGATCT A^GATCT
BpuAI 2336 GAAGAC GAAGAC(2/6)
BsaBI 5137 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 3113 CATTGC GCAATG(2/0)
CpoI 294 CGGACCG CG^G[AT]CCG
DraIII 2853 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3937 GAATTC G^AATTC
FbaI 2975 TGATCA T^GATCA
HincII 4881 GTCGAC GT[CT]^[GA]AC
HindII 4881 GTCGAC GT[CT]^[GA]AC
HindIII 3733 AAGCTT A^AGCTT
MamI 5137 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1479 GCTAGC G^CTAGC
NruI 628 TCGCGA TCG^CGA
PaeR7I 4564 CTCGAG C^TCGAG
PmaCI 1258 CACGTG CAC^GTG
PmlI 1258 CACGTG CAC^GTG
PstI 697 CTGCAG CTGCA^G
RsrII 294 CGGACCG CG^G[AT]CCG
SalI 4881 GTCGAC G^TCGAC
SfiI 282 GGCCCGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SwaI 1721 ATTTAAAT ATTT^AAAT
XhoI 4564 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2284 GGTACC G^GTACC
4189 GGTACC
AccI 4881 GTCGAC GT^[AC][GT]AC
5460 GTAGAC
Asp718I 2284 GGTACC G^GTACC
4189 GGTACC
AvrII 2878 CCTAGG C^CTAGG
2930 CCTAGG
BcgI 835 GCAGCTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1463 GCAAGGCTCTCG
BciVI 2060 GGATAC GTATCC(6/5)
4006 GGATAC
BglI 283 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2497 GCCAGGAAGGC
BlnI 2878 CCTAGG C^CTAGG
2930 CCTAGG
BsaAI 615 TACGTG [CT]AC^GT[GA]
1258 CACGTG
BspHI 2314 TCATGA T^CATGA
3501 TCATGA
BssHII 67 GCGCGC G^CGCGC
5003 GCGCGC
BstAPI 1492 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4620 GCAACAGGTGC
BstEII 680 GGTCACC G^GT[ACGT]ACC
2111 GGTCACC
BstPI 680 GGTCACC G^GT[ACGT]ACC
2111 GGTCACC
DraI 1722 TTTAAA TTT^AAA
3956 TTTAAA
EarI 1860 CTCTTC CTCTTC(1/4)
5471 GAAGAG
EcoO65I 680 GGTCACC G^GT[ACGT]ACC
2111 GGTCACC
KpnI 2284 GGTACC GGTAC^C
4189 GGTACC
Ksp632I 1860 CTCTTC CTCTTC(1/4)
5471 GAAGAG
MslI 2144 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2825 CACACACATG
NaeI 177 GCCGGC GCC^GGC
1446 GCCGGC
NgoMIV 177 GCCGGC G^CCGGC
1446 GCCGGC
RcaI 2314 TCATGA T^CATGA
3501 TCATGA
SacI 784 GAGCTC GAGCT^C
3144 GAGCTC
SphI 30 GCATGC GCATG^C
3268 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI