##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BglI
BlnI BsaWI BseAI BsiWI BspEI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FseI FspI
HindIII HpaI KasI KspI MluI
MroI NarI NcoI NotI NruI
NspV PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2311 GGTACC G^GTACC
AccI 3802 GTCTAC GT^[AC][GT]AC
AcyI 575 GGCGTC G[GA]^CG[CT]C
AflII 4606 CTTAAG C^TTAAG
Alw44I 2400 GTGCAC G^TGCAC
ApaLI 2400 GTGCAC G^TGCAC
Asp700I 3709 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2311 GGTACC G^GTACC
BanI 2311 GGTACC G^G[CT][GA]CC
BanII 1714 GAGCCC G[GA]GC[CT]^C
BfrI 4606 CTTAAG C^TTAAG
BlpI 4075 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4075 GCTAAGC GC^T[ACGT]AGC
BsaAI 4624 TACGTG [CT]AC^GT[GA]
BsaHI 575 GGCGTC G[GA]^CG[CT]C
BsmBI 2907 CGTCTC CGTCTC(1/5)
BspDI 2528 ATCGAT AT^CGAT
BsrBI 1566 GAGCGG CCGCTC(-3/-3)
BsrFI 439 GCCGGC [GA]^CCGG[CT]
BssHII 5018 GCGCGC G^CGCGC
BssSI 512 CACGAG CACGAG(-5/-1)
CelII 4075 GCTAAGC GC^T[ACGT]AGC
Cfr10I 439 GCCGGC [GA]^CCGG[CT]
ClaI 2528 ATCGAT AT^CGAT
DraII 1731 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 4819 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1731 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 419 CCCGC CCCGC(4/6)
HaeII 410 GGCGCT [GA]GCGC^[CT]
Hin1I 575 GGCGTC G[GA]^CG[CT]C
HincII 982 GTTGAC GT[CT]^[GA]AC
HindII 982 GTTGAC GT[CT]^[GA]AC
KpnI 2311 GGTACC GGTAC^C
MfeI 725 CAATTG C^AATTG
MunI 725 CAATTG C^AATTG
NaeI 439 GCCGGC GCC^GGC
NgoMIV 439 GCCGGC G^CCGGC
NheI 4419 GCTAGC G^CTAGC
PacI 3701 TTAATTAA TTAAT^TAA
PaeR7I 647 CTCGAG C^TCGAG
PpuMI 1731 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 5207 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2530 CGATCG CGAT^CG
SapI 3115 GAAGAGC GCTCTTC(1/4)
SspI 2484 AATATT AAT^ATT
StuI 1261 AGGCCT AGG^CCT
XbaI 4832 TCTAGA T^CTAGA
XcmI 3679 CCAAATGTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 647 CTCGAG C^TCGAG
XmnI 3709 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 946 CGCG CG^CG
5019 CGCG
AflIII 2077 ACATGT A^C[GA][CT]GT
3539 ACATGT
AseI 861 ATTAAT AT^TAAT
3431 ATTAAT
AsnI 861 ATTAAT AT^TAAT
3431 ATTAAT
AspHI 2081 GTGCTC G[AT]GC[AT]^C
2400 GTGCAC
AvaI 647 CTCGAG C^[CT]CG[GA]G
2469 CTCGGG
BbsI 3055 GAAGAC GAAGAC(2/6)
3419 GAAGAC
BciVI 2883 GGATAC GTATCC(6/5)
2703 GTATCC
BclI 1899 TGATCA T^GATCA
5183 TGATCA
BcnI 407 CCCGG CC^[GC]GG
1094 CCGGG
BglII 2247 AGATCT A^GATCT
4874 AGATCT
BpuAI 3055 GAAGAC GAAGAC(2/6)
3419 GAAGAC
BsaI 3092 GAGACC GGTCTC(1/5)
299 GGTCTC
BsgI 1627 GTGCAG GTGCAG(16/14)
2165 GTGCAG
BsiHKAI 2081 GTGCTC G[AT]GC[AT]^C
2400 GTGCAC
BsmI 1639 GCATTC GAATGC(1/-1)
5469 GCATTC
BsoBI 647 CTCGAG C^[CT]CG[GA]G
2469 CTCGGG
Bsp1407I 5001 TGTACA T^GTACA
5233 TGTACA
BspLU11I 2077 ACATGT A^CATGT
3539 ACATGT
BspMI 2094 GCAGGT ACCTGC(4/8)
2216 GCAGGT
BsrGI 5001 TGTACA T^GTACA
5233 TGTACA
BstUI 946 CGCG CG^CG
5019 CGCG
BstXI 3011 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3155 CCACCCTTCTGG
DraI 3749 TTTAAA TTT^AAA
4037 TTTAAA
EcoNI 1723 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3159 CCTTCTGGAGG
EcoRV 2623 GATATC GAT^ATC
2799 GATATC
FbaI 1899 TGATCA T^GATCA
5183 TGATCA
HgaI 20 GACGC GACGC(5/10)
576 GCGTC
MvnI 946 CGCG CG^CG
5019 CGCG
NciI 407 CCCGG CC^[GC]GG
1094 CCGGG
NdeI 1043 CATATG CA^TATG
1417 CATATG
PshBI 861 ATTAAT AT^TAAT
3431 ATTAAT
PstI 884 CTGCAG CTGCA^G
1312 CTGCAG
ScaI 472 AGTACT AGT^ACT
1128 AGTACT
SspBI 5001 TGTACA T^GTACA
5233 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BbvI BfaI
BmyI BpmI BsaBI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII