##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII BbeI BbrPI BbvCI
BcgI BciVI BglI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BspEI
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I Cfr10I CpoI DraIII EagI
EclXI Eco47III Eco52I FseI FspI
HaeII KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 576 GACGTC GACGT^C
AflII 4700 CTTAAG C^TTAAG
AhdI 672 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4863 ATTAAT AT^TAAT
AsnI 4863 ATTAAT AT^TAAT
AspEI 672 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2979 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3341 CCTAGG C^CTAGG
BalI 1274 TGGCCA TGG^CCA
BamHI 3702 GGATCC G^GATCC
BfrI 4700 CTTAAG C^TTAAG
BlnI 3341 CCTAGG C^CTAGG
BlpI 1103 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1103 GCTTAGC GC^T[ACGT]AGC
BsgI 1486 GTGCAG GTGCAG(16/14)
BsmBI 3259 CGTCTC CGTCTC(1/5)
BsmI 442 GCATTC GAATGC(1/-1)
Bsp1407I 3990 TGTACA T^GTACA
BspDI 310 ATCGAT AT^CGAT
BsrBI 1328 CCGCTC CCGCTC(-3/-3)
BsrGI 3990 TGTACA T^GTACA
BssSI 3299 CACGAG CACGAG(-5/-1)
BstAPI 1905 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1312 GGTCACC G^GT[ACGT]ACC
BstPI 1312 GGTCACC G^GT[ACGT]ACC
CelII 1103 GCTTAGC GC^T[ACGT]AGC
CfoI 2022 GCGC GCG^C
ClaI 310 ATCGAT AT^CGAT
DrdI 1168 GACACCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 672 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1312 GGTCACC G^GT[ACGT]ACC
EcoRI 810 GAATTC G^AATTC
FauI 3376 CCCGC CCCGC(4/6)
HgaI 2000 GACGC GACGC(5/10)
HhaI 2022 GCGC GCG^C
HinP1I 2022 GCGC G^CGC
HpaI 1662 GTTAAC GTT^AAC
MfeI 1024 CAATTG C^AATTG
MluNI 1274 TGGCCA TGG^CCA
MscI 1274 TGGCCA TGG^CCA
MunI 1024 CAATTG C^AATTG
NdeI 5170 CATATG CA^TATG
PaeR7I 589 CTCGAG C^TCGAG
PflFI 2979 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4863 ATTAAT AT^TAAT
PstI 1912 CTGCAG CTGCA^G
ScaI 1802 AGTACT AGT^ACT
SspBI 3990 TGTACA T^GTACA
SspI 4892 AATATT AAT^ATT
StuI 3303 AGGCCT AGG^CCT
Tth111I 2979 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 589 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 1328 CCGC CCGC(-3/-1)
3377 CCGC
AcyI 576 GACGTC G[GA]^CG[CT]C
2000 GACGCC
AlwNI 1432 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2932 CAGTTTCTG
AocI 1888 CCTCAGG CC^T[ACGT]AGG
3951 CCTAAGG
AvaI 376 CCCGAG C^[CT]CG[GA]G
589 CTCGAG
BanI 2549 GGTGCC G^G[CT][GA]CC
2826 GGTGCC
BcnI 2836 CCCGG CC^[GC]GG
3415 CCCGG
BsaHI 576 GACGTC G[GA]^CG[CT]C
2000 GACGCC
BsaI 1452 GAGACC GGTCTC(1/5)
2731 GAGACC
BsoBI 376 CCCGAG C^[CT]CG[GA]G
589 CTCGAG
BspHI 2264 TCATGA T^CATGA
2701 TCATGA
BstXI 1277 CCAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3216 CCAGTTTCATGG
Bsu36I 1888 CCTCAGG CC^T[ACGT]AGG
3951 CCTAAGG
DsaI 1271 CCATGG C^C[GA][CT]GG
2140 CCATGG
EaeI 1274 TGGCCA [CT]^GGCC[GA]
3276 CGGCCA
Eco81I 1888 CCTCAGG CC^T[ACGT]AGG
3951 CCTAAGG
EcoRV 75 GATATC GAT^ATC
2681 GATATC
EcoT22I 625 ATGCAT ATGCA^T
3836 ATGCAT
Hin1I 576 GACGTC G[GA]^CG[CT]C
2000 GACGCC
MspA1I 717 CAGCTG C[AC]G^C[GT]G
3154 CAGCTG
NciI 2836 CCCGG CC^[GC]GG
3415 CCCGG
NcoI 1271 CCATGG C^CATGG
2140 CCATGG
NsiI 625 ATGCAT ATGCA^T
3836 ATGCAT
PflMI 1302 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1696 CCAAAGAATGG
PvuII 717 CAGCTG CAG^CTG
3154 CAGCTG
RcaI 2264 TCATGA T^CATGA
2701 TCATGA
SacI 1670 GAGCTC GAGCT^C
4044 GAGCTC
SfcI 1756 CTGTAG C^T[GA][CT]AG
1912 CTGCAG
Van91I 1302 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1696 CCAAAGAATGG
XbaI 824 TCTAGA T^CTAGA
3192 TCTAGA
XcmI 400 CCACTCTCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2005 CCACAAGTCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI