##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AhdI AscI
AseI AsnI AspEI BciVI BclI
BsaBI BseAI BsiWI BsmI BspEI
BspHI Bst1107I BstBI BstZ17I CpoI
DraI Eam1105I EcoRV FbaI FseI
HpaI KspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 507 GGTACC G^GTACC
AflII 361 CTTAAG C^TTAAG
AgeI 510 ACCGGT A^CCGGT
Asp700I 3834 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 507 GGTACC G^GTACC
AspI 2768 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 763 TGCGCA TGC^GCA
BamHI 3211 GGATCC G^GATCC
BfrI 361 CTTAAG C^TTAAG
BsmBI 3096 CGTCTC CGTCTC(1/5)
Bsp1407I 4353 TGTACA T^GTACA
BspDI 4167 ATCGAT AT^CGAT
BsrBI 1009 GAGCGG CCGCTC(-3/-3)
BsrGI 4353 TGTACA T^GTACA
BstAPI 2117 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 4167 ATCGAT AT^CGAT
DrdI 3188 GACCACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 539 CGGCCG C^GGCCG
EclXI 539 CGGCCG C^GGCCG
Eco52I 539 CGGCCG C^GGCCG
EcoRI 5627 GAATTC G^AATTC
FspI 763 TGCGCA TGC^GCA
HincII 3460 GTTGAC GT[CT]^[GA]AC
HindII 3460 GTTGAC GT[CT]^[GA]AC
KpnI 507 GGTACC GGTAC^C
PaeR7I 313 CTCGAG C^TCGAG
PflFI 2768 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 510 ACCGGT A^CCGGT
PshAI 3862 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1221 CCTGCAGG CCTGCA^GG
SexAI 1751 ACCTGGT A^CC[AT]GGT
SpeI 2627 ACTAGT A^CTAGT
SphI 4241 GCATGC GCATG^C
Sse8387I 1221 CCTGCAGG CCTGCA^GG
SspBI 4353 TGTACA T^GTACA
Tth111I 2768 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 313 CTCGAG C^TCGAG
XmnI 3834 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbrPI 2165 CACGTG CAC^GTG
4670 CACGTG
BcgI 939 CGAAGCGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1230 CGAGGGACGTGC
BglII 4639 AGATCT A^GATCT
4758 AGATCT
BlpI 236 GCTGAGC GC^T[ACGT]AGC
1496 GCTCAGC
Bpu1102I 236 GCTGAGC GC^T[ACGT]AGC
1496 GCTCAGC
BsaI 4444 GAGACC GGTCTC(1/5)
603 GGTCTC
BsaWI 510 ACCGGT [AT]^CCGG[AT]
3035 ACCGGA
BsiEI 539 CGGCCG CG[GA][CT]^CG
931 CGACCG
BssSI 1345 CTCGTG CACGAG(-5/-1)
4349 CTCGTG
CelII 236 GCTGAGC GC^T[ACGT]AGC
1496 GCTCAGC
DraIII 3344 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5256 CACGAAGTG
EarI 3670 GAAGAG CTCTTC(1/4)
4849 GAAGAG
HindIII 3887 AAGCTT A^AGCTT
4777 AAGCTT
Ksp632I 3670 GAAGAG CTCTTC(1/4)
4849 GAAGAG
NdeI 1076 CATATG CA^TATG
4212 CATATG
PmaCI 2165 CACGTG CAC^GTG
4670 CACGTG
PmlI 2165 CACGTG CAC^GTG
4670 CACGTG
StuI 1310 AGGCCT AGG^CCT
2756 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrFI
BsrI BssHII BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI