##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI Asp718I AspEI AviII
BamHI BbeI BbrPI BcgI BfrI
BglI BglII BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
BstBI CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FseI FspI HaeII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NotI NruI
NspV PflMI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI Sse8387I Van91I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2878 GACGTC GACGT^C
AccII 38 CGCG CG^CG
AclI 2167 AACGTT AA^CGTT
ApaI 1222 GGGCCC GGGCC^C
AseI 4765 ATTAAT AT^TAAT
AsnI 4765 ATTAAT AT^TAAT
Asp700I 13 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2471 CCTAGG C^CTAGG
BlnI 2471 CCTAGG C^CTAGG
BsaBI 1800 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1604 GGTCTC GGTCTC(1/5)
BspHI 4833 TCATGA T^CATGA
BsrFI 371 GCCGGC [GA]^CCGG[CT]
Bst1107I 2683 GTATAC GTA^TAC
BstEII 1903 GGTTACC G^GT[ACGT]ACC
BstPI 1903 GGTTACC G^GT[ACGT]ACC
BstUI 38 CGCG CG^CG
BstXI 785 CCATGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2683 GTATAC GTA^TAC
Cfr10I 371 GCCGGC [GA]^CCGG[CT]
EcoO65I 1903 GGTTACC G^GT[ACGT]ACC
EcoRV 2179 GATATC GAT^ATC
EcoT22I 3053 ATGCAT ATGCA^T
MamI 1800 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 38 CGCG CG^CG
NaeI 371 GCCGGC GCC^GGC
NgoMIV 371 GCCGGC G^CCGGC
NheI 1274 GCTAGC G^CTAGC
NsiI 3053 ATGCAT ATGCA^T
PacI 5235 TTAATTAA TTAAT^TAA
PaeR7I 1001 CTCGAG C^TCGAG
PshAI 1071 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4765 ATTAAT AT^TAAT
Psp1406I 2167 AACGTT AA^CGTT
RcaI 4833 TCATGA T^CATGA
SacI 321 GAGCTC GAGCT^C
SmaI 429 CCCGGG CCC^GGG
SnaBI 1755 TACGTA TAC^GTA
SwaI 4063 ATTTAAAT ATTT^AAAT
XbaI 4702 TCTAGA T^CTAGA
XhoI 1001 CTCGAG C^TCGAG
XmaCI 429 CCCGGG C^CCGGG
XmaI 429 CCCGGG C^CCGGG
XmnI 13 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 2878 GACGTC G[GA]^CG[CT]C
4912 GACGCC
Alw44I 2815 GTGCAC G^TGCAC
4876 GTGCAC
AlwI 2268 GATCC GGATC(4/5)
2461 GATCC
AocI 1908 CCTGAGG CC^T[ACGT]AGG
3621 CCTCAGG
ApaLI 2815 GTGCAC G^TGCAC
4876 GTGCAC
AspI 2093 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACATTGTC
BbsI 4909 GAAGAC GAAGAC(2/6)
3382 GTCTTC
BbvCI 1826 CCTCAGC CCTCAGC(-5/-2)
3487 CCTCAGC
BclI 2291 TGATCA T^GATCA
4044 TGATCA
BpuAI 4909 GAAGAC GAAGAC(2/6)
3382 GTCTTC
BsaHI 2878 GACGTC G[GA]^CG[CT]C
4912 GACGCC
BspLU11I 5185 ACATGT A^CATGT
5252 ACATGT
BsrDI 2036 CATTGC GCAATG(2/0)
5096 CATTGC
BstYI 2267 AGATCC [GA]^GATC[CT]
2460 AGATCC
Bsu36I 1908 CCTGAGG CC^T[ACGT]AGG
3621 CCTCAGG
CfoI 39 GCGC GCG^C
3921 GCGC
DrdI 897 GACTGCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2878 GACGTCAAAGTC
Eco81I 1908 CCTGAGG CC^T[ACGT]AGG
3621 CCTCAGG
EcoNI 531 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3163 CCTTTACAAGG
FbaI 2291 TGATCA T^GATCA
4044 TGATCA
HhaI 39 GCGC GCG^C
3921 GCGC
Hin1I 2878 GACGTC G[GA]^CG[CT]C
4912 GACGCC
HinP1I 39 GCGC G^CGC
3921 GCGC
HindIII 2865 AAGCTT A^AGCTT
5242 AAGCTT
MflI 2267 AGATCC [GA]^GATC[CT]
2460 AGATCC
NdeI 1439 CATATG CA^TATG
3050 CATATG
PflFI 2093 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACATTGTC
SpeI 149 ACTAGT A^CTAGT
3898 ACTAGT
SphI 3055 GCATGC GCATG^C
3540 GCATGC
SspI 4057 AATATT AAT^ATT
4068 AATATT
StuI 15 AGGCCT AGG^CCT
2372 AGGCCT
Tth111I 2093 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3245 GACATTGTC
XhoII 2267 AGATCC [GA]^GATC[CT]
2460 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BciVI BcnI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI