##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbrPI BclI BfrI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FbaI FseI FspI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
SfiI SfuI SgrAI SpeI SphI
Sse8387I SspBI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 586 GGTACC G^GTACC
AcyI 4822 GGCGCC G[GA]^CG[CT]C
Alw44I 2600 GTGCAC G^TGCAC
AocI 1445 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3311 AGCGCT AGC^GCT
ApaLI 2600 GTGCAC G^TGCAC
Asp718I 586 GGTACC G^GTACC
BbeI 4822 GGCGCC GGCGC^C
BciVI 3093 GGATAC GTATCC(6/5)
BglII 935 AGATCT A^GATCT
BsaAI 4430 TACGTA [CT]AC^GT[GA]
BsaHI 4822 GGCGCC G[GA]^CG[CT]C
BsaWI 3455 ACCGGA [AT]^CCGG[AT]
BseRI 1413 GAGGAG GAGGAG(10/8)
BspHI 2918 TCATGA T^CATGA
BspLU11I 1192 ACATGT A^CATGT
BsrDI 3177 CATTGC GCAATG(2/0)
BssSI 272 CTCGTG CACGAG(-5/-1)
BstEII 3342 GGTGACC G^GT[ACGT]ACC
BstPI 3342 GGTGACC G^GT[ACGT]ACC
BstYI 935 AGATCT [GA]^GATC[CT]
Bsu36I 1445 CCTTAGG CC^T[ACGT]AGG
DraIII 3253 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3311 AGCGCT AGC^GCT
Eco81I 1445 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3342 GGTGACC G^GT[ACGT]ACC
EcoT22I 887 ATGCAT ATGCA^T
HgaI 2116 GCGTC GACGC(5/10)
Hin1I 4822 GGCGCC G[GA]^CG[CT]C
HincII 1993 GTTAAC GT[CT]^[GA]AC
HindII 1993 GTTAAC GT[CT]^[GA]AC
HpaI 1993 GTTAAC GTT^AAC
KasI 4822 GGCGCC G^GCGCC
KpnI 586 GGTACC GGTAC^C
KspI 3363 CCGCGG CCGC^GG
MflI 935 AGATCT [GA]^GATC[CT]
NarI 4822 GGCGCC GG^CGCC
NcoI 1527 CCATGG C^CATGG
NdeI 1740 CATATG CA^TATG
NsiI 887 ATGCAT ATGCA^T
PvuII 1291 CAGCTG CAG^CTG
RcaI 2918 TCATGA T^CATGA
SacII 3363 CCGCGG CCGC^GG
ScaI 3107 AGTACT AGT^ACT
SfoI 4822 GGCGCC GGC^GCC
SmaI 131 CCCGGG CCC^GGG
SnaBI 4430 TACGTA TAC^GTA
SspI 908 AATATT AAT^ATT
XbaI 2077 TCTAGA T^CTAGA
XcmI 1265 CCAGTCTCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 935 AGATCT [GA]^GATC[CT]
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 101 CGCG CG^CG
3364 CGCG
AflIII 1192 ACATGT A^C[GA][CT]GT
3353 ACGTGT
AspI 2435 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACCCTGTC
AvrII 1374 CCTAGG C^CTAGG
4370 CCTAGG
BbsI 3013 GAAGAC GAAGAC(2/6)
4126 GTCTTC
BglI 3473 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4114 GCCCTTGGGGC
BlnI 1374 CCTAGG C^CTAGG
4370 CCTAGG
BpuAI 3013 GAAGAC GAAGAC(2/6)
4126 GTCTTC
BsmI 493 GAATGC GAATGC(1/-1)
885 GAATGC
BstAPI 505 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3977 GCAGGTTGTGC
BstUI 101 CGCG CG^CG
3364 CGCG
HindIII 1433 AAGCTT A^AGCTT
1805 AAGCTT
MvnI 101 CGCG CG^CG
3364 CGCG
NspI 1192 ACATGT [GA]CATG^[CT]
1612 ACATGC
PflFI 2435 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACCCTGTC
SexAI 2210 ACCAGGT A^CC[AT]GGT
3254 ACCAGGT
StuI 1540 AGGCCT AGG^CCT
3146 AGGCCT
Tth111I 2435 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2473 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I