##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI AscI Asp718I AspEI
AspI BcgI BfrI BsaBI BsaI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstUI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV FseI
HgaI HincII HindII HpaI KpnI
KspI MamI MluI MroI MvnI
NheI NotI NruI NspV PacI
PflFI PinAI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 4363 GTCTAC GT^[AC][GT]AC
AclI 1289 AACGTT AA^CGTT
Alw44I 1176 GTGCAC G^TGCAC
Aor51HI 3874 AGCGCT AGC^GCT
ApaI 2164 GGGCCC GGGCC^C
ApaLI 1176 GTGCAC G^TGCAC
AviII 3056 TGCGCA TGC^GCA
AvrII 1668 CCTAGG C^CTAGG
BalI 3067 TGGCCA TGG^CCA
BamHI 2007 GGATCC G^GATCC
BbrPI 839 CACGTG CAC^GTG
BclI 3526 TGATCA T^GATCA
BglII 600 AGATCT A^GATCT
BlnI 1668 CCTAGG C^CTAGG
BlpI 2458 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2458 GCTTAGC GC^T[ACGT]AGC
BsaAI 839 CACGTG [CT]AC^GT[GA]
BsaWI 4423 ACCGGA [AT]^CCGG[AT]
BsmBI 3926 GAGACG CGTCTC(1/5)
Bsp1407I 845 TGTACA T^GTACA
BspLU11I 4928 ACATGT A^CATGT
BsrFI 3758 GCCGGC [GA]^CCGG[CT]
BsrGI 845 TGTACA T^GTACA
BssSI 115 CACGAG CACGAG(-5/-1)
BstEII 2939 GGTAACC G^GT[ACGT]ACC
BstPI 2939 GGTAACC G^GT[ACGT]ACC
CelII 2458 GCTTAGC GC^T[ACGT]AGC
Cfr10I 3758 GCCGGC [GA]^CCGG[CT]
DraIII 3593 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3067 TGGCCA [CT]^GGCC[GA]
Eco47III 3874 AGCGCT AGC^GCT
EcoO65I 2939 GGTAACC G^GT[ACGT]ACC
EcoRI 2513 GAATTC G^AATTC
FbaI 3526 TGATCA T^GATCA
FspI 3056 TGCGCA TGC^GCA
MluNI 3067 TGGCCA TGG^CCA
MscI 3067 TGGCCA TGG^CCA
NaeI 3758 GCCGGC GCC^GGC
NdeI 1136 CATATG CA^TATG
NgoMIV 3758 GCCGGC G^CCGGC
PaeR7I 1792 CTCGAG C^TCGAG
PmaCI 839 CACGTG CAC^GTG
PmeI 3266 GTTTAAAC GTTT^AAAC
PmlI 839 CACGTG CAC^GTG
Psp1406I 1289 AACGTT AA^CGTT
SacI 1557 GAGCTC GAGCT^C
ScaI 4734 AGTACT AGT^ACT
SmaI 4267 CCCGGG CCC^GGG
SpeI 3400 ACTAGT A^CTAGT
SspBI 845 TGTACA T^GTACA
StuI 3693 AGGCCT AGG^CCT
XhoI 1792 CTCGAG C^TCGAG
XmaCI 4267 CCCGGG C^CCGGG
XmaI 4267 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 840 ACGTGT A^C[GA][CT]GT
4928 ACATGT
AseI 6 ATTAAT AT^TAAT
4004 ATTAAT
AsnI 6 ATTAAT AT^TAAT
4004 ATTAAT
AvaI 1792 CTCGAG C^[CT]CG[GA]G
4267 CCCGGG
BbsI 937 GAAGAC GAAGAC(2/6)
1084 GAAGAC
BciVI 860 GGATAC GTATCC(6/5)
2736 GTATCC
BglI 2157 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4445 GCCCCTATGGC
BpuAI 937 GAAGAC GAAGAC(2/6)
1084 GAAGAC
BsoBI 1792 CTCGAG C^[CT]CG[GA]G
4267 CCCGGG
BstAPI 2217 GCATCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4033 GCATGTAATGC
DrdI 1640 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3612 GACTCATTTGTC
EcoNI 94 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
776 CCTTTATAAGG
FauI 1027 CCCGC CCCGC(4/6)
3788 GCGGG
MfeI 806 CAATTG C^AATTG
2974 CAATTG
MunI 806 CAATTG C^AATTG
2974 CAATTG
PpuMI 771 AGGACCC [GA]G^G[AT]CC[CT]
1477 AGGTCCC
PshAI 2267 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2816 GACTATAGTC
PshBI 6 ATTAAT AT^TAAT
4004 ATTAAT
PstI 1171 CTGCAG CTGCA^G
1485 CTGCAG
PvuII 2169 CAGCTG CAG^CTG
2207 CAGCTG
SphI 1813 GCATGC GCATG^C
4517 GCATGC
TaqI 543 TCGA T^CGA
1793 TCGA
TthHB8I 543 TCGA T^CGA
1793 TCGA
XcmI 616 CCAAACTGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3011 CCATGCAGATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI