################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI Alw44I AocI ApaI ApaLI AscI AspI AviII BbeI BbrPI BcgI BciVI BglI BglII BsaAI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO65I FauI FseI FspI HgaI Hin1I KasI KspI MaeII MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 3563 GGTACC G^GTACC AflII 246 CTTAAG C^TTAAG AhdI 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 122 AGCGCT AGC^GCT Asp700I 4002 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3563 GGTACC G^GTACC AspEI 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1996 CCCGAG C^[CT]CG[GA]G BalI 1350 TGGCCA TGG^CCA BamHI 1567 GGATCC G^GATCC BanI 3563 GGTACC G^G[CT][GA]CC BanII 1902 GGGCTC G[GA]GC[CT]^C BclI 4240 TGATCA T^GATCA BfrI 246 CTTAAG C^TTAAG BlpI 2752 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2752 GCTCAGC GC^T[ACGT]AGC BsaBI 4309 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 177 TCCGGT [AT]^CCGG[AT] BsmI 1342 GAATGC GAATGC(1/-1) BsoBI 1996 CCCGAG C^[CT]CG[GA]G CelII 2752 GCTCAGC GC^T[ACGT]AGC CfoI 123 GCGC GCG^C DraIII 2230 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 3567 CCATGG C^C[GA][CT]GG EaeI 1350 TGGCCA [CT]^GGCC[GA] Eam1105I 3665 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 122 AGCGCT AGC^GCT EcoRI 2255 GAATTC G^AATTC FbaI 4240 TGATCA T^GATCA HaeII 122 AGCGCT [GA]GCGC^[CT] HhaI 123 GCGC GCG^C HinP1I 123 GCGC G^CGC HincII 4530 GTTAAC GT[CT]^[GA]AC HindII 4530 GTTAAC GT[CT]^[GA]AC HpaI 4530 GTTAAC GTT^AAC KpnI 3563 GGTACC GGTAC^C MamI 4309 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1350 TGGCCA TGG^CCA MscI 1350 TGGCCA TGG^CCA NcoI 3567 CCATGG C^CATGG NheI 8 GCTAGC G^CTAGC SwaI 5133 ATTTAAAT ATTT^AAAT XmnI 4002 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 116 GCGG CCGC(-3/-1) 2328 GCGG AlwNI 965 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 1514 CAGGCACTG AspHI 1655 GTGCTC G[AT]GC[AT]^C 2750 GTGCTC AvrII 1556 CCTAGG C^CTAGG 1579 CCTAGG BcnI 48 CCCGG CC^[GC]GG 108 CCGGG BlnI 1556 CCTAGG C^CTAGG 1579 CCTAGG BsaI 136 GAGACC GGTCTC(1/5) 1333 GAGACC BseRI 1916 CTCCTC GAGGAG(10/8) 1330 GAGGAG BsiHKAI 1655 GTGCTC G[AT]GC[AT]^C 2750 GTGCTC Bst1107I 417 GTATAC GTA^TAC 2565 GTATAC BstXI 2047 CCATATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2387 CCAAAGTGTTGG BstZ17I 417 GTATAC GTA^TAC 2565 GTATAC DraII 3671 AGGTCCT [GA]G^G[ACGT]CC[CT] 3872 GGGACCT EcoO109I 3671 AGGTCCT [GA]G^G[ACGT]CC[CT] 3872 GGGACCT EcoRV 1798 GATATC GAT^ATC 5297 GATATC EcoT22I 191 ATGCAT ATGCA^T 861 ATGCAT HindIII 529 AAGCTT A^AGCTT 3040 AAGCTT NciI 48 CCCGG CC^[GC]GG 108 CCGGG NsiI 191 ATGCAT ATGCA^T 861 ATGCAT PleI 967 GACTC GAGTC(4/5) 3922 GAGTC PpuMI 3671 AGGTCCT [GA]G^G[AT]CC[CT] 3872 GGGACCT ScaI 1035 AGTACT AGT^ACT 4831 AGTACT StuI 787 AGGCCT AGG^CCT 2061 AGGCCT TaqI 128 TCGA T^CGA 2467 TCGA TthHB8I 128 TCGA T^CGA 2467 TCGA XbaI 3916 TCTAGA T^CTAGA 3925 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AvaII BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII