##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I AocI ApaI ApaLI
AscI AspI AviII BbeI BbrPI
BcgI BciVI BglI BglII BsaAI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
FauI FseI FspI HgaI Hin1I
KasI KspI MaeII MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3563 GGTACC G^GTACC
AflII 246 CTTAAG C^TTAAG
AhdI 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 122 AGCGCT AGC^GCT
Asp700I 4002 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3563 GGTACC G^GTACC
AspEI 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1996 CCCGAG C^[CT]CG[GA]G
BalI 1350 TGGCCA TGG^CCA
BamHI 1567 GGATCC G^GATCC
BanI 3563 GGTACC G^G[CT][GA]CC
BanII 1902 GGGCTC G[GA]GC[CT]^C
BclI 4240 TGATCA T^GATCA
BfrI 246 CTTAAG C^TTAAG
BlpI 2752 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2752 GCTCAGC GC^T[ACGT]AGC
BsaBI 4309 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 177 TCCGGT [AT]^CCGG[AT]
BsmI 1342 GAATGC GAATGC(1/-1)
BsoBI 1996 CCCGAG C^[CT]CG[GA]G
CelII 2752 GCTCAGC GC^T[ACGT]AGC
CfoI 123 GCGC GCG^C
DraIII 2230 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3567 CCATGG C^C[GA][CT]GG
EaeI 1350 TGGCCA [CT]^GGCC[GA]
Eam1105I 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 122 AGCGCT AGC^GCT
EcoRI 2255 GAATTC G^AATTC
FbaI 4240 TGATCA T^GATCA
HaeII 122 AGCGCT [GA]GCGC^[CT]
HhaI 123 GCGC GCG^C
HinP1I 123 GCGC G^CGC
HincII 4530 GTTAAC GT[CT]^[GA]AC
HindII 4530 GTTAAC GT[CT]^[GA]AC
HpaI 4530 GTTAAC GTT^AAC
KpnI 3563 GGTACC GGTAC^C
MamI 4309 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1350 TGGCCA TGG^CCA
MscI 1350 TGGCCA TGG^CCA
NcoI 3567 CCATGG C^CATGG
NheI 8 GCTAGC G^CTAGC
SwaI 5133 ATTTAAAT ATTT^AAAT
XmnI 4002 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 116 GCGG CCGC(-3/-1)
2328 GCGG
AlwNI 965 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1514 CAGGCACTG
AspHI 1655 GTGCTC G[AT]GC[AT]^C
2750 GTGCTC
AvrII 1556 CCTAGG C^CTAGG
1579 CCTAGG
BcnI 48 CCCGG CC^[GC]GG
108 CCGGG
BlnI 1556 CCTAGG C^CTAGG
1579 CCTAGG
BsaI 136 GAGACC GGTCTC(1/5)
1333 GAGACC
BseRI 1916 CTCCTC GAGGAG(10/8)
1330 GAGGAG
BsiHKAI 1655 GTGCTC G[AT]GC[AT]^C
2750 GTGCTC
Bst1107I 417 GTATAC GTA^TAC
2565 GTATAC
BstXI 2047 CCATATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2387 CCAAAGTGTTGG
BstZ17I 417 GTATAC GTA^TAC
2565 GTATAC
DraII 3671 AGGTCCT [GA]G^G[ACGT]CC[CT]
3872 GGGACCT
EcoO109I 3671 AGGTCCT [GA]G^G[ACGT]CC[CT]
3872 GGGACCT
EcoRV 1798 GATATC GAT^ATC
5297 GATATC
EcoT22I 191 ATGCAT ATGCA^T
861 ATGCAT
HindIII 529 AAGCTT A^AGCTT
3040 AAGCTT
NciI 48 CCCGG CC^[GC]GG
108 CCGGG
NsiI 191 ATGCAT ATGCA^T
861 ATGCAT
PleI 967 GACTC GAGTC(4/5)
3922 GAGTC
PpuMI 3671 AGGTCCT [GA]G^G[AT]CC[CT]
3872 GGGACCT
ScaI 1035 AGTACT AGT^ACT
4831 AGTACT
StuI 787 AGGCCT AGG^CCT
2061 AGGCCT
TaqI 128 TCGA T^CGA
2467 TCGA
TthHB8I 128 TCGA T^CGA
2467 TCGA
XbaI 3916 TCTAGA T^CTAGA
3925 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII