##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BciVI BfrI BglI
BlnI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BsrFI BssHII BstAPI BstBI
BstUI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FseI FspI HaeII KasI
KpnI KspI MluI MroI MspA1I
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Alw44I 1641 GTGCAC G^TGCAC
AocI 456 CCTCAGG CC^T[ACGT]AGG
ApaLI 1641 GTGCAC G^TGCAC
BalI 3558 TGGCCA TGG^CCA
BamHI 220 GGATCC G^GATCC
BanI 3814 GGTGCC G^G[CT][GA]CC
BbsI 3834 GAAGAC GAAGAC(2/6)
BcgI 934 CGAGAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5721 TGATCA T^GATCA
BcnI 3213 CCCGG CC^[GC]GG
BglII 3586 AGATCT A^GATCT
BpuAI 3834 GAAGAC GAAGAC(2/6)
BsaAI 4835 TACGTA [CT]AC^GT[GA]
BsgI 2951 GTGCAG GTGCAG(16/14)
BsrBI 119 GAGCGG CCGCTC(-3/-3)
BssSI 975 CACGAG CACGAG(-5/-1)
BstEII 2568 GGTAACC G^GT[ACGT]ACC
BstPI 2568 GGTAACC G^GT[ACGT]ACC
BstXI 4074 CCAAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 456 CCTCAGG CC^T[ACGT]AGG
CfoI 904 GCGC GCG^C
DraII 4135 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 3309 GACCAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4966 CCATGG C^C[GA][CT]GG
EaeI 3558 TGGCCA [CT]^GGCC[GA]
Eco81I 456 CCTCAGG CC^T[ACGT]AGG
EcoNI 32 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4135 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2568 GGTAACC G^GT[ACGT]ACC
EcoT14I 4966 CCATGG C^C[AT][AT]GG
FauI 588 CCCGC CCCGC(4/6)
FbaI 5721 TGATCA T^GATCA
HapII 3214 CCGG C^CGG
HhaI 904 GCGC GCG^C
HinP1I 904 GCGC G^CGC
HpaI 407 GTTAAC GTT^AAC
HpaII 3214 CCGG C^CGG
MfeI 4641 CAATTG C^AATTG
MluNI 3558 TGGCCA TGG^CCA
MscI 3558 TGGCCA TGG^CCA
MspI 3214 CCGG C^CGG
MunI 4641 CAATTG C^AATTG
NciI 3213 CCCGG CC^[GC]GG
NcoI 4966 CCATGG C^CATGG
PstI 2279 CTGCAG CTGCA^G
SapI 116 GAAGAGC GCTCTTC(1/4)
SnaBI 4835 TACGTA TAC^GTA
StyI 4966 CCATGG C^C[AT][AT]GG
XcmI 3719 CCATGCTAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AclI 5491 AACGTT AA^CGTT
5844 AACGTT
AcyI 738 GGCGTC G[GA]^CG[CT]C
780 GACGCC
AvaII 623 GGACC G^G[AT]CC
3308 GGACC
BbvCI 2309 CCTCAGC CCTCAGC(-5/-2)
71 GCTGAGG
BlpI 5546 GCTAAGC GC^T[ACGT]AGC
5714 GCTGAGC
Bpu1102I 5546 GCTAAGC GC^T[ACGT]AGC
5714 GCTGAGC
BsaHI 738 GGCGTC G[GA]^CG[CT]C
780 GACGCC
BsaJI 3212 CCCCGG C^C[ACGT][ACGT]GG
4966 CCATGG
BseRI 1562 CTCCTC GAGGAG(10/8)
4061 CTCCTC
BsmI 1248 GCATTC GAATGC(1/-1)
5741 GCATTC
Bst1107I 4512 GTATAC GTA^TAC
5590 GTATAC
BstZ17I 4512 GTATAC GTA^TAC
5590 GTATAC
CelII 5546 GCTAAGC GC^T[ACGT]AGC
5714 GCTGAGC
EcoT22I 671 ATGCAT ATGCA^T
5033 ATGCAT
HgaI 780 GACGC GACGC(5/10)
739 GCGTC
Hin1I 738 GGCGTC G[GA]^CG[CT]C
780 GACGCC
HindIII 3915 AAGCTT A^AGCTT
5425 AAGCTT
HphI 2149 GGTGA GGTGA(8/7)
4691 TCACC
NsiI 671 ATGCAT ATGCA^T
5033 ATGCAT
PacI 1502 TTAATTAA TTAAT^TAA
5119 TTAATTAA
Psp1406I 5491 AACGTT AA^CGTT
5844 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
BbvI BfaI BmyI BpmI BsaBI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoRV Fnu4HI
FokI HaeIII HincII HindII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII