##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AvrII BanI BbeI BglI BglII
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BspDI BspEI
BsrBI BssHII BstEII BstPI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NcoI NgoMIV NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 3015 AACGTT AA^CGTT
AflII 2964 CTTAAG C^TTAAG
AlwNI 4698 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 2160 TGCGCA TGC^GCA
BamHI 4140 GGATCC G^GATCC
BanII 3517 GAGCCC G[GA]GC[CT]^C
BbrPI 2952 CACGTG CAC^GTG
BcgI 878 GCATGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4609 GGATAC GTATCC(6/5)
BclI 2676 TGATCA T^GATCA
BfrI 2964 CTTAAG C^TTAAG
BsaAI 2952 CACGTG [CT]AC^GT[GA]
BsmBI 2124 CGTCTC CGTCTC(1/5)
Bsp1407I 4080 TGTACA T^GTACA
BspLU11I 3728 ACATGT A^CATGT
BspMI 4535 GCAGGT ACCTGC(4/8)
BsrFI 1796 ACCGGC [GA]^CCGG[CT]
BsrGI 4080 TGTACA T^GTACA
BssSI 1864 CTCGTG CACGAG(-5/-1)
BstBI 1041 TTCGAA TT^CGAA
Cfr10I 1796 ACCGGC [GA]^CCGG[CT]
DraIII 3068 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 2805 GATATC GAT^ATC
EcoT22I 2591 ATGCAT ATGCA^T
FbaI 2676 TGATCA T^GATCA
FspI 2160 TGCGCA TGC^GCA
HincII 2577 GTCAAC GT[CT]^[GA]AC
HindII 2577 GTCAAC GT[CT]^[GA]AC
MspA1I 4701 CAGCTG C[AC]G^C[GT]G
NdeI 2930 CATATG CA^TATG
NheI 1058 GCTAGC G^CTAGC
NsiI 2591 ATGCAT ATGCA^T
NspV 1041 TTCGAA TT^CGAA
PmaCI 2952 CACGTG CAC^GTG
PmlI 2952 CACGTG CAC^GTG
Psp1406I 3015 AACGTT AA^CGTT
PvuII 4701 CAGCTG CAG^CTG
ScaI 634 AGTACT AGT^ACT
SexAI 3182 ACCTGGT A^CC[AT]GGT
SfuI 1041 TTCGAA TT^CGAA
SphI 3935 GCATGC GCATG^C
SspBI 4080 TGTACA T^GTACA
SwaI 4842 ATTTAAAT ATTT^AAAT
XbaI 5052 TCTAGA T^CTAGA
XcmI 3313 CCATTACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1146 CGCG CG^CG
2248 CGCG
AvaI 2184 CTCGGG C^[CT]CG[GA]G
3359 CTCGGG
BbsI 918 GAAGAC GAAGAC(2/6)
5108 GAAGAC
BbvCI 1894 GCTGAGG CCTCAGC(-5/-2)
2192 GCTGAGG
BlpI 873 GCTGAGC GC^T[ACGT]AGC
5072 GCTGAGC
BmyI 258 GGGCAC G[AGT]GC[ACT]^C
3517 GAGCCC
Bpu1102I 873 GCTGAGC GC^T[ACGT]AGC
5072 GCTGAGC
BpuAI 918 GAAGAC GAAGAC(2/6)
5108 GAAGAC
BsoBI 2184 CTCGGG C^[CT]CG[GA]G
3359 CTCGGG
Bsp1286I 258 GGGCAC G[AGT]GC[ACT]^C
3517 GAGCCC
BspHI 2076 TCATGA T^CATGA
2679 TCATGA
Bst1107I 1436 GTATAC GTA^TAC
3898 GTATAC
BstUI 1146 CGCG CG^CG
2248 CGCG
BstXI 361 CCATTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3843 CCATGTGAGTGG
BstZ17I 1436 GTATAC GTA^TAC
3898 GTATAC
CelII 873 GCTGAGC GC^T[ACGT]AGC
5072 GCTGAGC
CfoI 2161 GCGC GCG^C
2249 GCGC
DsaI 46 CCGTGG C^C[GA][CT]GG
1165 CCGTGG
EcoNI 87 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1028 CCTGGGACAGG
EcoRI 271 GAATTC G^AATTC
5015 GAATTC
HhaI 2161 GCGC GCG^C
2249 GCGC
HinP1I 2161 GCGC G^CGC
2249 GCGC
MfeI 4148 CAATTG C^AATTG
5207 CAATTG
MunI 4148 CAATTG C^AATTG
5207 CAATTG
MvnI 1146 CGCG CG^CG
2248 CGCG
PstI 2365 CTGCAG CTGCA^G
3290 CTGCAG
RcaI 2076 TCATGA T^CATGA
2679 TCATGA
StuI 3749 AGGCCT AGG^CCT
4676 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AseI
AsnI AspI AvaII BalI BbvI
BcnI BfaI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII