##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AviII
BamHI BanI BbeI BbrPI BciVI
BcnI BglII BpmI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BstEII
BstPI BstUI BstYI CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MflI MluI MroI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI PvuII RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SwaI Van91I
XcmI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AclI 3349 AACGTT AA^CGTT
AflII 395 CTTAAG C^TTAAG
AflIII 3034 ACATGT A^C[GA][CT]GT
AlwNI 2587 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4731 CCTCAGG CC^T[ACGT]AGG
AvrII 3160 CCTAGG C^CTAGG
BcgI 3371 CGAATGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4912 TGATCA T^GATCA
BfrI 395 CTTAAG C^TTAAG
BglI 4584 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3160 CCTAGG C^CTAGG
BlpI 461 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 461 GCTAAGC GC^T[ACGT]AGC
BsaI 4049 GAGACC GGTCTC(1/5)
BsaWI 3867 ACCGGA [AT]^CCGG[AT]
BsgI 3361 CTGCAC GTGCAG(16/14)
BsmBI 4143 GAGACG CGTCTC(1/5)
BsmI 1571 GAATGC GAATGC(1/-1)
BspHI 2568 TCATGA T^CATGA
BspLU11I 3034 ACATGT A^CATGT
BspMI 3698 GCAGGT ACCTGC(4/8)
BsrDI 3834 CATTGC GCAATG(2/0)
BssSI 4573 CTCGTG CACGAG(-5/-1)
BstAPI 3411 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 240 TTCGAA TT^CGAA
Bsu36I 4731 CCTCAGG CC^T[ACGT]AGG
CelII 461 GCTAAGC GC^T[ACGT]AGC
DrdI 3986 GACATTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4731 CCTCAGG CC^T[ACGT]AGG
EcoRV 641 GATATC GAT^ATC
FbaI 4912 TGATCA T^GATCA
HapII 3868 CCGG C^CGG
HgaI 335 GACGC GACGC(5/10)
HpaI 1379 GTTAAC GTT^AAC
HpaII 3868 CCGG C^CGG
MfeI 354 CAATTG C^AATTG
MspI 3868 CCGG C^CGG
MunI 354 CAATTG C^AATTG
NheI 3381 GCTAGC G^CTAGC
NspV 240 TTCGAA TT^CGAA
PaeR7I 2822 CTCGAG C^TCGAG
PpuMI 2755 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 3349 AACGTT AA^CGTT
RcaI 2568 TCATGA T^CATGA
SacI 1753 GAGCTC GAGCT^C
SapI 3871 GAAGAGC GCTCTTC(1/4)
ScaI 1109 AGTACT AGT^ACT
SexAI 3732 ACCAGGT A^CC[AT]GGT
SfuI 240 TTCGAA TT^CGAA
SphI 4610 GCATGC GCATG^C
StuI 5007 AGGCCT AGG^CCT
XhoI 2822 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 3385 GCGG CCGC(-3/-1)
4602 GCGG
Asp700I 276 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1295 GAAGGATTTC
AspI 596 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4453 GACCTCGTC
AvaI 2822 CTCGAG C^[CT]CG[GA]G
3424 CTCGGG
BalI 207 TGGCCA TGG^CCA
4487 TGGCCA
BbvCI 221 GCTGAGG CCTCAGC(-5/-2)
3652 GCTGAGG
BsoBI 2822 CTCGAG C^[CT]CG[GA]G
3424 CTCGGG
Bst1107I 2462 GTATAC GTA^TAC
3496 GTATAC
BstZ17I 2462 GTATAC GTA^TAC
3496 GTATAC
EcoNI 113 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4316 CCTGAAAAAGG
EcoRI 4098 GAATTC G^AATTC
4792 GAATTC
MluNI 207 TGGCCA TGG^CCA
4487 TGGCCA
MscI 207 TGGCCA TGG^CCA
4487 TGGCCA
PflFI 596 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4453 GACCTCGTC
SpeI 2645 ACTAGT A^CTAGT
3965 ACTAGT
Tth111I 596 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4453 GACCTCGTC
XbaI 1589 TCTAGA T^CTAGA
2445 TCTAGA
XmnI 276 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1295 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I