##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII BclI BfrI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
AclI 4516 AACGTT AA^CGTT
AflIII 121 ACGTGT A^C[GA][CT]GT
Alw44I 3632 GTGCAC G^TGCAC
AocI 2769 CCTCAGG CC^T[ACGT]AGG
ApaLI 3632 GTGCAC G^TGCAC
AspI 4031 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3515 CCTAGG C^CTAGG
BbeI 3234 GGCGCC GGCGC^C
BbvCI 2911 GCTGAGG CCTCAGC(-5/-2)
BciVI 3798 GTATCC GTATCC(6/5)
BglII 1051 AGATCT A^GATCT
BlnI 3515 CCTAGG C^CTAGG
BsmI 1436 GCATTC GAATGC(1/-1)
BstAPI 2336 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2769 CCTCAGG CC^T[ACGT]AGG
EagI 430 CGGCCG C^GGCCG
EclXI 430 CGGCCG C^GGCCG
Eco52I 430 CGGCCG C^GGCCG
Eco81I 2769 CCTCAGG CC^T[ACGT]AGG
EcoRI 2947 GAATTC G^AATTC
KasI 3234 GGCGCC G^GCGCC
MseI 1133 TTAA T^TAA
NarI 3234 GGCGCC GG^CGCC
PflFI 4031 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1841 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4516 AACGTT AA^CGTT
SacI 3540 GAGCTC GAGCT^C
SfiI 2045 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3234 GGCGCC GGC^GCC
SgrAI 2313 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 3506 CTTGAG C^T[CT][GA]AG
Tru9I 1133 TTAA T^TAA
Tth111I 4031 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccIII 4038 TCCGGA T^CCGGA
4521 TCCGGA
AcsI 2665 AAATTT [GA]^AATT[CT]
2947 GAATTC
ApaI 180 GGGCCC GGGCC^C
2480 GGGCCC
ApoI 2665 AAATTT [GA]^AATT[CT]
2947 GAATTC
BbrPI 120 CACGTG CAC^GTG
2331 CACGTG
BcgI 4877 CGAACGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
240 GCACTTCCCTCG
BseAI 4038 TCCGGA T^CCGGA
4521 TCCGGA
BspEI 4038 TCCGGA T^CCGGA
4521 TCCGGA
BssSI 1039 CTCGTG CACGAG(-5/-1)
3827 CTCGTG
MroI 4038 TCCGGA T^CCGGA
4521 TCCGGA
PmaCI 120 CACGTG CAC^GTG
2331 CACGTG
PmlI 120 CACGTG CAC^GTG
2331 CACGTG
SexAI 3431 ACCTGGT A^CC[AT]GGT
4394 ACCTGGT
TaqI 522 TCGA T^CGA
1313 TCGA
TthHB8I 522 TCGA T^CGA
1313 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TfiI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI