##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII AvrII BbeI
BbvCI BfrI BglI BlnI BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HaeII Hin1I KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
PinAI PmeI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 23 CGCG CG^CG
AccIII 1198 TCCGGA T^CCGGA
AocI 3131 CCTAAGG CC^T[ACGT]AGG
ApaI 1695 GGGCCC GGGCC^C
AspI 4044 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 382 TGGCCA TGG^CCA
BbrPI 3191 CACGTG CAC^GTG
BcgI 2503 GCATCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1605 GTATCC GTATCC(6/5)
BcnI 50 CCCGG CC^[GC]GG
BglII 1277 AGATCT A^GATCT
BlpI 3101 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3101 GCTCAGC GC^T[ACGT]AGC
BsaAI 3191 CACGTG [CT]AC^GT[GA]
BsaI 4441 GGTCTC GGTCTC(1/5)
BsaWI 1198 TCCGGA [AT]^CCGG[AT]
BseAI 1198 TCCGGA T^CCGGA
BspEI 1198 TCCGGA T^CCGGA
BspMI 3227 GCAGGT ACCTGC(4/8)
BsrBI 27 CCGCTC CCGCTC(-3/-3)
BsrDI 4350 CATTGC GCAATG(2/0)
BstUI 23 CGCG CG^CG
Bsu36I 3131 CCTAAGG CC^T[ACGT]AGG
CelII 3101 GCTCAGC GC^T[ACGT]AGC
EaeI 382 TGGCCA [CT]^GGCC[GA]
Eco81I 3131 CCTAAGG CC^T[ACGT]AGG
HgaI 1511 GCGTC GACGC(5/10)
HpaI 2547 GTTAAC GTT^AAC
MluNI 382 TGGCCA TGG^CCA
MroI 1198 TCCGGA T^CCGGA
MscI 382 TGGCCA TGG^CCA
MvnI 23 CGCG CG^CG
NciI 50 CCCGG CC^[GC]GG
NcoI 4490 CCATGG C^CATGG
NdeI 4145 CATATG CA^TATG
PacI 4405 TTAATTAA TTAAT^TAA
PaeR7I 5129 CTCGAG C^TCGAG
PflFI 4044 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4682 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3191 CACGTG CAC^GTG
PmlI 3191 CACGTG CAC^GTG
PshAI 467 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 830 GAGCTC GAGCT^C
SapI 939 GAAGAGC GCTCTTC(1/4)
SpeI 1446 ACTAGT A^CTAGT
SphI 3697 GCATGC GCATG^C
SspI 4989 AATATT AAT^ATT
SwaI 5089 ATTTAAAT ATTT^AAAT
Tth111I 4044 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4682 CCAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5129 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2044 GTAGAC GT^[AC][GT]AC
2470 GTAGAC
AclI 353 AACGTT AA^CGTT
3535 AACGTT
AseI 3426 ATTAAT AT^TAAT
3935 ATTAAT
AsnI 3426 ATTAAT AT^TAAT
3935 ATTAAT
AspHI 830 GAGCTC G[AT]GC[AT]^C
2691 GTGCTC
BamHI 1 GGATCC G^GATCC
4065 GGATCC
BanI 456 GGTGCC G^G[CT][GA]CC
3492 GGCACC
BanII 830 GAGCTC G[GA]GC[CT]^C
1695 GGGCCC
BclI 842 TGATCA T^GATCA
2344 TGATCA
BsaBI 3255 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4828 GATAACCATC
BsgI 1399 GTGCAG GTGCAG(16/14)
5122 GTGCAG
BsiHKAI 830 GAGCTC G[AT]GC[AT]^C
2691 GTGCTC
BsmI 2297 GAATGC GAATGC(1/-1)
3021 GAATGC
BstBI 748 TTCGAA TT^CGAA
1382 TTCGAA
BstXI 553 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1211 CCACCCCAGTGG
CfoI 24 GCGC GCG^C
4260 GCGC
DsaI 2606 CCGTGG C^C[GA][CT]GG
4490 CCATGG
EcoNI 1680 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4877 CCTGCATAAGG
EcoT14I 1726 CCAAGG C^C[AT][AT]GG
4490 CCATGG
EcoT22I 4302 ATGCAT ATGCA^T
4753 ATGCAT
FauI 21 CCCGC CCCGC(4/6)
60 CCCGC
FbaI 842 TGATCA T^GATCA
2344 TGATCA
HapII 51 CCGG C^CGG
1199 CCGG
HhaI 24 GCGC GCG^C
4260 GCGC
HinP1I 24 GCGC G^CGC
4260 GCGC
HincII 2547 GTTAAC GT[CT]^[GA]AC
4050 GTCAAC
HindII 2547 GTTAAC GT[CT]^[GA]AC
4050 GTCAAC
HpaII 51 CCGG C^CGG
1199 CCGG
MamI 3255 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4828 GATAACCATC
MspA1I 93 CAGCTG C[AC]G^C[GT]G
4477 CAGCTG
MspI 51 CCGG C^CGG
1199 CCGG
NsiI 4302 ATGCAT ATGCA^T
4753 ATGCAT
NspV 748 TTCGAA TT^CGAA
1382 TTCGAA
PpuMI 742 AGGACCT [GA]G^G[AT]CC[CT]
2599 AGGACCT
PshBI 3426 ATTAAT AT^TAAT
3935 ATTAAT
Psp1406I 353 AACGTT AA^CGTT
3535 AACGTT
PvuII 93 CAGCTG CAG^CTG
4477 CAGCTG
SexAI 1849 ACCAGGT A^CC[AT]GGT
5176 ACCTGGT
SfuI 748 TTCGAA TT^CGAA
1382 TTCGAA
SmlI 570 CTCAAG C^T[CT][GA]AG
5129 CTCGAG
StyI 1726 CCAAGG C^C[AT][AT]GG
4490 CCATGG
XbaI 1004 TCTAGA T^CTAGA
3800 TCTAGA
XcmI 1585 CCATGCCAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2283 CCACTCATCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRI EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI