##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Aor51HI AscI Asp718I AviII
BbrPI BcgI BciVI BclI BglI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I BsrFI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI DraIII DrdI Eco47III EcoO65I
EcoRV FbaI FseI FspI KpnI
MamI MluI MroI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflII 3259 CTTAAG C^TTAAG
AhdI 874 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4412 GTGCAC G^TGCAC
ApaI 275 GGGCCC GGGCC^C
ApaLI 4412 GTGCAC G^TGCAC
AseI 2890 ATTAAT AT^TAAT
AsnI 2890 ATTAAT AT^TAAT
Asp700I 3861 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 874 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2722 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
AvrII 1401 CCTAGG C^CTAGG
BamHI 3366 GGATCC G^GATCC
BbeI 526 GGCGCC GGCGC^C
BbvCI 2662 CCTCAGC CCTCAGC(-5/-2)
BfrI 3259 CTTAAG C^TTAAG
BlnI 1401 CCTAGG C^CTAGG
BsaAI 3239 TACGTG [CT]AC^GT[GA]
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsmBI 1986 CGTCTC CGTCTC(1/5)
BsoBI 125 CCCGGG C^[CT]CG[GA]G
BspHI 516 TCATGA T^CATGA
BsrBI 420 GAGCGG CCGCTC(-3/-3)
BstXI 2337 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 281 CGGTCCG CG^G[AT]CCG
EagI 41 CGGCCG C^GGCCG
Eam1105I 874 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoNI 4704 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 526 GGCGCC G^GCGCC
KspI 205 CCGCGG CCGC^GG
NarI 526 GGCGCC GG^CGCC
NdeI 3316 CATATG CA^TATG
PflFI 2722 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2890 ATTAAT AT^TAAT
RcaI 516 TCATGA T^CATGA
RsrII 281 CGGTCCG CG^G[AT]CCG
SacI 3254 GAGCTC GAGCT^C
SacII 205 CCGCGG CCGC^GG
ScaI 4695 AGTACT AGT^ACT
SfoI 526 GGCGCC GGC^GCC
SmaI 125 CCCGGG CCC^GGG
SmlI 3259 CTTAAG C^T[CT][GA]AG
SphI 3025 GCATGC GCATG^C
SwaI 1787 ATTTAAAT ATTT^AAAT
Tth111I 2722 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2693 CCAATAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 3861 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 745 GTCTAC GT^[AC][GT]AC
1553 GTCTAC
AocI 1432 CCTCAGG CC^T[ACGT]AGG
2444 CCTCAGG
BbsI 4816 GAAGAC GAAGAC(2/6)
4214 GTCTTC
BlpI 3005 GCTCAGC GC^T[ACGT]AGC
5329 GCTTAGC
Bpu1102I 3005 GCTCAGC GC^T[ACGT]AGC
5329 GCTTAGC
BpuAI 4816 GAAGAC GAAGAC(2/6)
4214 GTCTTC
BsmI 714 GAATGC GAATGC(1/-1)
5227 GAATGC
BssHII 33 GCGCGC G^CGCGC
250 GCGCGC
BstAPI 137 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
664 GCATTATCTGC
Bsu36I 1432 CCTCAGG CC^T[ACGT]AGG
2444 CCTCAGG
CelII 3005 GCTCAGC GC^T[ACGT]AGC
5329 GCTTAGC
EarI 2310 CTCTTC CTCTTC(1/4)
2778 CTCTTC
Eco81I 1432 CCTCAGG CC^T[ACGT]AGG
2444 CCTCAGG
EcoRI 314 GAATTC G^AATTC
1895 GAATTC
EcoT22I 761 ATGCAT ATGCA^T
1084 ATGCAT
HaeII 471 GGCGCT [GA]GCGC^[CT]
526 GGCGCC
HindIII 1007 AAGCTT A^AGCTT
4189 AAGCTT
HpaI 2947 GTTAAC GTT^AAC
3793 GTTAAC
Ksp632I 2310 CTCTTC CTCTTC(1/4)
2778 CTCTTC
NsiI 761 ATGCAT ATGCA^T
1084 ATGCAT
NspI 1686 ACATGC [GA]CATG^[CT]
3025 GCATGC
SexAI 2637 ACCAGGT A^CC[AT]GGT
3759 ACCAGGT
StuI 1346 AGGCCT AGG^CCT
3446 AGGCCT
TaqI 822 TCGA T^CGA
2561 TCGA
TthHB8I 822 TCGA T^CGA
2561 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII