##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaI
ApaLI AscI AseI AsnI AvrII
BamHI BcgI BlnI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspEI BspHI
BsrGI BssHII CpoI EagI EclXI
Eco52I EcoRV FseI HindIII KspI
MamI MluI MroI NcoI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2135 GGTACC G^GTACC
AflII 4744 CTTAAG C^TTAAG
AhdI 4997 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4380 AGCGCT AGC^GCT
Asp718I 2135 GGTACC G^GTACC
AspEI 4997 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4034 GTGCTC G[AT]GC[AT]^C
AviII 104 TGCGCA TGC^GCA
BbeI 1676 GGCGCC GGCGC^C
BbrPI 711 CACGTG CAC^GTG
BciVI 63 GGATAC GTATCC(6/5)
BclI 1297 TGATCA T^GATCA
BfrI 4744 CTTAAG C^TTAAG
BglI 2288 GCCTACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 36 CGACCG CG[GA][CT]^CG
BsiHKAI 4034 GTGCTC G[AT]GC[AT]^C
BsmBI 2354 GAGACG CGTCTC(1/5)
BspDI 3971 ATCGAT AT^CGAT
BsrBI 2672 CCGCTC CCGCTC(-3/-3)
BsrDI 4860 GCAATG GCAATG(2/0)
BssSI 504 CACGAG CACGAG(-5/-1)
Bst1107I 494 GTATAC GTA^TAC
BstZ17I 494 GTATAC GTA^TAC
ClaI 3971 ATCGAT AT^CGAT
DraIII 2544 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2027 GACGTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4997 GACCGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4380 AGCGCT AGC^GCT
EcoRI 2015 GAATTC G^AATTC
EcoT22I 645 ATGCAT ATGCA^T
FbaI 1297 TGATCA T^GATCA
FspI 104 TGCGCA TGC^GCA
KasI 1676 GGCGCC G^GCGCC
KpnI 2135 GGTACC GGTAC^C
MfeI 4755 CAATTG C^AATTG
MunI 4755 CAATTG C^AATTG
NaeI 3687 GCCGGC GCC^GGC
NarI 1676 GGCGCC GG^CGCC
NgoMIV 3687 GCCGGC G^CCGGC
NsiI 645 ATGCAT ATGCA^T
PmaCI 711 CACGTG CAC^GTG
PmlI 711 CACGTG CAC^GTG
SfoI 1676 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3951 GACGTC GACGT^C
4735 GACGTC
AccI 494 GTATAC GT^[AC][GT]AC
3907 GTCTAC
AlwNI 85 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1659 CAGCAGCTG
Asp700I 683 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
933 GAAATGCTTC
AvaI 2253 CCCGAG C^[CT]CG[GA]G
4983 CCCGAG
BbvCI 1357 CCTCAGC CCTCAGC(-5/-2)
1647 CCTCAGC
BglII 2055 AGATCT A^GATCT
4302 AGATCT
BlpI 1337 GCTCAGC GC^T[ACGT]AGC
3489 GCTGAGC
Bpu1102I 1337 GCTCAGC GC^T[ACGT]AGC
3489 GCTGAGC
BsaAI 711 CACGTG [CT]AC^GT[GA]
1492 CACGTA
BsmI 643 GAATGC GAATGC(1/-1)
517 GCATTC
BsoBI 2253 CCCGAG C^[CT]CG[GA]G
4983 CCCGAG
BsrFI 134 ACCGGC [GA]^CCGG[CT]
3687 GCCGGC
BstAPI 1611 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2690 GCATACGGTGC
BstBI 3300 TTCGAA TT^CGAA
4076 TTCGAA
BstXI 2040 CCAAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2727 CCATTTATTTGG
CelII 1337 GCTCAGC GC^T[ACGT]AGC
3489 GCTGAGC
Cfr10I 134 ACCGGC [GA]^CCGG[CT]
3687 GCCGGC
HgaI 3882 GACGC GACGC(5/10)
3922 GACGC
HpaI 2122 GTTAAC GTT^AAC
2744 GTTAAC
MslI 3339 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4951 CACAGCCGTG
NdeI 264 CATATG CA^TATG
1906 CATATG
NspV 3300 TTCGAA TT^CGAA
4076 TTCGAA
PshAI 3882 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4339 GACCGGGGTC
PstI 3447 CTGCAG CTGCA^G
5036 CTGCAG
SapI 4852 GAAGAGC GCTCTTC(1/4)
2622 GCTCTTC
SexAI 3096 ACCTGGT A^CC[AT]GGT
4509 ACCTGGT
SfuI 3300 TTCGAA TT^CGAA
4076 TTCGAA
XbaI 298 TCTAGA T^CTAGA
3650 TCTAGA
XmnI 683 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
933 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
AspI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII