##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII BamHI BciVI BclI
BsaBI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
FbaI FspI KpnI MamI MluI
MroI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 2308 GGCGCC G[GA]^CG[CT]C
AflII 5042 CTTAAG C^TTAAG
AhdI 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1278 AGCGCT AGC^GCT
Asp700I 2899 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2308 GGCGCC GGCGC^C
BbsI 3669 GAAGAC GAAGAC(2/6)
BcgI 542 GCAGGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5042 CTTAAG C^TTAAG
BglII 419 AGATCT A^GATCT
BpuAI 3669 GAAGAC GAAGAC(2/6)
BsaHI 2308 GGCGCC G[GA]^CG[CT]C
BsaWI 592 ACCGGA [AT]^CCGG[AT]
BsiEI 1203 CGGCCG CG[GA][CT]^CG
BsmBI 4919 CGTCTC CGTCTC(1/5)
BsmI 3095 GAATGC GAATGC(1/-1)
BspHI 4168 TCATGA T^CATGA
BsrDI 3789 GCAATG GCAATG(2/0)
BssSI 1294 CACGAG CACGAG(-5/-1)
EagI 1203 CGGCCG C^GGCCG
Eam1105I 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1203 CGGCCG C^GGCCG
Eco47III 1278 AGCGCT AGC^GCT
Eco52I 1203 CGGCCG C^GGCCG
EcoT22I 3097 ATGCAT ATGCA^T
Hin1I 2308 GGCGCC G[GA]^CG[CT]C
HindIII 4347 AAGCTT A^AGCTT
HpaI 4943 GTTAAC GTT^AAC
KasI 2308 GGCGCC G^GCGCC
KspI 37 CCGCGG CCGC^GG
NarI 2308 GGCGCC GG^CGCC
NsiI 3097 ATGCAT ATGCA^T
PflFI 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4168 TCATGA T^CATGA
SacII 37 CCGCGG CCGC^GG
SalI 4279 GTCGAC G^TCGAC
SfoI 2308 GGCGCC GGC^GCC
SmaI 1327 CCCGGG CCC^GGG
SphI 3905 GCATGC GCATG^C
SspI 5448 AATATT AAT^ATT
Tth111I 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4806 TCTAGA T^CTAGA
XmaCI 1327 CCCGGG C^CCGGG
XmaI 1327 CCCGGG C^CCGGG
XmnI 2899 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 4279 GTCGAC GT^[AC][GT]AC
4752 GTCTAC
AcsI 3493 AAATTT [GA]^AATT[CT]
4795 AAATTC
ApaI 1102 GGGCCC GGGCC^C
4239 GGGCCC
ApoI 3493 AAATTT [GA]^AATT[CT]
4795 AAATTC
BbvCI 3514 CCTCAGC CCTCAGC(-5/-2)
531 GCTGAGG
BspLU11I 5291 ACATGT A^CATGT
5371 ACATGT
BsrBI 646 GAGCGG CCGCTC(-3/-3)
2404 GAGCGG
DraIII 631 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3546 CACCAGGTG
FseI 1137 GGCCGGCC GGCCGG^CC
1200 GGCCGGCC
MfeI 3425 CAATTG C^AATTG
5197 CAATTG
MunI 3425 CAATTG C^AATTG
5197 CAATTG
NaeI 1138 GCCGGC GCC^GGC
1201 GCCGGC
NcoI 3355 CCATGG C^CATGG
4967 CCATGG
NgoMIV 1138 GCCGGC G^CCGGC
1201 GCCGGC
SapI 612 GAAGAGC GCTCTTC(1/4)
3646 GCTCTTC
SexAI 3547 ACCAGGT A^CC[AT]GGT
3948 ACCAGGT
StuI 28 AGGCCT AGG^CCT
2884 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbrPI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII