##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BfrI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I FseI FspI HaeII Hin1I
HindIII KasI KpnI KspI MamI
MluI MroI MvnI NarI NcoI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 184 GTCTAC GT^[AC][GT]AC
AclI 5082 AACGTT AA^CGTT
AhdI 1595 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1031 GTGCAC G^TGCAC
ApaI 3046 GGGCCC GGGCC^C
ApaLI 1031 GTGCAC G^TGCAC
AspEI 1595 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1392 CCCGGG C^[CT]CG[GA]G
BbrPI 1054 CACGTG CAC^GTG
BcgI 1374 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 798 GTATCC GTATCC(6/5)
BclI 1114 TGATCA T^GATCA
BsaAI 1054 CACGTG [CT]AC^GT[GA]
BsgI 1136 CTGCAC GTGCAG(16/14)
BsiEI 3176 CGACCG CG[GA][CT]^CG
BsmBI 1298 CGTCTC CGTCTC(1/5)
BsoBI 1392 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4509 TGTACA T^GTACA
BspHI 2482 TCATGA T^CATGA
BsrGI 4509 TGTACA T^GTACA
Eam1105I 1595 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3297 CCTTTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1814 GATATC GAT^ATC
FauI 2369 GCGGG CCCGC(4/6)
FbaI 1114 TGATCA T^GATCA
HpaI 1505 GTTAAC GTT^AAC
MfeI 2656 CAATTG C^AATTG
MspA1I 3232 CAGCTG C[AC]G^C[GT]G
MunI 2656 CAATTG C^AATTG
NaeI 2644 GCCGGC GCC^GGC
NdeI 2243 CATATG CA^TATG
NgoMIV 2644 GCCGGC G^CCGGC
PmaCI 1054 CACGTG CAC^GTG
PmlI 1054 CACGTG CAC^GTG
Psp1406I 5082 AACGTT AA^CGTT
PvuII 3232 CAGCTG CAG^CTG
RcaI 2482 TCATGA T^CATGA
SacI 5058 GAGCTC GAGCT^C
SmaI 1392 CCCGGG CCC^GGG
SphI 2648 GCATGC GCATG^C
SspBI 4509 TGTACA T^GTACA
TaqI 4755 TCGA T^CGA
TthHB8I 4755 TCGA T^CGA
XbaI 689 TCTAGA T^CTAGA
XmaCI 1392 CCCGGG C^CCGGG
XmaI 1392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 2452 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
4310 CAGCAACTG
AocI 2877 CCTGAGG CC^T[ACGT]AGG
3489 CCTCAGG
Asp700I 122 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3308 GAATGCATTC
BamHI 3695 GGATCC G^GATCC
4578 GGATCC
BglI 2248 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3708 GCCCAGTGGGC
BspLU11I 1662 ACATGT A^CATGT
3087 ACATGT
BstAPI 3555 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4274 GCACGCTATGC
BstEII 1592 GGTGACC G^GT[ACGT]ACC
4866 GGTTACC
BstPI 1592 GGTGACC G^GT[ACGT]ACC
4866 GGTTACC
BstXI 1218 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2948 CCAAGCTAATGG
Bsu36I 2877 CCTGAGG CC^T[ACGT]AGG
3489 CCTCAGG
CfoI 702 GCGC GCG^C
2357 GCGC
Eco81I 2877 CCTGAGG CC^T[ACGT]AGG
3489 CCTCAGG
EcoO65I 1592 GGTGACC G^GT[ACGT]ACC
4866 GGTTACC
HgaI 1694 GACGC GACGC(5/10)
3205 GCGTC
HhaI 702 GCGC GCG^C
2357 GCGC
HinP1I 702 GCGC G^CGC
2357 GCGC
MslI 1118 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4637 CATGCTTATG
ScaI 3902 AGTACT AGT^ACT
3953 AGTACT
XmnI 122 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3308 GAATGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AspI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XcmI
XhoII