##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
CelII ClaI CpoI DraII DraIII
EagI EclXI Eco47III Eco52I EcoO109I
FbaI FseI FspI HaeII KasI
KpnI KspI MluI NaeI NarI
NgoMIV NotI NruI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SnaBI SpeI SspBI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 4756 CGCG CG^CG
AccIII 1221 TCCGGA T^CCGGA
AcyI 100 GACGCC G[GA]^CG[CT]C
AflII 4080 CTTAAG C^TTAAG
AhdI 2230 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2230 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4786 CCCGGG C^[CT]CG[GA]G
BanII 2449 GAGCTC G[GA]GC[CT]^C
BfrI 4080 CTTAAG C^TTAAG
BglII 462 AGATCT A^GATCT
BsaBI 4909 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 100 GACGCC G[GA]^CG[CT]C
BseAI 1221 TCCGGA T^CCGGA
BsmBI 4715 GAGACG CGTCTC(1/5)
BsoBI 4786 CCCGGG C^[CT]CG[GA]G
BspEI 1221 TCCGGA T^CCGGA
BsrFI 2059 ACCGGC [GA]^CCGG[CT]
BstAPI 711 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1349 TTCGAA TT^CGAA
BstUI 4756 CGCG CG^CG
BstXI 4369 CCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4755 GCGC GCG^C
Cfr10I 2059 ACCGGC [GA]^CCGG[CT]
DrdI 916 GACAGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2230 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3555 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4277 GATATC GAT^ATC
HhaI 4755 GCGC GCG^C
Hin1I 100 GACGCC G[GA]^CG[CT]C
HinP1I 4755 GCGC G^CGC
HpaI 1059 GTTAAC GTT^AAC
MaeII 3201 ACGT A^CGT
MamI 4909 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2287 CAATTG C^AATTG
MroI 1221 TCCGGA T^CCGGA
MslI 942 CATAACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2287 CAATTG C^AATTG
MvnI 4756 CGCG CG^CG
NheI 5079 GCTAGC G^CTAGC
NspV 1349 TTCGAA TT^CGAA
SacI 2449 GAGCTC GAGCT^C
SbfI 2364 CCTGCAGG CCTGCA^GG
ScaI 4095 AGTACT AGT^ACT
SfuI 1349 TTCGAA TT^CGAA
SmaI 4786 CCCGGG CCC^GGG
SmlI 4080 CTTAAG C^T[CT][GA]AG
SphI 4839 GCATGC GCATG^C
Sse8387I 2364 CCTGCAGG CCTGCA^GG
StuI 2417 AGGCCT AGG^CCT
XmaCI 4786 CCCGGG C^CCGGG
XmaI 4786 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 1122 CCTCAGG CC^T[ACGT]AGG
1447 CCTCAGG
AspI 2337 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4717 GACGGAGTC
BalI 3420 TGGCCA TGG^CCA
4900 TGGCCA
BanI 190 GGCACC G^G[CT][GA]CC
3645 GGCACC
BsaI 3223 GAGACC GGTCTC(1/5)
86 GGTCTC
BsgI 577 GTGCAG GTGCAG(16/14)
4673 GTGCAG
BsmI 516 GAATGC GAATGC(1/-1)
3497 GCATTC
BspLU11I 1214 ACATGT A^CATGT
2127 ACATGT
BssSI 2428 CTCGTG CACGAG(-5/-1)
4926 CTCGTG
Bst1107I 3083 GTATAC GTA^TAC
5266 GTATAC
BstEII 538 GGTGACC G^GT[ACGT]ACC
1899 GGTTACC
BstPI 538 GGTGACC G^GT[ACGT]ACC
1899 GGTTACC
BstYI 462 AGATCT [GA]^GATC[CT]
622 GGATCT
BstZ17I 3083 GTATAC GTA^TAC
5266 GTATAC
Bsu36I 1122 CCTCAGG CC^T[ACGT]AGG
1447 CCTCAGG
EaeI 3420 TGGCCA [CT]^GGCC[GA]
4900 TGGCCA
Eco81I 1122 CCTCAGG CC^T[ACGT]AGG
1447 CCTCAGG
EcoO65I 538 GGTGACC G^GT[ACGT]ACC
1899 GGTTACC
FauI 55 CCCGC CCCGC(4/6)
4937 CCCGC
MflI 462 AGATCT [GA]^GATC[CT]
622 GGATCT
MluNI 3420 TGGCCA TGG^CCA
4900 TGGCCA
MscI 3420 TGGCCA TGG^CCA
4900 TGGCCA
NdeI 3605 CATATG CA^TATG
3678 CATATG
PacI 3969 TTAATTAA TTAAT^TAA
4217 TTAATTAA
PflFI 2337 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4717 GACGGAGTC
PvuII 1535 CAGCTG CAG^CTG
5053 CAGCTG
SexAI 3518 ACCAGGT A^CC[AT]GGT
5118 ACCTGGT
SwaI 3902 ATTTAAAT ATTT^AAAT
4490 ATTTAAAT
Tth111I 2337 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4717 GACGGAGTC
XhoII 462 AGATCT [GA]^GATC[CT]
622 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmnI