##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AocI Aor51HI ApaI AscI
Asp718I AspI AviII AvrII BalI
BamHI BbeI BcgI BclI BglI
BlnI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspMI BsrBI BssHII BssSI BstAPI
BstUI Bsu36I CfoI ClaI CpoI
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI PacI PflFI PinAI
PmeI PpuMI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 2612 AACGTT AA^CGTT
AhdI 1506 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1101 ATTAAT AT^TAAT
AsnI 1101 ATTAAT AT^TAAT
AspEI 1506 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2539 CTCGAG C^[CT]CG[GA]G
BanI 2909 GGTGCC G^G[CT][GA]CC
BbrPI 3492 CACGTG CAC^GTG
BbvCI 307 CCTCAGC CCTCAGC(-5/-2)
BciVI 4573 GTATCC GTATCC(6/5)
BcnI 3398 CCCGG CC^[GC]GG
BglII 1436 AGATCT A^GATCT
BlpI 1524 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1524 GCTCAGC GC^T[ACGT]AGC
BsoBI 2539 CTCGAG C^[CT]CG[GA]G
BspLU11I 3745 ACATGT A^CATGT
BsrFI 2207 GCCGGT [GA]^CCGG[CT]
Bst1107I 4464 GTATAC GTA^TAC
BstBI 1687 TTCGAA TT^CGAA
BstXI 4972 CCAGTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4464 GTATAC GTA^TAC
CelII 1524 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2207 GCCGGT [GA]^CCGG[CT]
DraII 2946 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 162 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1244 CCGTGG C^C[GA][CT]GG
Eam1105I 1506 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2946 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2118 GATATC GAT^ATC
EcoT22I 4147 ATGCAT ATGCA^T
FauI 2618 GCGGG CCCGC(4/6)
HpaI 4067 GTTAAC GTT^AAC
MwoI 504 GCCAATGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 3398 CCCGG CC^[GC]GG
NsiI 4147 ATGCAT ATGCA^T
NspV 1687 TTCGAA TT^CGAA
PaeR7I 2539 CTCGAG C^TCGAG
PmaCI 3492 CACGTG CAC^GTG
PmlI 3492 CACGTG CAC^GTG
PshBI 1101 ATTAAT AT^TAAT
Psp1406I 2612 AACGTT AA^CGTT
SacI 41 GAGCTC GAGCT^C
ScaI 4306 AGTACT AGT^ACT
SexAI 4368 ACCTGGT A^CC[AT]GGT
SfuI 1687 TTCGAA TT^CGAA
SphI 4133 GCATGC GCATG^C
SwaI 4661 ATTTAAAT ATTT^AAAT
XbaI 1982 TCTAGA T^CTAGA
XhoI 2539 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3419 GTAGAC GT^[AC][GT]AC
4464 GTATAC
AflII 996 CTTAAG C^TTAAG
3833 CTTAAG
AflIII 1938 ACACGT A^C[GA][CT]GT
3745 ACATGT
Alw44I 3239 GTGCAC G^TGCAC
4595 GTGCAC
ApaLI 3239 GTGCAC G^TGCAC
4595 GTGCAC
AvaII 564 GGTCC G^G[AT]CC
3839 GGACC
BanII 41 GAGCTC G[GA]GC[CT]^C
3724 GGGCTC
BfrI 996 CTTAAG C^TTAAG
3833 CTTAAG
BsaAI 1945 CACGTA [CT]AC^GT[GA]
3492 CACGTG
BsaBI 987 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2308 GATCTCCATC
BseRI 44 CTCCTC GAGGAG(10/8)
2691 GAGGAG
BsgI 2365 CTGCAC GTGCAG(16/14)
793 GTGCAG
BsmFI 3506 GGGAC GGGAC(10/14)
3838 GGGAC
BsmI 1352 GAATGC GAATGC(1/-1)
2657 GAATGC
Bsp1407I 4532 TGTACA T^GTACA
5007 TGTACA
BspHI 521 TCATGA T^CATGA
989 TCATGA
BsrGI 4532 TGTACA T^GTACA
5007 TGTACA
BstEII 2210 GGTGACC G^GT[ACGT]ACC
3430 GGTGACC
BstPI 2210 GGTGACC G^GT[ACGT]ACC
3430 GGTGACC
EcoO65I 2210 GGTGACC G^GT[ACGT]ACC
3430 GGTGACC
HaeIII 2206 GGCC GG^CC
2947 GGCC
HapII 2208 CCGG C^CGG
3399 CCGG
HincII 2194 GTCAAC GT[CT]^[GA]AC
4067 GTTAAC
HindII 2194 GTCAAC GT[CT]^[GA]AC
4067 GTTAAC
HpaII 2208 CCGG C^CGG
3399 CCGG
MamI 987 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2308 GATCTCCATC
MspI 2208 CCGG C^CGG
3399 CCGG
NdeI 3015 CATATG CA^TATG
4651 CATATG
NheI 1143 GCTAGC G^CTAGC
2286 GCTAGC
PstI 114 CTGCAG CTGCA^G
2446 CTGCAG
RcaI 521 TCATGA T^CATGA
989 TCATGA
SspBI 4532 TGTACA T^GTACA
5007 TGTACA
TaqI 1688 TCGA T^CGA
2540 TCGA
TthHB8I 1688 TCGA T^CGA
2540 TCGA
XcmI 2134 CCAAAGATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4969 CCACCAGTATAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII XmnI