################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AviII BamHI BciVI BclI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRI EcoRV FbaI FspI KpnI MamI MluI MroI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 2308 GGCGCC G[GA]^CG[CT]C AflII 5042 CTTAAG C^TTAAG AhdI 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1278 AGCGCT AGC^GCT Asp700I 2899 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2308 GGCGCC GGCGC^C BbsI 3669 GAAGAC GAAGAC(2/6) BcgI 542 GCAGGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 5042 CTTAAG C^TTAAG BglII 419 AGATCT A^GATCT BpuAI 3669 GAAGAC GAAGAC(2/6) BsaHI 2308 GGCGCC G[GA]^CG[CT]C BsaWI 592 ACCGGA [AT]^CCGG[AT] BsiEI 1203 CGGCCG CG[GA][CT]^CG BsmBI 4919 CGTCTC CGTCTC(1/5) BsmI 3095 GAATGC GAATGC(1/-1) BspHI 4168 TCATGA T^CATGA BsrDI 3789 GCAATG GCAATG(2/0) BssSI 1294 CACGAG CACGAG(-5/-1) EagI 1203 CGGCCG C^GGCCG Eam1105I 4912 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1203 CGGCCG C^GGCCG Eco47III 1278 AGCGCT AGC^GCT Eco52I 1203 CGGCCG C^GGCCG EcoT22I 3097 ATGCAT ATGCA^T Hin1I 2308 GGCGCC G[GA]^CG[CT]C HindIII 4347 AAGCTT A^AGCTT HpaI 4943 GTTAAC GTT^AAC KasI 2308 GGCGCC G^GCGCC KspI 37 CCGCGG CCGC^GG NarI 2308 GGCGCC GG^CGCC NsiI 3097 ATGCAT ATGCA^T PflFI 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 4168 TCATGA T^CATGA SacII 37 CCGCGG CCGC^GG SalI 4279 GTCGAC G^TCGAC SfoI 2308 GGCGCC GGC^GCC SmaI 1327 CCCGGG CCC^GGG SphI 3905 GCATGC GCATG^C SspI 5448 AATATT AAT^ATT Tth111I 3563 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4806 TCTAGA T^CTAGA XmaCI 1327 CCCGGG C^CCGGG XmaI 1327 CCCGGG C^CCGGG XmnI 2899 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 4279 GTCGAC GT^[AC][GT]AC 4752 GTCTAC AcsI 3493 AAATTT [GA]^AATT[CT] 4795 AAATTC ApaI 1102 GGGCCC GGGCC^C 4239 GGGCCC ApoI 3493 AAATTT [GA]^AATT[CT] 4795 AAATTC BbvCI 3514 CCTCAGC CCTCAGC(-5/-2) 531 GCTGAGG BspLU11I 5291 ACATGT A^CATGT 5371 ACATGT BsrBI 646 GAGCGG CCGCTC(-3/-3) 2404 GAGCGG DraIII 631 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3546 CACCAGGTG FseI 1137 GGCCGGCC GGCCGG^CC 1200 GGCCGGCC MfeI 3425 CAATTG C^AATTG 5197 CAATTG MunI 3425 CAATTG C^AATTG 5197 CAATTG NaeI 1138 GCCGGC GCC^GGC 1201 GCCGGC NcoI 3355 CCATGG C^CATGG 4967 CCATGG NgoMIV 1138 GCCGGC G^CCGGC 1201 GCCGGC SapI 612 GAAGAGC GCTCTTC(1/4) 3646 GCTCTTC SexAI 3547 ACCAGGT A^CC[AT]GGT 3948 ACCAGGT StuI 28 AGGCCT AGG^CCT 2884 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI AspHI AvaI AvaII AvrII BalI BanI BanII BbrPI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII