##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI BamHI
BanI BbeI BbrPI BcgI BclI
BfrI BsaAI BsaHI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraIII DrdI Eco47III EcoNI
EcoO65I EcoRI FbaI FseI HaeII
Hin1I KasI KpnI KspI MluI
MroI MvnI NarI NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 33 ACACGT A^C[GA][CT]GT
AhdI 3752 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4002 CCTAAGG CC^T[ACGT]AGG
ApaI 4338 GGGCCC GGGCC^C
AseI 3630 ATTAAT AT^TAAT
AsnI 3630 ATTAAT AT^TAAT
AspEI 3752 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4341 CCCGGG C^[CT]CG[GA]G
AviII 1019 TGCGCA TGC^GCA
AvrII 46 CCTAGG C^CTAGG
BbsI 891 GAAGAC GAAGAC(2/6)
BbvCI 711 CCTCAGC CCTCAGC(-5/-2)
BciVI 4828 GGATAC GTATCC(6/5)
BglI 1654 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 46 CCTAGG C^CTAGG
BlpI 304 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 304 GCTCAGC GC^T[ACGT]AGC
BpuAI 891 GAAGAC GAAGAC(2/6)
BsaBI 1173 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3166 GAGACC GGTCTC(1/5)
BsoBI 4341 CCCGGG C^[CT]CG[GA]G
BsrBI 924 GAGCGG CCGCTC(-3/-3)
BstXI 860 CCAATTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4002 CCTAAGG CC^T[ACGT]AGG
CelII 304 GCTCAGC GC^T[ACGT]AGC
EagI 61 CGGCCG C^GGCCG
Eam1105I 3752 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 61 CGGCCG C^GGCCG
Eco52I 61 CGGCCG C^GGCCG
Eco81I 4002 CCTAAGG CC^T[ACGT]AGG
FspI 1019 TGCGCA TGC^GCA
MamI 1173 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2185 CAGCTG C[AC]G^C[GT]G
PacI 4579 TTAATTAA TTAAT^TAA
PflMI 1060 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 999 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3630 ATTAAT AT^TAAT
PvuI 1178 CGATCG CGAT^CG
PvuII 2185 CAGCTG CAG^CTG
SapI 3113 GCTCTTC GCTCTTC(1/4)
SmaI 4341 CCCGGG CCC^GGG
SmlI 886 CTCAAG C^T[CT][GA]AG
SpeI 3611 ACTAGT A^CTAGT
Van91I 1060 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4341 CCCGGG C^CCGGG
XmaI 4341 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 778 GTCTAC GT^[AC][GT]AC
2848 GTCTAC
Asp700I 544 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2433 GAAAACATTC
AspHI 176 GTGCTC G[AT]GC[AT]^C
4250 GTGCTC
BalI 1652 TGGCCA TGG^CCA
5412 TGGCCA
BglII 2930 AGATCT A^GATCT
4348 AGATCT
BsgI 1764 CTGCAC GTGCAG(16/14)
1579 GTGCAG
BsiEI 61 CGGCCG CG[GA][CT]^CG
1178 CGATCG
BsiHKAI 176 GTGCTC G[AT]GC[AT]^C
4250 GTGCTC
Bsp1407I 4590 TGTACA T^GTACA
5061 TGTACA
BspHI 506 TCATGA T^CATGA
552 TCATGA
BsrDI 599 CATTGC GCAATG(2/0)
822 CATTGC
BsrFI 59 GCCGGC [GA]^CCGG[CT]
2225 GCCGGC
BsrGI 4590 TGTACA T^GTACA
5061 TGTACA
Cfr10I 59 GCCGGC [GA]^CCGG[CT]
2225 GCCGGC
DraII 999 GGGACCT [GA]G^G[ACGT]CC[CT]
4337 AGGGCCC
EcoO109I 999 GGGACCT [GA]G^G[ACGT]CC[CT]
4337 AGGGCCC
FauI 21 CCCGC CCCGC(4/6)
4 GCGGG
HgaI 1039 GACGC GACGC(5/10)
3988 GACGC
MfeI 687 CAATTG C^AATTG
773 CAATTG
MluNI 1652 TGGCCA TGG^CCA
5412 TGGCCA
MscI 1652 TGGCCA TGG^CCA
5412 TGGCCA
MunI 687 CAATTG C^AATTG
773 CAATTG
NaeI 59 GCCGGC GCC^GGC
2225 GCCGGC
NcoI 474 CCATGG C^CATGG
3452 CCATGG
NgoMIV 59 GCCGGC G^CCGGC
2225 GCCGGC
RcaI 506 TCATGA T^CATGA
552 TCATGA
SspBI 4590 TGTACA T^GTACA
5061 TGTACA
TaqI 1950 TCGA T^CGA
2264 TCGA
TthHB8I 1950 TCGA T^CGA
2264 TCGA
XbaI 2888 TCTAGA T^CTAGA
5343 TCTAGA
XmnI 544 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2433 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoRV EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII