##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII Aor51HI AscI
AseI AsnI Asp700I Asp718I BclI
BfrI BglII BsaBI Bsp1407I BspDI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoRV FbaI FseI HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshBI Psp1406I RsrII
SalI SapI SbfI SexAI SfuI
SnaBI SpeI Sse8387I SspBI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 319 GTAGAC GT^[AC][GT]AC
AccIII 5056 TCCGGA T^CCGGA
AgeI 5393 ACCGGT A^CCGGT
AhdI 667 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 667 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 653 TGCGCA TGC^GCA
AvrII 4789 CCTAGG C^CTAGG
BamHI 2324 GGATCC G^GATCC
BbrPI 5282 CACGTG CAC^GTG
BciVI 5351 GTATCC GTATCC(6/5)
BlnI 4789 CCTAGG C^CTAGG
BseAI 5056 TCCGGA T^CCGGA
BsiWI 3548 CGTACG C^GTACG
BspEI 5056 TCCGGA T^CCGGA
BspLU11I 1435 ACATGT A^CATGT
BssSI 4461 CTCGTG CACGAG(-5/-1)
DraI 2517 TTTAAA TTT^AAA
DrdI 1344 GACAGAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 667 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1939 CTCTTC CTCTTC(1/4)
EcoRI 4357 GAATTC G^AATTC
FspI 653 TGCGCA TGC^GCA
HincII 5035 GTCAAC GT[CT]^[GA]AC
HindII 5035 GTCAAC GT[CT]^[GA]AC
HindIII 2344 AAGCTT A^AGCTT
Ksp632I 1939 CTCTTC CTCTTC(1/4)
KspI 796 CCGCGG CCGC^GG
MroI 5056 TCCGGA T^CCGGA
NaeI 809 GCCGGC GCC^GGC
NgoMIV 809 GCCGGC G^CCGGC
PflFI 3392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5393 ACCGGT A^CCGGT
PmaCI 5282 CACGTG CAC^GTG
PmlI 5282 CACGTG CAC^GTG
PshAI 5028 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 36 CGATCG CGAT^CG
SacII 796 CCGCGG CCGC^GG
ScaI 5178 AGTACT AGT^ACT
SfiI 841 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 714 CGCCGGTG C[GA]^CCGG[CT]G
SspI 4756 AATATT AAT^ATT
Tth111I 3392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5150 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 481 GACGTC GACGT^C
2653 GACGTC
AflIII 1435 ACATGT A^C[GA][CT]GT
5281 ACACGT
BbeI 625 GGCGCC GGCGC^C
3070 GGCGCC
BlpI 606 GCTCAGC GC^T[ACGT]AGC
1278 GCTGAGC
Bpu1102I 606 GCTCAGC GC^T[ACGT]AGC
1278 GCTGAGC
BsaAI 2019 TACGTG [CT]AC^GT[GA]
5282 CACGTG
BsaWI 5056 TCCGGA [AT]^CCGG[AT]
5393 ACCGGT
BstEII 366 GGTGACC G^GT[ACGT]ACC
387 GGTGACC
BstPI 366 GGTGACC G^GT[ACGT]ACC
387 GGTGACC
BstYI 1360 AGATCC [GA]^GATC[CT]
2324 GGATCC
CelII 606 GCTCAGC GC^T[ACGT]AGC
1278 GCTGAGC
EagI 807 CGGCCG C^GGCCG
1259 CGGCCG
EclXI 807 CGGCCG C^GGCCG
1259 CGGCCG
Eco52I 807 CGGCCG C^GGCCG
1259 CGGCCG
EcoO65I 366 GGTGACC G^GT[ACGT]ACC
387 GGTGACC
EcoT22I 2796 ATGCAT ATGCA^T
5196 ATGCAT
HaeII 625 GGCGCC [GA]GCGC^[CT]
3070 GGCGCC
KasI 625 GGCGCC G^GCGCC
3070 GGCGCC
MflI 1360 AGATCC [GA]^GATC[CT]
2324 GGATCC
NarI 625 GGCGCC GG^CGCC
3070 GGCGCC
NsiI 2796 ATGCAT ATGCA^T
5196 ATGCAT
SfoI 625 GGCGCC GGC^GCC
3070 GGCGCC
SmaI 3897 CCCGGG CCC^GGG
4976 CCCGGG
SphI 3294 GCATGC GCATG^C
3844 GCATGC
XhoII 1360 AGATCC [GA]^GATC[CT]
2324 GGATCC
XmaCI 3897 CCCGGG C^CCGGG
4976 CCCGGG
XmaI 3897 CCCGGG C^CCGGG
4976 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI