##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AhdI AscI Asp700I AspEI AvaI
AviII BalI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI DrdI EaeI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FbaI
FseI FspI KasI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI SacI SacII SalI SbfI
ScaI SexAI SfoI SfuI SmaI
SnaBI Sse8387I SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4055 GGTACC G^GTACC
AclI 5264 AACGTT AA^CGTT
AcyI 184 GACGCC G[GA]^CG[CT]C
Alw44I 3609 GTGCAC G^TGCAC
Aor51HI 3404 AGCGCT AGC^GCT
ApaLI 3609 GTGCAC G^TGCAC
AseI 3371 ATTAAT AT^TAAT
AsnI 3371 ATTAAT AT^TAAT
Asp718I 4055 GGTACC G^GTACC
AspI 803 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5444 CCTAGG C^CTAGG
BlnI 5444 CCTAGG C^CTAGG
BsaBI 3465 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 184 GACGCC G[GA]^CG[CT]C
BsaWI 2607 ACCGGA [AT]^CCGG[AT]
BspLU11I 1016 ACATGT A^CATGT
BspMI 3578 ACCTGC ACCTGC(4/8)
CpoI 97 CGGTCCG CG^G[AT]CCG
DraIII 3484 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 359 CCATGG C^C[GA][CT]GG
Eco47III 3404 AGCGCT AGC^GCT
EcoT22I 1631 ATGCAT ATGCA^T
Hin1I 184 GACGCC G[GA]^CG[CT]C
HpaI 4784 GTTAAC GTT^AAC
KpnI 4055 GGTACC GGTAC^C
MamI 3465 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 359 CCATGG C^CATGG
NheI 213 GCTAGC G^CTAGC
NsiI 1631 ATGCAT ATGCA^T
PflFI 803 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3371 ATTAAT AT^TAAT
Psp1406I 5264 AACGTT AA^CGTT
RsrII 97 CGGTCCG CG^G[AT]CCG
SfiI 3880 GGCCCTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 28 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 4657 ACTAGT A^CTAGT
SphI 2099 GCATGC GCATG^C
SspI 3656 AATATT AAT^ATT
StuI 493 AGGCCT AGG^CCT
Tth111I 803 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1016 ACATGT A^C[GA][CT]GT
1032 ACGTGT
ApaI 932 GGGCCC GGGCC^C
1074 GGGCCC
BamHI 152 GGATCC G^GATCC
4997 GGATCC
BsaI 1741 GAGACC GGTCTC(1/5)
4761 GGTCTC
BsmBI 4336 CGTCTC CGTCTC(1/5)
4846 CGTCTC
BsmI 855 GAATGC GAATGC(1/-1)
2596 GAATGC
BspHI 1748 TCATGA T^CATGA
4311 TCATGA
BsrFI 29 GCCGGT [GA]^CCGG[CT]
1619 ACCGGC
BstEII 4670 GGTGACC G^GT[ACGT]ACC
5280 GGTTACC
BstPI 4670 GGTGACC G^GT[ACGT]ACC
5280 GGTTACC
BstXI 4223 CCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5210 CCACATTTTTGG
Cfr10I 29 GCCGGT [GA]^CCGG[CT]
1619 ACCGGC
EcoO65I 4670 GGTGACC G^GT[ACGT]ACC
5280 GGTTACC
HaeII 1261 AGCGCC [GA]GCGC^[CT]
3404 AGCGCT
HgaI 184 GACGC GACGC(5/10)
1 GCGTC
HincII 1894 GTCAAC GT[CT]^[GA]AC
4784 GTTAAC
HindII 1894 GTCAAC GT[CT]^[GA]AC
4784 GTTAAC
HindIII 913 AAGCTT A^AGCTT
4235 AAGCTT
PstI 1292 CTGCAG CTGCA^G
3263 CTGCAG
RcaI 1748 TCATGA T^CATGA
4311 TCATGA
SmlI 412 CTTGAG C^T[CT][GA]AG
5109 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII